Restriction map for
pFN21AE3913
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AhdI AlwI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI AspEI AvrII BalI BamHI
BanII BbrPI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BseAI BsgI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstXI BstYI BstZ17I Bsu36I CelII
ClaI DpnII DraI DraIII EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI HindIII
HpaI Ksp632I MaeI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI SacI SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp509I
XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 90 GACGTC GACGT^C
AgeI 326 ACCGGT A^CCGGT
Alw44I 193 GTGCAC G^TGCAC
ApaLI 193 GTGCAC G^TGCAC
Asp700I 345 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 398 TGCGCA TGC^GCA
BbeI 30 GGCGCC GGCGC^C
BbvCI 570 CCTCAGC CCTCAGC(-5/-2)
BsaWI 326 ACCGGT [AT]^CCGG[AT]
BseRI 25 GAGGAG GAGGAG(10/8)
BsiEI 582 CGGTCG CG[GA][CT]^CG
BsmFI 209 GGGAC GGGAC(10/14)
BsrBI 154 GAGCGG CCGCTC(-3/-3)
BsrFI 326 ACCGGT [GA]^CCGG[CT]
BsrI 414 ACTGG ACTGG(1/-1)
BstEII 624 GGTGACC G^GT[ACGT]ACC
BstPI 624 GGTGACC G^GT[ACGT]ACC
Cfr10I 326 ACCGGT [GA]^CCGG[CT]
CpoI 46 CGGTCCG CG^G[AT]CCG
DdeI 571 CTCAG C^T[ACGT]AG
DraII 118 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 262 GACGCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 118 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 624 GGTGACC G^GT[ACGT]ACC
FspI 398 TGCGCA TGC^GCA
HaeII 30 GGCGCC [GA]GCGC^[CT]
HapII 327 CCGG C^CGG
HincII 166 GTCAAC GT[CT]^[GA]AC
HindII 166 GTCAAC GT[CT]^[GA]AC
HinfI 345 GAATC G^A[ACGT]TC
HpaII 327 CCGG C^CGG
KasI 30 GGCGCC G^GCGCC
MseI 323 TTAA T^TAA
MslI 372 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 327 CCGG C^CGG
NarI 30 GGCGCC GG^CGCC
PaeR7I 505 CTCGAG C^TCGAG
PflFI 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 468 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 326 ACCGGT A^CCGGT
RsrII 46 CGGTCCG CG^G[AT]CCG
SfoI 30 GGCGCC GGC^GCC
SmlI 505 CTCGAG C^T[CT][GA]AG
TfiI 345 GAATC G^A[AT]TC
Tru9I 323 TTAA T^TAA
TspRI 413 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 468 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 368 CCATCATCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 505 CTCGAG C^TCGAG
XmnI 345 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 332 GGTACC G^GTACC
363 GGTACC
Asp718I 332 GGTACC G^GTACC
363 GGTACC
AspHI 193 GTGCAC G[AT]GC[AT]^C
510 GTGCTC
AvaI 127 CCCGAG C^[CT]CG[GA]G
505 CTCGAG
BglI 112 GCCGATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
559 GCCGAGGTGGC
BmyI 193 GTGCAC G[AGT]GC[ACT]^C
510 GTGCTC
BsiHKAI 193 GTGCAC G[AT]GC[AT]^C
510 GTGCTC
BsoBI 127 CCCGAG C^[CT]CG[GA]G
505 CTCGAG
Bsp1286I 193 GTGCAC G[AGT]GC[ACT]^C
510 GTGCTC
BspMI 170 ACCTGC ACCTGC(4/8)
395 ACCTGC
EcoNI 396 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
455 CCTACCACAGG
FauI 19 GCGGG CCCGC(4/6)
83 GCGGG
HgaI 262 GACGC GACGC(5/10)
269 GCGTC
KpnI 332 GGTACC GGTAC^C
363 GGTACC
KspI 17 CCGCGG CCGC^GG
579 CCGCGG
MaeII 91 ACGT A^CGT
533 ACGT
MspA1I 17 CCGCGG C[AC]G^C[GT]G
579 CCGCGG
NlaIII 162 CATG CATG^
526 CATG
SacII 17 CCGCGG CCGC^GG
579 CCGCGG
TaqI 287 TCGA T^CGA
506 TCGA
TthHB8I 287 TCGA T^CGA
506 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BbsI BbvI BpuAI
BsaHI BsaJI BsiYI BslI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DsaI EcoRII Fnu4HI HaeIII HhaI
Hin1I HinP1I HphI ItaI MaeIII
MboII MnlI MvaI MvnI MwoI
NlaIV RsaI Sau96I ScrFI TseI
Tsp45I