Restriction map for
pFN21AE4333
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BciVI BcnI BfrI BglII BlnI
BpuAI BsaAI BsaBI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CfoI ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MamI
MboII MfeI MluI MroI MseI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 749 GGTACC G^GTACC
AflIII 598 ACGTGT A^C[GA][CT]GT
AocI 631 CCTGAGG CC^T[ACGT]AGG
Asp718I 749 GGTACC G^GTACC
AvaI 571 CTCGAG C^[CT]CG[GA]G
AvaII 15 GGTCC G^G[AT]CC
BalI 44 TGGCCA TGG^CCA
BamHI 839 GGATCC G^GATCC
BbvCI 960 GCTGAGG CCTCAGC(-5/-2)
BcgI 146 GCAGTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 584 TGATCA T^GATCA
BfaI 313 CTAG C^TAG
BglI 781 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1017 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1017 GCTCAGC GC^T[ACGT]AGC
BsaI 742 GGTCTC GGTCTC(1/5)
BsaWI 71 TCCGGT [AT]^CCGG[AT]
BsiEI 73 CGGTCG CG[GA][CT]^CG
BsmBI 595 GAGACG CGTCTC(1/5)
BsoBI 571 CTCGAG C^[CT]CG[GA]G
BspMI 174 GCAGGT ACCTGC(4/8)
BsrBI 86 GAGCGG CCGCTC(-3/-3)
BsrDI 402 CATTGC GCAATG(2/0)
BsrFI 157 GCCGGT [GA]^CCGG[CT]
BsrI 511 ACTGG ACTGG(1/-1)
BstAPI 231 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 201 CCATGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 839 GGATCC [GA]^GATC[CT]
Bsu36I 631 CCTGAGG CC^T[ACGT]AGG
CelII 1017 GCTCAGC GC^T[ACGT]AGC
Cfr10I 157 GCCGGT [GA]^CCGG[CT]
DrdI 331 GACGCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1012 CCATGG C^C[GA][CT]GG
EaeI 44 TGGCCA [CT]^GGCC[GA]
Eco81I 631 CCTGAGG CC^T[ACGT]AGG
EcoNI 378 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 584 TGATCA T^GATCA
KpnI 749 GGTACC GGTAC^C
MaeI 313 CTAG C^TAG
MaeII 598 ACGT A^CGT
MflI 839 GGATCC [GA]^GATC[CT]
MluNI 44 TGGCCA TGG^CCA
MscI 44 TGGCCA TGG^CCA
MslI 202 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1012 CCATGG C^CATGG
PaeR7I 571 CTCGAG C^TCGAG
PpuMI 14 GGGTCCT [GA]G^G[AT]CC[CT]
SmlI 571 CTCGAG C^T[CT][GA]AG
StuI 799 AGGCCT AGG^CCT
XhoI 571 CTCGAG C^TCGAG
XhoII 839 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 840 GATCC GGATC(4/5)
839 GGATC
AspHI 268 GAGCAC G[AT]GC[AT]^C
1024 GAGCAC
BsgI 501 CTGCAC GTGCAG(16/14)
149 GTGCAG
BsiHKAI 268 GAGCAC G[AT]GC[AT]^C
1024 GAGCAC
BsmAI 595 GAGAC GTCTC(1/5)
743 GTCTC
BsmFI 79 GGGAC GGGAC(10/14)
894 GTCCC
Bsp1407I 611 TGTACA T^GTACA
809 TGTACA
BsrGI 611 TGTACA T^GTACA
809 TGTACA
BssSI 220 CTCGTG CACGAG(-5/-1)
869 CTCGTG
DpnII 585 GATC ^GATC
840 GATC
EcoT14I 335 CCAAGG C^C[AT][AT]GG
1012 CCATGG
FauI 440 CCCGC CCCGC(4/6)
791 CCCGC
MboI 585 GATC ^GATC
840 GATC
MspA1I 31 CAGCTG C[AC]G^C[GT]G
245 CAGCTG
NdeII 585 GATC ^GATC
840 GATC
PstI 467 CTGCAG CTGCA^G
562 CTGCAG
PvuII 31 CAGCTG CAG^CTG
245 CAGCTG
Sau3AI 585 GATC ^GATC
840 GATC
SfcI 467 CTGCAG C^T[GA][CT]AG
562 CTGCAG
SspBI 611 TGTACA T^GTACA
809 TGTACA
StyI 335 CCAAGG C^C[AT][AT]GG
1012 CCATGG
TaqI 572 TCGA T^CGA
620 TCGA
TthHB8I 572 TCGA T^CGA
620 TCGA
XcmI 201 CCATGTGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
702 CCAGGGTGACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcyI AfaI AluI ApaI
BanI BanII BbvI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
MaeIII MnlI MspI MvaI MwoI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI