Restriction map for
pFN21AE6419
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII AccI AccII AccIII AclI
AgeI Alw44I AocI Aor51HI ApaLI
AscI AspI AvaI AviII AvrII
BalI BbrPI BbsI BcgI BciVI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiWI
BsmBI BsoBI Bsp1407I BspEI BspHI
BspMI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CpoI DraI
DraIII DrdI DsaI EarI Eco47III
Eco81I EcoO65I EcoRI EcoT22I FspI
HgaI HincII HindII HindIII HpaI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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Acc65I 212 GGTACC G^GTACC
AcsI 426 GAATTT [GA]^AATT[CT]
AcyI 42 GGCGCC G[GA]^CG[CT]C
AflII 75 CTTAAG C^TTAAG
AflIII 1133 ACATGT A^C[GA][CT]GT
AhdI 1288 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 426 GAATTT [GA]^AATT[CT]
AseI 730 ATTAAT AT^TAAT
AsnI 730 ATTAAT AT^TAAT
Asp700I 975 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 212 GGTACC G^GTACC
AspEI 1288 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 593 GAGCTC G[AT]GC[AT]^C
BamHI 1395 GGATCC G^GATCC
BbeI 42 GGCGCC GGCGC^C
BbvCI 328 GCTGAGG CCTCAGC(-5/-2)
BclI 171 TGATCA T^GATCA
BcnI 123 CCCGG CC^[GC]GG
BfrI 75 CTTAAG C^TTAAG
BglII 1371 AGATCT A^GATCT
BpmI 773 CTGGAG CTGGAG(16/14)
BsaHI 42 GGCGCC G[GA]^CG[CT]C
BsaI 1200 GAGACC GGTCTC(1/5)
BsgI 1089 CTGCAC GTGCAG(16/14)
BsiEI 67 CGGCCG CG[GA][CT]^CG
BsiHKAI 593 GAGCTC G[AT]GC[AT]^C
BsmFI 281 GGGAC GGGAC(10/14)
BsmI 1386 GAATGC GAATGC(1/-1)
BspDI 365 ATCGAT AT^CGAT
BspLU11I 1133 ACATGT A^CATGT
BsrBI 1419 CCGCTC CCGCTC(-3/-3)
BsrFI 69 GCCGGC [GA]^CCGG[CT]
BstAPI 601 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 43 GCGC GCG^C
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
ClaI 365 ATCGAT AT^CGAT
EaeI 67 CGGCCG [CT]^GGCC[GA]
EagI 67 CGGCCG C^GGCCG
Eam1105I 1288 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 67 CGGCCG C^GGCCG
Eco52I 67 CGGCCG C^GGCCG
EcoNI 557 CCTGGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1318 GATATC GAT^ATC
FbaI 171 TGATCA T^GATCA
FseI 68 GGCCGGCC GGCCGG^CC
HaeII 42 GGCGCC [GA]GCGC^[CT]
HhaI 43 GCGC GCG^C
Hin1I 42 GGCGCC G[GA]^CG[CT]C
HinP1I 43 GCGC G^CGC
KasI 42 GGCGCC G^GCGCC
KpnI 212 GGTACC GGTAC^C
MslI 1221 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 208 CAGCGG C[AC]G^C[GT]G
NaeI 69 GCCGGC GCC^GGC
NarI 42 GGCGCC GG^CGCC
NciI 123 CCCGG CC^[GC]GG
NgoMIV 69 GCCGGC G^CCGGC
PpuMI 715 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 730 ATTAAT AT^TAAT
PstI 970 CTGCAG CTGCA^G
SacI 593 GAGCTC GAGCT^C
SfiI 72 GGCCTTAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 42 GGCGCC GGC^GCC
SmlI 75 CTTAAG C^T[CT][GA]AG
SphI 545 GCATGC GCATG^C
StuI 1085 AGGCCT AGG^CCT
XmnI 975 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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ApaI 80 GGGCCC GGGCC^C
1358 GGGCCC
AvaII 688 GGTCC G^G[AT]CC
716 GGTCC
BanI 42 GGCGCC G^G[CT][GA]CC
212 GGTACC
BglI 73 GCCTTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
903 GCCTGCTTGGC
FauI 1418 CCCGC CCCGC(4/6)
1474 GCGGG
NdeI 580 CATATG CA^TATG
1438 CATATG
NspI 545 GCATGC [GA]CATG^[CT]
1133 ACATGT
PleI 737 GACTC GAGTC(4/5)
379 GAGTC
SfaNI 724 GATGC GCATC(5/9)
707 GCATC
SspI 976 AATATT AAT^ATT
1033 AATATT
Tsp45I 395 GTGAC ^GT[GC]AC
1342 GTCAC
XcmI 1204 CCAAGGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1382 CCAAGAATGCCTTGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanII BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII