Restriction map for
pFN21AE4346
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# NO CUT ENZYMES ( 127 enzymes) #
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AccII AccIII AcsI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BamHI BbrPI BbsI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KspI MfeI MflI MluI MroI
MseI MunI MvnI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfuI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 625 GACGTC GACGT^C
Acc65I 905 GGTACC G^GTACC
AccI 706 GTCTAC GT^[AC][GT]AC
AclI 789 AACGTT AA^CGTT
AflIII 8 ACGTGT A^C[GA][CT]GT
AlwI 965 GGATC GGATC(4/5)
AocI 194 CCTCAGG CC^T[ACGT]AGG
ApaI 504 GGGCCC GGGCC^C
Asp718I 905 GGTACC G^GTACC
BalI 527 TGGCCA TGG^CCA
BbeI 133 GGCGCC GGCGC^C
BbvCI 360 CCTCAGC CCTCAGC(-5/-2)
BcgI 959 GCAACTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 748 CTAG C^TAG
BsaBI 525 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 511 GGTCTC GGTCTC(1/5)
BsaWI 260 ACCGGA [AT]^CCGG[AT]
BsmBI 894 CGTCTC CGTCTC(1/5)
BstXI 284 CCATTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 194 CCTCAGG CC^T[ACGT]AGG
DpnII 966 GATC ^GATC
EaeI 527 TGGCCA [CT]^GGCC[GA]
EarI 937 CTCTTC CTCTTC(1/4)
Eco81I 194 CCTCAGG CC^T[ACGT]AGG
EcoNI 918 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 893 GCGTC GACGC(5/10)
KasI 133 GGCGCC G^GCGCC
KpnI 905 GGTACC GGTAC^C
Ksp632I 937 CTCTTC CTCTTC(1/4)
MaeI 748 CTAG C^TAG
MamI 525 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 966 GATC ^GATC
MluNI 527 TGGCCA TGG^CCA
MscI 527 TGGCCA TGG^CCA
NarI 133 GGCGCC GG^CGCC
NdeI 401 CATATG CA^TATG
NdeII 966 GATC ^GATC
NspI 806 GCATGC [GA]CATG^[CT]
Psp1406I 789 AACGTT AA^CGTT
PstI 844 CTGCAG CTGCA^G
Sau3AI 966 GATC ^GATC
SfcI 844 CTGCAG C^T[GA][CT]AG
SfiI 1017 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 133 GGCGCC GGC^GCC
SgrAI 712 CACCGGCG C[GA]^CCGG[CT]G
SphI 806 GCATGC GCATG^C
XcmI 711 CCACCGGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 133 GGCGCC G[GA]^CG[CT]C
625 GACGTC
AvaI 172 CCCGGG C^[CT]CG[GA]G
237 CCCGGG
BanII 504 GGGCCC G[GA]GC[CT]^C
585 GGGCTC
BsaHI 133 GGCGCC G[GA]^CG[CT]C
625 GACGTC
BsmFI 409 GGGAC GGGAC(10/14)
425 GTCCC
BsoBI 172 CCCGGG C^[CT]CG[GA]G
237 CCCGGG
BssSI 64 CTCGTG CACGAG(-5/-1)
631 CTCGTG
DrdI 537 GACCCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
886 GACACCAGCGTC
EcoT14I 923 CCAAGG C^C[AT][AT]GG
1020 CCTTGG
FauI 31 CCCGC CCCGC(4/6)
802 GCGGG
HaeII 133 GGCGCC [GA]GCGC^[CT]
190 AGCGCC
Hin1I 133 GGCGCC G[GA]^CG[CT]C
625 GACGTC
HincII 313 GTCAAC GT[CT]^[GA]AC
343 GTCAAC
HindII 313 GTCAAC GT[CT]^[GA]AC
343 GTCAAC
MboII 987 GAAGA GAAGA(8/7)
938 TCTTC
NaeI 57 GCCGGC GCC^GGC
136 GCCGGC
NgoMIV 57 GCCGGC G^CCGGC
136 GCCGGC
SfaNI 879 GATGC GCATC(5/9)
671 GCATC
SmaI 172 CCCGGG CCC^GGG
237 CCCGGG
StyI 923 CCAAGG C^C[AT][AT]GG
1020 CCTTGG
TfiI 644 GAATC G^A[AT]TC
1035 GAATC
XmaCI 172 CCCGGG C^CCGGG
237 CCCGGG
XmaI 172 CCCGGG C^CCGGG
237 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MnlI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PleI PvuII RsaI Sau96I
ScrFI TaqI TseI Tsp45I TspRI
TthHB8I