Restriction map for
pFN21AE6124
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AfaI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaII
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MluNI MscI MseI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 28 GTCGAC GT^[AC][GT]AC
AccIII 274 TCCGGA T^CCGGA
AhdI 226 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 226 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 53 CCCGAG C^[CT]CG[GA]G
BamHI 79 GGATCC G^GATCC
BanI 199 GGTGCC G^G[CT][GA]CC
BbsI 223 GAAGAC GAAGAC(2/6)
BfaI 209 CTAG C^TAG
BglII 288 AGATCT A^GATCT
BmyI 385 GTGCCC G[AGT]GC[ACT]^C
BpmI 68 CTCCAG CTGGAG(16/14)
BpuAI 223 GAAGAC GAAGAC(2/6)
BsaWI 274 TCCGGA [AT]^CCGG[AT]
BseAI 274 TCCGGA T^CCGGA
BseRI 258 CTCCTC GAGGAG(10/8)
BsmAI 278 GAGAC GTCTC(1/5)
BsmFI 157 GGGAC GGGAC(10/14)
BsoBI 53 CCCGAG C^[CT]CG[GA]G
Bsp1286I 385 GTGCCC G[AGT]GC[ACT]^C
BspEI 274 TCCGGA T^CCGGA
BspHI 312 TCATGA T^CATGA
BspMI 196 GCAGGT ACCTGC(4/8)
BsrI 409 CCAGT ACTGG(1/-1)
BstF5I 416 CATCC GGATG(2/0)
BstXI 389 CCAGCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 379 GCGC GCG^C
DdeI 285 CTTAG C^T[ACGT]AG
Eam1105I 226 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 358 GAAGAG CTCTTC(1/4)
FokI 416 CATCC GGATG(9/13)
HhaI 379 GCGC GCG^C
HinP1I 379 GCGC G^CGC
HincII 28 GTCGAC GT[CT]^[GA]AC
HindII 28 GTCGAC GT[CT]^[GA]AC
HindIII 176 AAGCTT A^AGCTT
HphI 304 GGTGA GGTGA(8/7)
Ksp632I 358 GAAGAG CTCTTC(1/4)
MaeI 209 CTAG C^TAG
MroI 274 TCCGGA T^CCGGA
MslI 313 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 313 CATG CATG^
PflMI 70 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 244 CTGCAG CTGCA^G
RcaI 312 TCATGA T^CATGA
SalI 28 GTCGAC G^TCGAC
SfcI 244 CTGCAG C^T[GA][CT]AG
TaqI 29 TCGA T^CGA
Tsp509I 85 AATT ^AATT
TspRI 410 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 29 TCGA T^CGA
Van91I 70 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 446 CCAGCCTCGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 63 GCGG CCGC(-3/-1)
437 GCGG
AlwI 80 GATCC GGATC(4/5)
79 GGATC
BbvI 341 GCAGC GCAGC(8/12)
376 GCTGC
BcnI 393 CCCGG CC^[GC]GG
402 CCCGG
BstYI 79 GGATCC [GA]^GATC[CT]
288 AGATCT
Cac8I 268 GCAAGC GC[ACGT]^[ACGT]GC
372 GCCTGC
DpnII 80 GATC ^GATC
289 GATC
HinfI 26 GAGTC G^A[ACGT]TC
232 GAGTC
MboI 80 GATC ^GATC
289 GATC
MflI 79 GGATCC [GA]^GATC[CT]
288 AGATCT
NciI 393 CCCGG CC^[GC]GG
402 CCCGG
NdeII 80 GATC ^GATC
289 GATC
PleI 26 GAGTC GAGTC(4/5)
232 GAGTC
Sau3AI 80 GATC ^GATC
289 GATC
TseI 341 GCAGC G^C[AT]GC
376 GCTGC
XhoII 79 GGATCC [GA]^GATC[CT]
288 AGATCT
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BssKI
BstNI Cfr13I EcoRII Fnu4HI HaeIII
HapII HpaII ItaI MboII MnlI
MspI MvaI MwoI NlaIV Sau96I
ScrFI