Restriction map for
pFN21AE1451
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I EarI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI Ksp632I MaeI MflI
MluI MroI MseI MslI NarI
NcoI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I TthHB8I XbaI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Alw44I 1044 GTGCAC G^TGCAC
AlwI 562 GGATC GGATC(4/5)
AlwNI 52 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 995 AGCGCT AGC^GCT
ApaLI 1044 GTGCAC G^TGCAC
AspI 829 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 866 TGCGCA TGC^GCA
BalI 168 TGGCCA TGG^CCA
BanI 127 GGTGCC G^G[CT][GA]CC
BcgI 1046 GCACGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 398 GCCGCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 49 GCTCAGC GC^T[ACGT]AGC
BpmI 787 CTGGAG CTGGAG(16/14)
Bpu1102I 49 GCTCAGC GC^T[ACGT]AGC
BsaBI 114 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 568 GAGGAG GAGGAG(10/8)
BsmBI 663 CGTCTC CGTCTC(1/5)
BsmI 244 GAATGC GAATGC(1/-1)
BspHI 587 TCATGA T^CATGA
CelII 49 GCTCAGC GC^T[ACGT]AGC
DdeI 50 CTCAG C^T[ACGT]AG
DraIII 352 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 755 CGGCCG C^GGCCG
EclXI 755 CGGCCG C^GGCCG
Eco47III 995 AGCGCT AGC^GCT
Eco52I 755 CGGCCG C^GGCCG
FspI 866 TGCGCA TGC^GCA
HinfI 314 GAGTC G^A[ACGT]TC
KspI 365 CCGCGG CCGC^GG
MaeIII 769 GTCAC ^GT[ACGT]AC
MamI 114 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 546 GAAGA GAAGA(8/7)
MfeI 187 CAATTG C^AATTG
MluNI 168 TGGCCA TGG^CCA
MscI 168 TGGCCA TGG^CCA
MunI 187 CAATTG C^AATTG
NaeI 782 GCCGGC GCC^GGC
NgoMIV 782 GCCGGC G^CCGGC
NotI 754 GCGGCCGC GC^GGCCGC
PflFI 829 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 68 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 314 GAGTC GAGTC(4/5)
RcaI 587 TCATGA T^CATGA
SacII 365 CCGCGG CCGC^GG
SexAI 830 ACCTGGT A^CC[AT]GGT
SmlI 682 CTCAAG C^T[CT][GA]AG
Tsp45I 769 GTCAC ^GT[GC]AC
Tth111I 829 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 68 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 515 CCAAGGAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 64 GACGCC G[GA]^CG[CT]C
661 GGCGTC
BsaHI 64 GACGCC G[GA]^CG[CT]C
661 GGCGTC
BsaI 105 GAGACC GGTCTC(1/5)
337 GAGACC
BsiEI 612 CGGTCG CG[GA][CT]^CG
755 CGGCCG
BstF5I 528 GGATG GGATG(2/0)
933 GGATG
DpnII 563 GATC ^GATC
1053 GATC
DsaI 365 CCGCGG C^C[GA][CT]GG
657 CCACGG
EcoT14I 446 CCAAGG C^C[AT][AT]GG
515 CCAAGG
FokI 528 GGATG GGATG(9/13)
933 GGATG
HaeII 361 AGCGCC [GA]GCGC^[CT]
995 AGCGCT
Hin1I 64 GACGCC G[GA]^CG[CT]C
661 GGCGTC
HincII 376 GTCAAC GT[CT]^[GA]AC
796 GTCAAC
HindII 376 GTCAAC GT[CT]^[GA]AC
796 GTCAAC
MaeII 491 ACGT A^CGT
641 ACGT
MboI 563 GATC ^GATC
1053 GATC
NdeII 563 GATC ^GATC
1053 GATC
PvuII 42 CAGCTG CAG^CTG
700 CAGCTG
SacI 409 GAGCTC GAGCT^C
943 GAGCTC
Sau3AI 563 GATC ^GATC
1053 GATC
StyI 446 CCAAGG C^C[AT][AT]GG
515 CCAAGG
Tsp509I 188 AATT ^AATT
277 AATT
TspRI 1003 CACTG CA[GC]TG[ACGT][ACGT]^
1015 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PpuMI PstI
RsaI Sau96I ScrFI SfaNI SfcI
SmaI TseI XmaCI XmaI