Restriction map for
pFN21AE4407
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Van91I XbaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AcsI 350 GAATTC [GA]^AATT[CT]
AcyI 1237 GGCGCC G[GA]^CG[CT]C
AlwNI 913 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 68 CCTCAGG CC^T[ACGT]AGG
ApaI 890 GGGCCC GGGCC^C
ApoI 350 GAATTC [GA]^AATT[CT]
Asp700I 546 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 591 GAGCTC G[AT]GC[AT]^C
AspI 709 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 315 GGACC G^G[AT]CC
BbeI 1237 GGCGCC GGCGC^C
BbsI 622 GTCTTC GAAGAC(2/6)
BfaI 139 CTAG C^TAG
BglII 44 AGATCT A^GATCT
BlpI 1098 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1098 GCTAAGC GC^T[ACGT]AGC
BpuAI 622 GTCTTC GAAGAC(2/6)
BsaHI 1237 GGCGCC G[GA]^CG[CT]C
BsiHKAI 591 GAGCTC G[AT]GC[AT]^C
BspMI 965 ACCTGC ACCTGC(4/8)
BsrBI 559 CCGCTC CCGCTC(-3/-3)
BsrFI 613 GCCGGC [GA]^CCGG[CT]
BstAPI 912 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 714 GGTCACC G^GT[ACGT]ACC
BstPI 714 GGTCACC G^GT[ACGT]ACC
BstXI 1154 CCAAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 44 AGATCT [GA]^GATC[CT]
Bsu36I 68 CCTCAGG CC^T[ACGT]AGG
CelII 1098 GCTAAGC GC^T[ACGT]AGC
Cfr10I 613 GCCGGC [GA]^CCGG[CT]
DsaI 970 CCGCGG C^C[GA][CT]GG
Eco81I 68 CCTCAGG CC^T[ACGT]AGG
EcoO65I 714 GGTCACC G^GT[ACGT]ACC
EcoRI 350 GAATTC G^AATTC
FauI 972 GCGGG CCCGC(4/6)
Hin1I 1237 GGCGCC G[GA]^CG[CT]C
HinfI 1050 GATTC G^A[ACGT]TC
KasI 1237 GGCGCC G^GCGCC
KspI 970 CCGCGG CCGC^GG
MaeI 139 CTAG C^TAG
MaeII 1013 ACGT A^CGT
MflI 44 AGATCT [GA]^GATC[CT]
MslI 231 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 613 GCCGGC GCC^GGC
NarI 1237 GGCGCC GG^CGCC
NgoMIV 613 GCCGGC G^CCGGC
NlaIII 1161 CATG CATG^
PaeR7I 275 CTCGAG C^TCGAG
PflFI 709 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 314 AGGACCT [GA]G^G[AT]CC[CT]
SacI 591 GAGCTC GAGCT^C
SacII 970 CCGCGG CCGC^GG
SexAI 428 ACCAGGT A^CC[AT]GGT
SfaNI 1140 GATGC GCATC(5/9)
SfoI 1237 GGCGCC GGC^GCC
TfiI 1050 GATTC G^A[AT]TC
Tth111I 709 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 695 CCAGGGAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 275 CTCGAG C^TCGAG
XhoII 44 AGATCT [GA]^GATC[CT]
XmnI 546 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 671 GACAATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
943 GACAAGCTGTC
AspEI 671 GACAATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
943 GACAAGCTGTC
BalI 128 TGGCCA TGG^CCA
1151 TGGCCA
BanI 229 GGCACC G^G[CT][GA]CC
1237 GGCGCC
BpmI 391 CTGGAG CTGGAG(16/14)
541 CTGGAG
BsaI 598 GAGACC GGTCTC(1/5)
732 GAGACC
BsmAI 598 GAGAC GTCTC(1/5)
732 GAGAC
BsmFI 341 GGGAC GGGAC(10/14)
679 GTCCC
BsrDI 751 CATTGC GCAATG(2/0)
1127 GCAATG
DpnII 45 GATC ^GATC
261 GATC
DraII 314 AGGACCT [GA]G^G[ACGT]CC[CT]
889 GGGGCCC
Eam1105I 671 GACAATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
943 GACAAGCTGTC
EcoO109I 314 AGGACCT [GA]G^G[ACGT]CC[CT]
889 GGGGCCC
HgaI 150 GACGC GACGC(5/10)
250 GACGC
MboI 45 GATC ^GATC
261 GATC
MluNI 128 TGGCCA TGG^CCA
1151 TGGCCA
MscI 128 TGGCCA TGG^CCA
1151 TGGCCA
MseI 820 TTAA T^TAA
904 TTAA
NdeII 45 GATC ^GATC
261 GATC
PstI 94 CTGCAG CTGCA^G
919 CTGCAG
PvuII 502 CAGCTG CAG^CTG
1007 CAGCTG
Sau3AI 45 GATC ^GATC
261 GATC
SfcI 94 CTGCAG C^T[GA][CT]AG
919 CTGCAG
SmaI 787 CCCGGG CCC^GGG
893 CCCGGG
SmlI 275 CTCGAG C^T[CT][GA]AG
481 CTCAAG
StuI 650 AGGCCT AGG^CCT
839 AGGCCT
TaqI 276 TCGA T^CGA
308 TCGA
Tru9I 820 TTAA T^TAA
904 TTAA
Tsp509I 351 AATT ^AATT
674 AATT
TthHB8I 276 TCGA T^CGA
308 TCGA
XmaCI 787 CCCGGG C^CCGGG
893 CCCGGG
XmaI 787 CCCGGG C^CCGGG
893 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanII BbvI BcnI BmyI BsaJI
BseRI BsgI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
RsaI SapI Sau96I ScrFI StyI
TseI Tsp45I TspRI