Restriction map for
pFN21AE1377
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspI BbeI
BbrPI BcgI BciVI BclI BglI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FauI FbaI FseI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 1366 CTTAAG C^TTAAG
AhdI 1129 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1066 GTGCAC G^TGCAC
AlwNI 21 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1021 CCTAAGG CC^T[ACGT]AGG
ApaLI 1066 GTGCAC G^TGCAC
AspEI 1129 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1540 TGCGCA TGC^GCA
AvrII 594 CCTAGG C^CTAGG
BamHI 899 GGATCC G^GATCC
BanI 1206 GGTGCC G^G[CT][GA]CC
BfrI 1366 CTTAAG C^TTAAG
BglII 704 AGATCT A^GATCT
BlnI 594 CCTAGG C^CTAGG
BseRI 956 CTCCTC GAGGAG(10/8)
BsiEI 608 CGGTCG CG[GA][CT]^CG
Bsp1407I 137 TGTACA T^GTACA
BspHI 1088 TCATGA T^CATGA
BsrGI 137 TGTACA T^GTACA
BssSI 441 CACGAG CACGAG(-5/-1)
BstEII 1291 GGTGACC G^GT[ACGT]ACC
BstPI 1291 GGTGACC G^GT[ACGT]ACC
Bsu36I 1021 CCTAAGG CC^T[ACGT]AGG
CfoI 1541 GCGC GCG^C
DraIII 733 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 833 CCACGG C^C[GA][CT]GG
Eam1105I 1129 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1021 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1291 GGTGACC G^GT[ACGT]ACC
FspI 1540 TGCGCA TGC^GCA
HhaI 1541 GCGC GCG^C
HinP1I 1541 GCGC G^CGC
NspI 134 GCATGT [GA]CATG^[CT]
RcaI 1088 TCATGA T^CATGA
SmaI 1102 CCCGGG CCC^GGG
SspBI 137 TGTACA T^GTACA
StuI 173 AGGCCT AGG^CCT
TfiI 294 GATTC G^A[AT]TC
XmaCI 1102 CCCGGG C^CCGGG
XmaI 1102 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 138 GTAC GT^AC
1077 GTAC
AvaI 1102 CCCGGG C^[CT]CG[GA]G
1187 CTCGGG
BalI 437 TGGCCA TGG^CCA
578 TGGCCA
BbvCI 907 CCTCAGC CCTCAGC(-5/-2)
1360 CCTCAGC
BfaI 595 CTAG C^TAG
962 CTAG
BsoBI 1102 CCCGGG C^[CT]CG[GA]G
1187 CTCGGG
DraII 98 AGGACCC [GA]G^G[ACGT]CC[CT]
1421 AGGACCT
EaeI 437 TGGCCA [CT]^GGCC[GA]
578 TGGCCA
EcoNI 6 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
594 CCTAGGCAAGG
EcoO109I 98 AGGACCC [GA]G^G[ACGT]CC[CT]
1421 AGGACCT
MaeI 595 CTAG C^TAG
962 CTAG
MaeIII 993 GTCAC ^GT[ACGT]AC
1292 GTGAC
MluNI 437 TGGCCA TGG^CCA
578 TGGCCA
MscI 437 TGGCCA TGG^CCA
578 TGGCCA
MslI 441 CACGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CACGGTCATG
PleI 1095 GACTC GAGTC(4/5)
1266 GACTC
PpuMI 98 AGGACCC [GA]G^G[AT]CC[CT]
1421 AGGACCT
RsaI 138 GTAC GT^AC
1077 GTAC
SacI 772 GAGCTC GAGCT^C
1150 GAGCTC
SapI 769 GAAGAGC GCTCTTC(1/4)
1015 GAAGAGC
SfcI 1113 CTACAG C^T[GA][CT]AG
1547 CTACAG
SmlI 1060 CTCAAG C^T[CT][GA]AG
1366 CTTAAG
Tsp45I 993 GTCAC ^GT[GC]AC
1292 GTGAC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbsI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI TspRI TthHB8I XhoII