Restriction map for
pFN21AE1473
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanII BbeI BbvCI BcgI BclI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI Hin1I HpaI KasI KpnI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflII 244 CTTAAG C^TTAAG
Asp700I 1049 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 784 GAGCAC G[AT]GC[AT]^C
BanI 297 GGTGCC G^G[CT][GA]CC
BbrPI 1086 CACGTG CAC^GTG
BbsI 1070 GTCTTC GAAGAC(2/6)
BciVI 171 GGATAC GTATCC(6/5)
BfrI 244 CTTAAG C^TTAAG
BpuAI 1070 GTCTTC GAAGAC(2/6)
BsaAI 1086 CACGTG [CT]AC^GT[GA]
BseRI 40 CTCCTC GAGGAG(10/8)
BsgI 657 GTGCAG GTGCAG(16/14)
BsiHKAI 784 GAGCAC G[AT]GC[AT]^C
BsmAI 925 GTCTC GTCTC(1/5)
BsmI 844 GAATGC GAATGC(1/-1)
BspHI 1020 TCATGA T^CATGA
BsrFI 87 GCCGGC [GA]^CCGG[CT]
BstF5I 320 CATCC GGATG(2/0)
Cfr10I 87 GCCGGC [GA]^CCGG[CT]
DpnII 237 GATC ^GATC
DraI 444 TTTAAA TTT^AAA
DraII 872 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 872 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 752 ATGCAT ATGCA^T
FauI 25 CCCGC CCCGC(4/6)
FokI 320 CATCC GGATG(9/13)
HaeII 30 GGCGCT [GA]GCGC^[CT]
HgaI 1116 GACGC GACGC(5/10)
HincII 1098 GTTGAC GT[CT]^[GA]AC
HindII 1098 GTTGAC GT[CT]^[GA]AC
HindIII 201 AAGCTT A^AGCTT
KspI 26 CCGCGG CCGC^GG
MboI 237 GATC ^GATC
MspA1I 26 CCGCGG C[AC]G^C[GT]G
NaeI 87 GCCGGC GCC^GGC
NcoI 1127 CCATGG C^CATGG
NdeII 237 GATC ^GATC
NgoMIV 87 GCCGGC G^CCGGC
NsiI 752 ATGCAT ATGCA^T
NspI 754 GCATGT [GA]CATG^[CT]
PleI 229 GACTC GAGTC(4/5)
PmaCI 1086 CACGTG CAC^GTG
PmlI 1086 CACGTG CAC^GTG
PpuMI 872 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1020 TCATGA T^CATGA
SacII 26 CCGCGG CCGC^GG
Sau3AI 237 GATC ^GATC
SexAI 528 ACCAGGT A^CC[AT]GGT
SfaNI 319 GCATC GCATC(5/9)
SfcI 917 CTACAG C^T[GA][CT]AG
TfiI 767 GATTC G^A[AT]TC
XmnI 1049 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 1085 ACACGT A^C[GA][CT]GT
1087 ACGTGT
AvaI 43 CTCGGG C^[CT]CG[GA]G
166 CCCGAG
AvaII 452 GGTCC G^G[AT]CC
873 GGTCC
BcnI 56 CCCGG CC^[GC]GG
496 CCGGG
BfaI 602 CTAG C^TAG
1120 CTAG
BglI 242 GCCTTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
300 GCCAACAAGGC
BsiEI 13 CGGCCG CG[GA][CT]^CG
89 CGGCCG
BsmFI 1114 GGGAC GGGAC(10/14)
874 GTCCC
BsoBI 43 CTCGGG C^[CT]CG[GA]G
166 CCCGAG
BsrI 358 CCAGT ACTGG(1/-1)
633 CCAGT
CfoI 10 GCGC GCG^C
31 GCGC
DsaI 26 CCGCGG C^C[GA][CT]GG
1127 CCATGG
EaeI 13 CGGCCG [CT]^GGCC[GA]
89 CGGCCG
EagI 13 CGGCCG C^GGCCG
89 CGGCCG
EarI 271 GAAGAG CTCTTC(1/4)
401 GAAGAG
EclXI 13 CGGCCG C^GGCCG
89 CGGCCG
Eco52I 13 CGGCCG C^GGCCG
89 CGGCCG
EcoT14I 869 CCAAGG C^C[AT][AT]GG
1127 CCATGG
HhaI 10 GCGC GCG^C
31 GCGC
HinP1I 10 GCGC G^CGC
31 GCGC
HinfI 229 GACTC G^A[ACGT]TC
767 GATTC
Ksp632I 271 GAAGAG CTCTTC(1/4)
401 GAAGAG
MaeI 602 CTAG C^TAG
1120 CTAG
MseI 245 TTAA T^TAA
445 TTAA
MslI 839 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1015 CATTTTCATG
NciI 56 CCCGG CC^[GC]GG
496 CCGGG
SmlI 244 CTTAAG C^T[CT][GA]AG
974 CTCAAG
StyI 869 CCAAGG C^C[AT][AT]GG
1127 CCATGG
TaqI 464 TCGA T^CGA
595 TCGA
Tru9I 245 TTAA T^TAA
445 TTAA
TspRI 233 CAGTG CA[GC]TG[ACGT][ACGT]^
634 CAGTG
TthHB8I 464 TCGA T^CGA
595 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI BstUI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HpaII HphI ItaI
MaeII MaeIII MboII MnlI MspI
MvaI MvnI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI TseI Tsp45I
Tsp509I