Restriction map for
pFN21AE4571
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII BbrPI
BbsI BbvCI BciVI BfrI BpuAI
BsaBI BseAI BsiHKAI BsiWI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FseI
FspI HincII HindII HpaI KpnI
MamI MfeI MluI MroI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflIII 1217 ACGTGT A^C[GA][CT]GT
Aor51HI 1856 AGCGCT AGC^GCT
AvrII 381 CCTAGG C^CTAGG
BamHI 899 GGATCC G^GATCC
BanI 1790 GGCGCC G^G[CT][GA]CC
BbeI 1790 GGCGCC GGCGC^C
BclI 1427 TGATCA T^GATCA
BglII 1577 AGATCT A^GATCT
BlnI 381 CCTAGG C^CTAGG
BsaAI 1618 TACGTG [CT]AC^GT[GA]
BsaWI 1829 TCCGGT [AT]^CCGG[AT]
BsiEI 1815 CGACCG CG[GA][CT]^CG
BsmBI 1438 GAGACG CGTCTC(1/5)
BsmI 1402 GCATTC GAATGC(1/-1)
Bsp1407I 1301 TGTACA T^GTACA
BsrFI 1162 GCCGGC [GA]^CCGG[CT]
BsrGI 1301 TGTACA T^GTACA
BssSI 1348 CACGAG CACGAG(-5/-1)
BstAPI 1347 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 735 GGTGACC G^GT[ACGT]ACC
BstPI 735 GGTGACC G^GT[ACGT]ACC
BstXI 1362 CCATGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1162 GCCGGC [GA]^CCGG[CT]
Eco47III 1856 AGCGCT AGC^GCT
EcoO65I 735 GGTGACC G^GT[ACGT]ACC
EcoRI 712 GAATTC G^AATTC
FbaI 1427 TGATCA T^GATCA
HgaI 2071 GCGTC GACGC(5/10)
HindIII 1267 AAGCTT A^AGCTT
KasI 1790 GGCGCC G^GCGCC
KspI 287 CCGCGG CCGC^GG
MseI 1211 TTAA T^TAA
NaeI 1162 GCCGGC GCC^GGC
NarI 1790 GGCGCC GG^CGCC
NdeI 1702 CATATG CA^TATG
NgoMIV 1162 GCCGGC G^CCGGC
NspI 231 ACATGC [GA]CATG^[CT]
SacII 287 CCGCGG CCGC^GG
SfoI 1790 GGCGCC GGC^GCC
SspBI 1301 TGTACA T^GTACA
Tru9I 1211 TTAA T^TAA
XbaI 1851 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 712 GAATTC [GA]^AATT[CT]
979 GAATTT
AcyI 1790 GGCGCC G[GA]^CG[CT]C
2070 GGCGTC
ApoI 712 GAATTC [GA]^AATT[CT]
979 GAATTT
BalI 2153 TGGCCA TGG^CCA
2202 TGGCCA
BcgI 1923 CGAGGGGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1373 GCAAGCTCTTCG
BglI 541 GCCAAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2033 GCCTTGAAGGC
BlpI 186 GCTCAGC GC^T[ACGT]AGC
397 GCTCAGC
Bpu1102I 186 GCTCAGC GC^T[ACGT]AGC
397 GCTCAGC
BsaHI 1790 GGCGCC G[GA]^CG[CT]C
2070 GGCGTC
BsaI 1477 GAGACC GGTCTC(1/5)
1543 GGTCTC
BspMI 436 ACCTGC ACCTGC(4/8)
1335 GCAGGT
BsrBI 1030 GAGCGG CCGCTC(-3/-3)
1470 GAGCGG
CelII 186 GCTCAGC GC^T[ACGT]AGC
397 GCTCAGC
Hin1I 1790 GGCGCC G[GA]^CG[CT]C
2070 GGCGTC
MluNI 2153 TGGCCA TGG^CCA
2202 TGGCCA
MscI 2153 TGGCCA TGG^CCA
2202 TGGCCA
NcoI 530 CCATGG C^CATGG
1362 CCATGG
PflMI 765 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2113 CCAGAGGATGG
PpuMI 209 AGGACCC [GA]G^G[AT]CC[CT]
1295 AGGACCT
SmaI 740 CCCGGG CCC^GGG
1802 CCCGGG
Van91I 765 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2113 CCAGAGGATGG
XcmI 356 CCAAGGAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1889 CCACAGGGGATGTGG
XmaCI 740 CCCGGG C^CCGGG
1802 CCCGGG
XmaI 740 CCCGGG C^CCGGG
1802 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII