Restriction map for
pFN21AE6243
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AvaI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MscI MunI MvnI
NarI NcoI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 451 GGTACC G^GTACC
Asp718I 451 GGTACC G^GTACC
AspI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 491 GGATCC G^GATCC
BanI 451 GGTACC G^G[CT][GA]CC
BanII 616 GAGCTC G[GA]GC[CT]^C
BbsI 180 GAAGAC GAAGAC(2/6)
BbvCI 756 GCTGAGG CCTCAGC(-5/-2)
BcnI 771 CCGGG CC^[GC]GG
BfaI 545 CTAG C^TAG
BglI 835 GCCAATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 321 GCTCAGC GC^T[ACGT]AGC
BpmI 389 CTGGAG CTGGAG(16/14)
Bpu1102I 321 GCTCAGC GC^T[ACGT]AGC
BpuAI 180 GAAGAC GAAGAC(2/6)
BsgI 716 CTGCAC GTGCAG(16/14)
BsmAI 702 GTCTC GTCTC(1/5)
Bsp1407I 794 TGTACA T^GTACA
BspMI 38 ACCTGC ACCTGC(4/8)
BsrBI 28 CCGCTC CCGCTC(-3/-3)
BsrDI 651 CATTGC GCAATG(2/0)
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BsrGI 794 TGTACA T^GTACA
BstEII 82 GGTCACC G^GT[ACGT]ACC
BstPI 82 GGTCACC G^GT[ACGT]ACC
BstXI 480 CCAGGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 321 GCTCAGC GC^T[ACGT]AGC
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
DraII 160 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 160 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 82 GGTCACC G^GT[ACGT]ACC
FauI 27 CCCGC CCCGC(4/6)
KpnI 451 GGTACC GGTAC^C
MaeI 545 CTAG C^TAG
MseI 881 TTAA T^TAA
MspA1I 344 CAGCTG C[AC]G^C[GT]G
NaeI 46 GCCGGC GCC^GGC
NciI 771 CCGGG CC^[GC]GG
NdeI 566 CATATG CA^TATG
NgoMIV 46 GCCGGC G^CCGGC
PflFI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 541 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 160 GGGACCT [GA]G^G[AT]CC[CT]
PstI 112 CTGCAG CTGCA^G
PvuII 344 CAGCTG CAG^CTG
SacI 616 GAGCTC GAGCT^C
SfcI 112 CTGCAG C^T[GA][CT]AG
SspBI 794 TGTACA T^GTACA
TaqI 705 TCGA T^CGA
Tru9I 881 TTAA T^TAA
Tsp509I 883 AATT ^AATT
Tth111I 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 705 TCGA T^CGA
Van91I 541 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 176 TCCGGA T^CCGGA
594 TCCGGA
AvaII 105 GGTCC G^G[AT]CC
161 GGACC
BbvI 343 GCAGC GCAGC(8/12)
226 GCTGC
BsaWI 176 TCCGGA [AT]^CCGG[AT]
594 TCCGGA
BseAI 176 TCCGGA T^CCGGA
594 TCCGGA
BspEI 176 TCCGGA T^CCGGA
594 TCCGGA
BspHI 380 TCATGA T^CATGA
560 TCATGA
BsrI 448 ACTGG ACTGG(1/-1)
668 ACTGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
44 TGGCCG
EcoT14I 455 CCTTGG C^C[AT][AT]GG
852 CCTTGG
HphI 84 TCACC GGTGA(8/7)
368 TCACC
MroI 176 TCCGGA T^CCGGA
594 TCCGGA
MslI 246 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
566 CATATGAATG
RcaI 380 TCATGA T^CATGA
560 TCATGA
SfaNI 200 GCATC GCATC(5/9)
500 GCATC
SmlI 31 CTCAAG C^T[CT][GA]AG
697 CTCAAG
StyI 455 CCTTGG C^C[AT][AT]GG
852 CCTTGG
TseI 226 GCTGC G^C[AT]GC
343 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI TfiI
Tsp45I TspRI XhoII