Restriction map for
pFN21AE4374
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 852 GACGCC G[GA]^CG[CT]C
Alw44I 1039 GTGCAC G^TGCAC
AlwNI 964 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1039 GTGCAC G^TGCAC
Asp700I 315 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1011 CTCGGG C^[CT]CG[GA]G
BbsI 174 GAAGAC GAAGAC(2/6)
BglII 23 AGATCT A^GATCT
BlpI 386 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 386 GCTCAGC GC^T[ACGT]AGC
BpuAI 174 GAAGAC GAAGAC(2/6)
BsaHI 852 GACGCC G[GA]^CG[CT]C
BsaI 624 GGTCTC GGTCTC(1/5)
BsaWI 374 ACCGGA [AT]^CCGG[AT]
BsgI 262 CTGCAC GTGCAG(16/14)
BsmAI 625 GTCTC GTCTC(1/5)
BsoBI 1011 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1052 TGTACA T^GTACA
BsrBI 11 CCGCTC CCGCTC(-3/-3)
BsrGI 1052 TGTACA T^GTACA
BssSI 1156 CACGAG CACGAG(-5/-1)
BstXI 825 CCACGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 386 GCTCAGC GC^T[ACGT]AGC
DsaI 1082 CCATGG C^C[GA][CT]GG
EarI 349 CTCTTC CTCTTC(1/4)
EcoNI 978 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 852 GACGC GACGC(5/10)
Hin1I 852 GACGCC G[GA]^CG[CT]C
HindIII 37 AAGCTT A^AGCTT
Ksp632I 349 CTCTTC CTCTTC(1/4)
NcoI 1082 CCATGG C^CATGG
NspI 191 ACATGC [GA]CATG^[CT]
PleI 897 GAGTC GAGTC(4/5)
PshAI 618 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI 733 CTCAAG C^T[CT][GA]AG
SspBI 1052 TGTACA T^GTACA
Tsp509I 697 AATT ^AATT
XmnI 315 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 814 GTCTAC GT^[AC][GT]AC
899 GTCTAC
AfaI 1053 GTAC GT^AC
1140 GTAC
AlwI 1104 GATCC GGATC(4/5)
1134 GATCC
AspHI 514 GTGCTC G[AT]GC[AT]^C
1039 GTGCAC
AspI 469 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
893 GACAGAGTC
BsiHKAI 514 GTGCTC G[AT]GC[AT]^C
1039 GTGCAC
BspMI 107 ACCTGC ACCTGC(4/8)
339 GCAGGT
BsrFI 446 GCCGGC [GA]^CCGG[CT]
487 GCCGGC
BssHII 558 GCGCGC G^CGCGC
560 GCGCGC
BstAPI 963 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1016 GCACCGCTTGC
BstYI 23 AGATCT [GA]^GATC[CT]
1133 AGATCC
Cfr10I 446 GCCGGC [GA]^CCGG[CT]
487 GCCGGC
EaeI 2 TGGCCG [CT]^GGCC[GA]
635 TGGCCG
EcoT14I 52 CCTTGG C^C[AT][AT]GG
1082 CCATGG
MaeIII 616 GTGAC ^GT[ACGT]AC
850 GTGAC
MflI 23 AGATCT [GA]^GATC[CT]
1133 AGATCC
NaeI 446 GCCGGC GCC^GGC
487 GCCGGC
NgoMIV 446 GCCGGC G^CCGGC
487 GCCGGC
PflFI 469 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
893 GACAGAGTC
PpuMI 473 GGGTCCC [GA]G^G[AT]CC[CT]
752 AGGTCCT
PvuII 112 CAGCTG CAG^CTG
298 CAGCTG
RsaI 1053 GTAC GT^AC
1140 GTAC
StyI 52 CCTTGG C^C[AT][AT]GG
1082 CCATGG
TaqI 416 TCGA T^CGA
455 TCGA
Tsp45I 616 GTGAC ^GT[GC]AC
850 GTGAC
TspRI 587 CAGTG CA[GC]TG[ACGT][ACGT]^
1037 CAGTG
Tth111I 469 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
893 GACAGAGTC
TthHB8I 416 TCGA T^CGA
455 TCGA
XhoII 23 AGATCT [GA]^GATC[CT]
1133 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AluI ApaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI TseI