Restriction map for
pFN21AE4439
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AspI AviII AvrII
BbeI BbrPI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsiEI BsmBI BsmI Bsp1407I
BspDI BspEI BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HaeII HincII HindII
HpaI KasI KpnI Ksp632I KspI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PvuI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AclI 974 AACGTT AA^CGTT
AfaI 328 GTAC GT^AC
AflIII 131 ACATGT A^C[GA][CT]GT
AhdI 3 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 19 CCTCAGG CC^T[ACGT]AGG
ApaI 1252 GGGCCC GGGCC^C
AseI 448 ATTAAT AT^TAAT
AsnI 448 ATTAAT AT^TAAT
Asp700I 87 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 727 CCCGGG C^[CT]CG[GA]G
BanI 460 GGTGCC G^G[CT][GA]CC
BanII 1252 GGGCCC G[GA]GC[CT]^C
BbsI 1165 GTCTTC GAAGAC(2/6)
BbvCI 1273 GCTGAGG CCTCAGC(-5/-2)
BfaI 544 CTAG C^TAG
BpmI 797 CTGGAG CTGGAG(16/14)
BpuAI 1165 GTCTTC GAAGAC(2/6)
BsaBI 432 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 769 GAGGAG GAGGAG(10/8)
BsgI 394 GTGCAG GTGCAG(16/14)
BsiWI 327 CGTACG C^GTACG
BsmFI 135 GTCCC GGGAC(10/14)
BsoBI 727 CCCGGG C^[CT]CG[GA]G
BspLU11I 131 ACATGT A^CATGT
BsrBI 214 GAGCGG CCGCTC(-3/-3)
BstXI 353 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 19 CCTCAGG CC^T[ACGT]AGG
CfoI 578 GCGC GCG^C
Eam1105I 3 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 19 CCTCAGG CC^T[ACGT]AGG
EcoNI 384 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 688 GAATTC G^AATTC
EcoT14I 149 CCTTGG C^C[AT][AT]GG
HhaI 578 GCGC GCG^C
HinP1I 578 GCGC G^CGC
HindIII 715 AAGCTT A^AGCTT
HinfI 793 GAATC G^A[ACGT]TC
MaeI 544 CTAG C^TAG
MaeII 975 ACGT A^CGT
MamI 432 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1361 CAATTG C^AATTG
MunI 1361 CAATTG C^AATTG
PpuMI 723 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1158 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 448 ATTAAT AT^TAAT
Psp1406I 974 AACGTT AA^CGTT
RsaI 328 GTAC GT^AC
SbfI 468 CCTGCAGG CCTGCA^GG
SmaI 727 CCCGGG CCC^GGG
SmlI 712 CTCAAG C^T[CT][GA]AG
Sse8387I 468 CCTGCAGG CCTGCA^GG
StyI 149 CCTTGG C^C[AT][AT]GG
TaqI 1169 TCGA T^CGA
TfiI 793 GAATC G^A[AT]TC
TthHB8I 1169 TCGA T^CGA
XmaCI 727 CCCGGG C^CCGGG
XmaI 727 CCCGGG C^CCGGG
XmnI 87 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 87 GAATTT [GA]^AATT[CT]
688 GAATTC
AcyI 175 GACGCC G[GA]^CG[CT]C
183 GGCGTC
ApoI 87 GAATTT [GA]^AATT[CT]
688 GAATTC
AspHI 442 GTGCTC G[AT]GC[AT]^C
1171 GAGCAC
AvaII 724 GGACC G^G[AT]CC
750 GGACC
BamHI 314 GGATCC G^GATCC
332 GGATCC
BcgI 1170 CGAGCACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1241 GCATGTGCTTCG
BclI 371 TGATCA T^GATCA
431 TGATCA
BsaHI 175 GACGCC G[GA]^CG[CT]C
183 GGCGTC
BsaI 739 GAGACC GGTCTC(1/5)
1282 GAGACC
BsiHKAI 442 GTGCTC G[AT]GC[AT]^C
1171 GAGCAC
BstYI 314 GGATCC [GA]^GATC[CT]
332 GGATCC
DraII 539 AGGGCCT [GA]G^G[ACGT]CC[CT]
723 AGGACCC
DsaI 82 CCGTGG C^C[GA][CT]GG
563 CCGTGG
EcoO109I 539 AGGGCCT [GA]G^G[ACGT]CC[CT]
723 AGGACCC
FauI 680 CCCGC CCCGC(4/6)
1145 CCCGC
FbaI 371 TGATCA T^GATCA
431 TGATCA
HgaI 175 GACGC GACGC(5/10)
184 GCGTC
Hin1I 175 GACGCC G[GA]^CG[CT]C
183 GGCGTC
MflI 314 GGATCC [GA]^GATC[CT]
332 GGATCC
MseI 449 TTAA T^TAA
1139 TTAA
NspI 131 ACATGT [GA]CATG^[CT]
1241 GCATGT
PstI 197 CTGCAG CTGCA^G
469 CTGCAG
SfcI 197 CTGCAG C^T[GA][CT]AG
469 CTGCAG
Tru9I 449 TTAA T^TAA
1139 TTAA
XhoII 314 GGATCC [GA]^GATC[CT]
332 GGATCC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AlwNI BalI
BbvI BcnI BglI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII RcaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
Tsp509I TspRI