##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
ItaI        KasI        KpnI        Ksp632I     KspI        
MamI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
TseI        TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AflIII        216  ACATGT               A^C[GA][CT]GT
AluI          345  AGCT                 AG^CT
AlwI          269  GGATC                GGATC(4/5)
AvaII           3  GGTCC                G^G[AT]CC
BfaI           59  CTAG                 C^TAG
BmyI          144  GTGCCC               G[AGT]GC[ACT]^C
BseRI         292  GAGGAG               GAGGAG(10/8)
BsiYI         285  CCTTATCGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          285  CCTTATCGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          66  GTCCC                GGGAC(10/14)
Bsp1286I      144  GTGCCC               G[AGT]GC[ACT]^C
BspLU11I      216  ACATGT               A^CATGT
Cac8I         276  GCGTGC               GC[ACGT]^[ACGT]GC
DpnII         270  GATC                 ^GATC
DraII         226  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoNI         285  CCTTATCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      226  AGGGCCT              [GA]G^G[ACGT]CC[CT]
HaeIII        228  GGCC                 GG^CC
HinfI         336  GAGTC                G^A[ACGT]TC
MaeI           59  CTAG                 C^TAG
MaeII          24  ACGT                 A^CGT
MaeIII        263  GTGAC                ^GT[ACGT]AC
MboI          270  GATC                 ^GATC
NdeII         270  GATC                 ^GATC
NlaIV         238  GGTTCC               GG[ACGT]^[ACGT]CC
PleI          336  GAGTC                GAGTC(4/5)
Sau3AI        270  GATC                 ^GATC
Tsp45I        263  GTGAC                ^GT[GC]AC
Tsp509I        87  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BsaJI          75  CCCTGG               C^C[ACGT][ACGT]GG
              147  CCCAGG              
BssKI          76  CCTGG                ^CC[ACGT]GG
              148  CCAGG               
BstNI          76  CCTGG                CC^[AT]GG
              148  CCAGG               
Cfr13I          3  GGTCC                G^G[ACGT]CC
              227  GGGCC               
EcoRII         76  CCTGG                ^CC[AT]GG
              148  CCAGG               
MvaI           76  CCTGG                CC^[AT]GG
              148  CCAGG               
NspI          133  GCATGT               [GA]CATG^[CT]
              216  ACATGT              
Sau96I          3  GGTCC                G^G[ACGT]CC
              227  GGGCC               
ScrFI          76  CCTGG                CC^[ACGT]GG
              148  CCAGG               
TaqI          159  TCGA                 T^CGA
              290  TCGA                
TthHB8I       159  TCGA                 T^CGA
              290  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MnlI        NlaIII