Restriction map for
pFN21AE6442
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BpuAI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI Eam1105I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HinfI HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 832 GTCTAC GT^[AC][GT]AC
Aor51HI 896 AGCGCT AGC^GCT
AspHI 1211 GTGCTC G[AT]GC[AT]^C
AviII 1199 TGCGCA TGC^GCA
AvrII 1206 CCTAGG C^CTAGG
BamHI 986 GGATCC G^GATCC
BbrPI 1287 CACGTG CAC^GTG
BlnI 1206 CCTAGG C^CTAGG
BsaAI 1287 CACGTG [CT]AC^GT[GA]
BsaI 268 GGTCTC GGTCTC(1/5)
BseRI 1051 CTCCTC GAGGAG(10/8)
BsgI 24 CTGCAC GTGCAG(16/14)
BsiEI 159 CGGCCG CG[GA][CT]^CG
BsiHKAI 1211 GTGCTC G[AT]GC[AT]^C
BsmBI 670 GAGACG CGTCTC(1/5)
BsmFI 1065 GGGAC GGGAC(10/14)
BsmI 283 GCATTC GAATGC(1/-1)
BspMI 641 ACCTGC ACCTGC(4/8)
BssSI 773 CTCGTG CACGAG(-5/-1)
BstEII 72 GGTCACC G^GT[ACGT]ACC
BstPI 72 GGTCACC G^GT[ACGT]ACC
BstYI 986 GGATCC [GA]^GATC[CT]
DraIII 1284 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 159 CGGCCG C^GGCCG
EclXI 159 CGGCCG C^GGCCG
Eco47III 896 AGCGCT AGC^GCT
Eco52I 159 CGGCCG C^GGCCG
EcoNI 915 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 72 GGTCACC G^GT[ACGT]ACC
FspI 1199 TGCGCA TGC^GCA
KspI 1226 CCGCGG CCGC^GG
MflI 986 GGATCC [GA]^GATC[CT]
MspA1I 1226 CCGCGG C[AC]G^C[GT]G
NcoI 953 CCATGG C^CATGG
NdeI 389 CATATG CA^TATG
PmaCI 1287 CACGTG CAC^GTG
PmlI 1287 CACGTG CAC^GTG
PpuMI 782 AGGACCC [GA]G^G[AT]CC[CT]
SacII 1226 CCGCGG CCGC^GG
SapI 624 GCTCTTC GCTCTTC(1/4)
SfiI 423 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 139 CCCGGG CCC^GGG
SmlI 1354 CTCAAG C^T[CT][GA]AG
StuI 1274 AGGCCT AGG^CCT
Tsp45I 73 GTCAC ^GT[GC]AC
XhoII 986 GGATCC [GA]^GATC[CT]
XmaCI 139 CCCGGG C^CCGGG
XmaI 139 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 347 GGCGCC G[GA]^CG[CT]C
693 GGCGCC
AlwI 987 GATCC GGATC(4/5)
986 GGATC
AlwNI 298 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1327 CAGAAGCTG
AvaI 139 CCCGGG C^[CT]CG[GA]G
858 CTCGGG
BbeI 347 GGCGCC GGCGC^C
693 GGCGCC
BfaI 829 CTAG C^TAG
1207 CTAG
BglI 424 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
818 GCCGCCTGGGC
BsaHI 347 GGCGCC G[GA]^CG[CT]C
693 GGCGCC
BsoBI 139 CCCGGG C^[CT]CG[GA]G
858 CTCGGG
DpnII 730 GATC ^GATC
987 GATC
EcoT14I 953 CCATGG C^C[AT][AT]GG
1206 CCTAGG
HgaI 672 GACGC GACGC(5/10)
1350 GACGC
Hin1I 347 GGCGCC G[GA]^CG[CT]C
693 GGCGCC
KasI 347 GGCGCC G^GCGCC
693 GGCGCC
MaeI 829 CTAG C^TAG
1207 CTAG
MaeIII 73 GTCAC ^GT[ACGT]AC
1337 GTTAC
MboI 730 GATC ^GATC
987 GATC
NarI 347 GGCGCC GG^CGCC
693 GGCGCC
NdeII 730 GATC ^GATC
987 GATC
PflMI 313 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
787 CCACCCTCTGG
PstI 295 CTGCAG CTGCA^G
1324 CTGCAG
Sau3AI 730 GATC ^GATC
987 GATC
SfoI 347 GGCGCC GGC^GCC
693 GGCGCC
StyI 953 CCATGG C^C[AT][AT]GG
1206 CCTAGG
Van91I 313 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
787 CCACCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaII BalI BanI BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeII MboII
MluNI MnlI MscI MslI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
SfcI TseI TspRI