Restriction map for
pFN21AE5407
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsiEI BsiWI BspDI BspLU11I
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MscI MseI MspA1I MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 654 TCCGGA T^CCGGA
AcsI 658 GAATTC [GA]^AATT[CT]
AlwI 591 GATCC GGATC(4/5)
ApoI 658 GAATTC [GA]^AATT[CT]
BbrPI 463 CACGTG CAC^GTG
BciVI 81 GGATAC GTATCC(6/5)
BfaI 793 CTAG C^TAG
BsaAI 463 CACGTG [CT]AC^GT[GA]
BsaI 109 GGTCTC GGTCTC(1/5)
BsaWI 654 TCCGGA [AT]^CCGG[AT]
BseAI 654 TCCGGA T^CCGGA
BseRI 343 CTCCTC GAGGAG(10/8)
BsmBI 688 GAGACG CGTCTC(1/5)
Bsp1407I 356 TGTACA T^GTACA
BspEI 654 TCCGGA T^CCGGA
BspHI 717 TCATGA T^CATGA
BspMI 74 ACCTGC ACCTGC(4/8)
BsrBI 131 CCGCTC CCGCTC(-3/-3)
BsrGI 356 TGTACA T^GTACA
BstXI 297 CCAGGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 591 GATC ^GATC
DsaI 167 CCATGG C^C[GA][CT]GG
EcoNI 663 CCTCTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 658 GAATTC G^AATTC
EcoT22I 682 ATGCAT ATGCA^T
HgaI 690 GACGC GACGC(5/10)
HinfI 943 GACTC G^A[ACGT]TC
MaeI 793 CTAG C^TAG
MaeII 464 ACGT A^CGT
MaeIII 466 GTGAC ^GT[ACGT]AC
MboI 591 GATC ^GATC
MroI 654 TCCGGA T^CCGGA
NcoI 167 CCATGG C^CATGG
NdeII 591 GATC ^GATC
NsiI 682 ATGCAT ATGCA^T
NspI 680 GCATGC [GA]CATG^[CT]
PflMI 231 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 943 GACTC GAGTC(4/5)
PmaCI 463 CACGTG CAC^GTG
PmlI 463 CACGTG CAC^GTG
PstI 872 CTGCAG CTGCA^G
RcaI 717 TCATGA T^CATGA
SacI 957 GAGCTC GAGCT^C
SapI 885 GAAGAGC GCTCTTC(1/4)
Sau3AI 591 GATC ^GATC
ScaI 309 AGTACT AGT^ACT
SphI 680 GCATGC GCATG^C
SspBI 356 TGTACA T^GTACA
TaqI 545 TCGA T^CGA
Tsp45I 466 GTGAC ^GT[GC]AC
Tsp509I 659 AATT ^AATT
TthHB8I 545 TCGA T^CGA
Van91I 231 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AocI 807 CCTGAGG CC^T[ACGT]AGG
862 CCTCAGG
ApaI 162 GGGCCC GGGCC^C
951 GGGCCC
AspHI 316 GTGCTC G[AT]GC[AT]^C
957 GAGCTC
BbsI 447 GAAGAC GAAGAC(2/6)
552 GAAGAC
BglI 51 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
395 GCCTCCTGGGC
BpuAI 447 GAAGAC GAAGAC(2/6)
552 GAAGAC
BsgI 753 CTGCAC GTGCAG(16/14)
673 GTGCAG
BsiHKAI 316 GTGCTC G[AT]GC[AT]^C
957 GAGCTC
BsmAI 688 GAGAC GTCTC(1/5)
110 GTCTC
BsmI 253 GCATTC GAATGC(1/-1)
488 GCATTC
BstAPI 629 GCATCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
675 GCAGTGCATGC
Bsu36I 807 CCTGAGG CC^T[ACGT]AGG
862 CCTCAGG
CfoI 768 GCGC GCG^C
774 GCGC
EaeI 494 TGGCCG [CT]^GGCC[GA]
575 TGGCCG
EarI 181 GAAGAG CTCTTC(1/4)
885 GAAGAG
Eco81I 807 CCTGAGG CC^T[ACGT]AGG
862 CCTCAGG
HhaI 768 GCGC GCG^C
774 GCGC
HinP1I 768 GCGC G^CGC
774 GCGC
HphI 528 GGTGA GGTGA(8/7)
347 TCACC
Ksp632I 181 GAAGAG CTCTTC(1/4)
885 GAAGAG
PpuMI 811 AGGACCC [GA]G^G[AT]CC[CT]
821 GGGACCT
SfcI 410 CTACAG C^T[GA][CT]AG
872 CTGCAG
SmaI 54 CCCGGG CCC^GGG
897 CCCGGG
SmlI 265 CTCAAG C^T[CT][GA]AG
891 CTTGAG
StuI 563 AGGCCT AGG^CCT
585 AGGCCT
XcmI 261 CCATCTCAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
470 CCAGAGAGAAGCTGG
XmaCI 54 CCCGGG C^CCGGG
897 CCCGGG
XmaI 54 CCCGGG C^CCGGG
897 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboII MnlI MslI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
StyI TseI TspRI