Restriction map for
pFN21AE9695
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# NO CUT ENZYMES ( 98 enzymes) #
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Acc65I AccIII AclI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BlnI
BsaBI BsaI BseAI BsmI BspDI
BspEI BspLU11I BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoT22I FbaI FseI FspI
HpaI KasI KpnI KspI MaeI
MamI MfeI MroI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI TfiI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1566 GACGTC GACGT^C
AccI 766 GTCTAC GT^[AC][GT]AC
AcsI 287 AAATTC [GA]^AATT[CT]
AflII 1867 CTTAAG C^TTAAG
Alw44I 1665 GTGCAC G^TGCAC
ApaI 694 GGGCCC GGGCC^C
ApaLI 1665 GTGCAC G^TGCAC
ApoI 287 AAATTC [GA]^AATT[CT]
BanI 1287 GGTGCC G^G[CT][GA]CC
BbsI 418 GTCTTC GAAGAC(2/6)
BfrI 1867 CTTAAG C^TTAAG
BglII 1151 AGATCT A^GATCT
BlpI 1348 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1348 GCTGAGC GC^T[ACGT]AGC
BpuAI 418 GTCTTC GAAGAC(2/6)
BsaAI 892 TACGTG [CT]AC^GT[GA]
BsaWI 458 TCCGGT [AT]^CCGG[AT]
BsiWI 344 CGTACG C^GTACG
BsmBI 882 CGTCTC CGTCTC(1/5)
Bsp1407I 737 TGTACA T^GTACA
BspHI 167 TCATGA T^CATGA
BsrDI 842 GCAATG GCAATG(2/0)
BsrGI 737 TGTACA T^GTACA
BstAPI 1022 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 15 GGTCACC G^GT[ACGT]ACC
BstPI 15 GGTCACC G^GT[ACGT]ACC
CelII 1348 GCTGAGC GC^T[ACGT]AGC
DrdI 124 GACTGCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 712 CTCTTC CTCTTC(1/4)
EcoO65I 15 GGTCACC G^GT[ACGT]ACC
EcoRV 1816 GATATC GAT^ATC
HindIII 1693 AAGCTT A^AGCTT
Ksp632I 712 CTCTTC CTCTTC(1/4)
MluI 907 ACGCGT A^CGCGT
MseI 1868 TTAA T^TAA
NotI 1910 GCGGCCGC GC^GGCCGC
PshAI 906 GACGCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 167 TCATGA T^CATGA
SacI 226 GAGCTC GAGCT^C
SmaI 935 CCCGGG CCC^GGG
SmlI 1867 CTTAAG C^T[CT][GA]AG
SphI 54 GCATGC GCATG^C
SspBI 737 TGTACA T^GTACA
Tru9I 1868 TTAA T^TAA
Tsp509I 288 AATT ^AATT
XcmI 1259 CCAGCCCTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 935 CCCGGG C^CCGGG
XmaI 935 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 65 ACGTGT A^C[GA][CT]GT
907 ACGCGT
AlwNI 741 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
970 CAGCCACTG
AvaI 220 CCCGAG C^[CT]CG[GA]G
935 CCCGGG
BpmI 652 CTGGAG CTGGAG(16/14)
1297 CTGGAG
BsiEI 491 CGGCCG CG[GA][CT]^CG
1911 CGGCCG
BsmAI 455 GTCTC GTCTC(1/5)
883 GTCTC
BsoBI 220 CCCGAG C^[CT]CG[GA]G
935 CCCGGG
BstXI 5 CCACCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1490 CCATGTGTGTGG
EagI 491 CGGCCG C^GGCCG
1911 CGGCCG
EclXI 491 CGGCCG C^GGCCG
1911 CGGCCG
Eco52I 491 CGGCCG C^GGCCG
1911 CGGCCG
HaeII 988 AGCGCC [GA]GCGC^[CT]
1070 AGCGCC
NspI 54 GCATGC [GA]CATG^[CT]
1784 ACATGC
SfiI 825 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1903 GGCCTGAGCGGCC
StuI 1397 AGGCCT AGG^CCT
1902 AGGCCT
TaqI 584 TCGA T^CGA
680 TCGA
TthHB8I 584 TCGA T^CGA
680 TCGA
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BalI BanII
BbvI BcnI BglI BmyI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XhoII