Restriction map for
pFN21AE1527
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BcgI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 403 GTAGAC GT^[AC][GT]AC
AfaI 333 GTAC GT^AC
AflIII 524 ACACGT A^C[GA][CT]GT
AocI 241 CCTGAGG CC^T[ACGT]AGG
AvaI 185 CCCGGG C^[CT]CG[GA]G
AvaII 51 GGACC G^G[AT]CC
BbvCI 588 CCTCAGC CCTCAGC(-5/-2)
BciVI 439 GTATCC GTATCC(6/5)
BglII 548 AGATCT A^GATCT
BmyI 608 GTGCCC G[AGT]GC[ACT]^C
BpmI 89 CTCCAG CTGGAG(16/14)
BsaAI 436 TACGTA [CT]AC^GT[GA]
BsgI 250 GTGCAG GTGCAG(16/14)
BsoBI 185 CCCGGG C^[CT]CG[GA]G
Bsp1286I 608 GTGCCC G[AGT]GC[ACT]^C
BspHI 75 TCATGA T^CATGA
BsrBI 160 GAGCGG CCGCTC(-3/-3)
BsrFI 53 ACCGGC [GA]^CCGG[CT]
BsrI 71 CCAGT ACTGG(1/-1)
BssHII 168 GCGCGC G^CGCGC
BstAPI 107 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 444 CATCC GGATG(2/0)
BstYI 548 AGATCT [GA]^GATC[CT]
Bsu36I 241 CCTGAGG CC^T[ACGT]AGG
Cfr10I 53 ACCGGC [GA]^CCGG[CT]
CpoI 50 CGGACCG CG^G[AT]CCG
EarI 352 CTCTTC CTCTTC(1/4)
Eco81I 241 CCTGAGG CC^T[ACGT]AGG
EcoT14I 455 CCAAGG C^C[AT][AT]GG
FokI 444 CATCC GGATG(9/13)
HphI 462 GGTGA GGTGA(8/7)
Ksp632I 352 CTCTTC CTCTTC(1/4)
MflI 548 AGATCT [GA]^GATC[CT]
MslI 555 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 393 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 75 TCATGA T^CATGA
RsaI 333 GTAC GT^AC
RsrII 50 CGGACCG CG^G[AT]CCG
SapI 351 GCTCTTC GCTCTTC(1/4)
SmaI 185 CCCGGG CCC^GGG
SnaBI 436 TACGTA TAC^GTA
StuI 179 AGGCCT AGG^CCT
StyI 455 CCAAGG C^C[AT][AT]GG
TaqI 530 TCGA T^CGA
TthHB8I 530 TCGA T^CGA
Van91I 393 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 389 CCAACCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 548 AGATCT [GA]^GATC[CT]
XmaCI 185 CCCGGG C^CCGGG
XmaI 185 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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BstNI 149 CCTGG CC^[AT]GG
468 CCTGG
DpnII 30 GATC ^GATC
549 GATC
EcoRII 149 CCTGG ^CC[AT]GG
468 CCTGG
HgaI 299 GACGC GACGC(5/10)
535 GACGC
HinfI 472 GACTC G^A[ACGT]TC
571 GACTC
MaeII 437 ACGT A^CGT
526 ACGT
MboI 30 GATC ^GATC
549 GATC
MboII 501 GAAGA GAAGA(8/7)
353 TCTTC
MvaI 149 CCTGG CC^[AT]GG
468 CCTGG
NdeII 30 GATC ^GATC
549 GATC
NspI 347 ACATGC [GA]CATG^[CT]
396 GCATGT
PleI 472 GACTC GAGTC(4/5)
571 GACTC
PvuII 213 CAGCTG CAG^CTG
541 CAGCTG
Sau3AI 30 GATC ^GATC
549 GATC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BseRI BsiYI BslI BsmFI
BssKI BstUI Cac8I CfoI Cfr13I
DdeI FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspA1I MspI MvnI MwoI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfaNI SfcI TseI