Restriction map for
pFN21AE4425
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspI
AvaII AviII BalI BbeI BbrPI
BbsI BbvCI BclI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1064 GGTACC G^GTACC
AccII 1041 CGCG CG^CG
AflIII 868 ACGTGT A^C[GA][CT]GT
AhdI 778 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 791 GGGCCC GGGCC^C
Asp718I 1064 GGTACC G^GTACC
AspEI 778 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 264 CCTAGG C^CTAGG
BamHI 313 GGATCC G^GATCC
BcgI 849 CGAGGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 433 GGATAC GTATCC(6/5)
BlnI 264 CCTAGG C^CTAGG
BsaBI 250 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1036 GAGACC GGTCTC(1/5)
BseRI 874 GAGGAG GAGGAG(10/8)
BsgI 236 GTGCAG GTGCAG(16/14)
BsrBI 426 GAGCGG CCGCTC(-3/-3)
BsrDI 855 CATTGC GCAATG(2/0)
BssSI 400 CTCGTG CACGAG(-5/-1)
BstNI 9 CCTGG CC^[AT]GG
BstUI 1041 CGCG CG^CG
DraII 791 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1076 TGGCCG [CT]^GGCC[GA]
Eam1105I 778 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1189 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 791 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 145 GAATTC G^AATTC
EcoRII 9 CCTGG ^CC[AT]GG
HapII 789 CCGG C^CGG
HinfI 739 GAGTC G^A[ACGT]TC
HpaII 789 CCGG C^CGG
KpnI 1064 GGTACC GGTAC^C
MaeII 868 ACGT A^CGT
MaeIII 167 GTCAC ^GT[ACGT]AC
MamI 250 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 185 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 653 CAGCTG C[AC]G^C[GT]G
MspI 789 CCGG C^CGG
MvaI 9 CCTGG CC^[AT]GG
MvnI 1041 CGCG CG^CG
PflMI 157 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 739 GAGTC GAGTC(4/5)
PstI 929 CTGCAG CTGCA^G
PvuII 653 CAGCTG CAG^CTG
SacI 1159 GAGCTC GAGCT^C
SmaI 788 CCCGGG CCC^GGG
Tsp45I 167 GTCAC ^GT[GC]AC
Van91I 157 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 788 CCCGGG C^CCGGG
XmaI 788 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 32 GTAC GT^AC
1065 GTAC
Asp700I 112 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
952 GAAGTTCTTC
AvaI 415 CTCGGG C^[CT]CG[GA]G
788 CCCGGG
BanI 1064 GGTACC G^G[CT][GA]CC
1185 GGCACC
BcnI 788 CCCGG CC^[GC]GG
789 CCGGG
BpmI 923 CTGGAG CTGGAG(16/14)
1016 CTGGAG
BsmAI 1036 GAGAC GTCTC(1/5)
741 GTCTC
BsoBI 415 CTCGGG C^[CT]CG[GA]G
788 CCCGGG
BstAPI 202 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
238 GCAGTTGATGC
BstYI 313 GGATCC [GA]^GATC[CT]
772 AGATCC
CfoI 379 GCGC GCG^C
393 GCGC
Cfr13I 791 GGGCC G^G[ACGT]CC
792 GGCCC
FauI 1069 CCCGC CCCGC(4/6)
1199 GCGGG
HhaI 379 GCGC GCG^C
393 GCGC
HinP1I 379 GCGC G^CGC
393 GCGC
HphI 24 GGTGA GGTGA(8/7)
358 GGTGA
MflI 313 GGATCC [GA]^GATC[CT]
772 AGATCC
NciI 788 CCCGG CC^[GC]GG
789 CCGGG
NcoI 157 CCATGG C^CATGG
1073 CCATGG
NlaIII 158 CATG CATG^
1074 CATG
RsaI 32 GTAC GT^AC
1065 GTAC
Sau96I 791 GGGCC G^G[ACGT]CC
792 GGCCC
SmlI 835 CTTGAG C^T[CT][GA]AG
1033 CTTGAG
TaqI 83 TCGA T^CGA
97 TCGA
TthHB8I 83 TCGA T^CGA
97 TCGA
XhoII 313 GGATCC [GA]^GATC[CT]
772 AGATCC
XmnI 112 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
952 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI BanII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
Cac8I DdeI DpnII DsaI EcoT14I
Fnu4HI FokI HaeIII ItaI MaeI
MboI MboII MnlI MseI MwoI
NdeII NlaIV Sau3AI ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp509I
TspRI