Restriction map for
pFN21AE5610
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbvCI BfrI BlnI BsaBI
BsaHI BsaI BsiWI BsmBI BsmI
BspDI BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MluI
MslI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmeI
PpuMI PshBI Psp1406I RsrII SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tth111I Van91I XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 1520 TCCGGA T^CCGGA
AciI 531 GCGG CCGC(-3/-1)
AflIII 887 ACATGT A^C[GA][CT]GT
AocI 373 CCTCAGG CC^T[ACGT]AGG
BalI 1194 TGGCCA TGG^CCA
BamHI 1305 GGATCC G^GATCC
BbrPI 695 CACGTG CAC^GTG
BcgI 390 CGACCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 237 GTATCC GTATCC(6/5)
BclI 105 TGATCA T^GATCA
BcnI 1429 CCCGG CC^[GC]GG
BglI 1609 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 588 AGATCT A^GATCT
BlpI 1002 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1002 GCTGAGC GC^T[ACGT]AGC
BsaWI 1520 TCCGGA [AT]^CCGG[AT]
BseAI 1520 TCCGGA T^CCGGA
BsgI 1636 GTGCAG GTGCAG(16/14)
BsiEI 1238 CGATCG CG[GA][CT]^CG
Bsp1407I 248 TGTACA T^GTACA
BspEI 1520 TCCGGA T^CCGGA
BspHI 1355 TCATGA T^CATGA
BspLU11I 887 ACATGT A^CATGT
BsrGI 248 TGTACA T^GTACA
BstAPI 963 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 373 CCTCAGG CC^T[ACGT]AGG
CelII 1002 GCTGAGC GC^T[ACGT]AGC
DraIII 252 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 373 CCTCAGG CC^T[ACGT]AGG
FbaI 105 TGATCA T^GATCA
HgaI 1495 GCGTC GACGC(5/10)
MfeI 1469 CAATTG C^AATTG
MluNI 1194 TGGCCA TGG^CCA
MroI 1520 TCCGGA T^CCGGA
MscI 1194 TGGCCA TGG^CCA
MseI 1247 TTAA T^TAA
MspA1I 458 CAGCTG C[AC]G^C[GT]G
MunI 1469 CAATTG C^AATTG
NciI 1429 CCCGG CC^[GC]GG
NcoI 1366 CCATGG C^CATGG
NheI 1588 GCTAGC G^CTAGC
PaeR7I 307 CTCGAG C^TCGAG
PmaCI 695 CACGTG CAC^GTG
PmlI 695 CACGTG CAC^GTG
PshAI 1512 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1238 CGATCG CGAT^CG
PvuII 458 CAGCTG CAG^CTG
RcaI 1355 TCATGA T^CATGA
SacI 139 GAGCTC GAGCT^C
SapI 1179 GCTCTTC GCTCTTC(1/4)
SbfI 630 CCTGCAGG CCTGCA^GG
SmlI 307 CTCGAG C^T[CT][GA]AG
Sse8387I 630 CCTGCAGG CCTGCA^GG
SspBI 248 TGTACA T^GTACA
Tru9I 1247 TTAA T^TAA
XbaI 1719 TCTAGA T^CTAGA
XhoI 307 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 179 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
1556 CAGTGGCTG
BsaAI 190 TACGTG [CT]AC^GT[GA]
695 CACGTG
BseRI 136 GAGGAG GAGGAG(10/8)
670 GAGGAG
BsmFI 1370 GGGAC GGGAC(10/14)
1159 GTCCC
BsrDI 561 CATTGC GCAATG(2/0)
807 GCAATG
BstEII 202 GGTAACC G^GT[ACGT]ACC
1509 GGTGACC
BstPI 202 GGTAACC G^GT[ACGT]ACC
1509 GGTGACC
DraII 743 GGGGCCT [GA]G^G[ACGT]CC[CT]
1727 AGGCCCT
DsaI 1366 CCATGG C^C[GA][CT]GG
1514 CCGTGG
EaeI 35 CGGCCA [CT]^GGCC[GA]
1194 TGGCCA
EcoO109I 743 GGGGCCT [GA]G^G[ACGT]CC[CT]
1727 AGGCCCT
EcoO65I 202 GGTAACC G^GT[ACGT]ACC
1509 GGTGACC
HapII 1430 CCGG C^CGG
1521 CCGG
HindIII 61 AAGCTT A^AGCTT
1360 AAGCTT
HpaII 1430 CCGG C^CGG
1521 CCGG
MspI 1430 CCGG C^CGG
1521 CCGG
NspI 819 ACATGC [GA]CATG^[CT]
887 ACATGT
PstI 631 CTGCAG CTGCA^G
961 CTGCAG
TfiI 580 GATTC G^A[AT]TC
1460 GAATC
Tsp509I 1400 AATT ^AATT
1470 AATT
XcmI 401 CCATTTCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
560 CCATTGCTCCACTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AfaI AluI AlwI AspHI AvaI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII