Restriction map for
pFN21AE4337
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp45I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 762 CTTAAG C^TTAAG
AocI 968 CCTTAGG CC^T[ACGT]AGG
BalI 740 TGGCCA TGG^CCA
BamHI 597 GGATCC G^GATCC
BanI 568 GGTGCC G^G[CT][GA]CC
BfrI 762 CTTAAG C^TTAAG
BlpI 392 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 392 GCTCAGC GC^T[ACGT]AGC
BsgI 292 GTGCAG GTGCAG(16/14)
BsiEI 647 CGATCG CG[GA][CT]^CG
BsmBI 223 CGTCTC CGTCTC(1/5)
BspDI 94 ATCGAT AT^CGAT
BspHI 353 TCATGA T^CATGA
BspMI 422 ACCTGC ACCTGC(4/8)
BsrBI 171 CCGCTC CCGCTC(-3/-3)
BsrI 417 CCAGT ACTGG(1/-1)
BstXI 573 CCATGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 968 CCTTAGG CC^T[ACGT]AGG
CelII 392 GCTCAGC GC^T[ACGT]AGC
ClaI 94 ATCGAT AT^CGAT
EaeI 740 TGGCCA [CT]^GGCC[GA]
EarI 226 CTCTTC CTCTTC(1/4)
Eco81I 968 CCTTAGG CC^T[ACGT]AGG
EcoT14I 743 CCAAGG C^C[AT][AT]GG
HaeII 1001 GGCGCT [GA]GCGC^[CT]
HgaI 222 GCGTC GACGC(5/10)
HincII 319 GTTAAC GT[CT]^[GA]AC
HindII 319 GTTAAC GT[CT]^[GA]AC
HpaI 319 GTTAAC GTT^AAC
Ksp632I 226 CTCTTC CTCTTC(1/4)
MluNI 740 TGGCCA TGG^CCA
MscI 740 TGGCCA TGG^CCA
PleI 286 GAGTC GAGTC(4/5)
PpuMI 506 GGGACCT [GA]G^G[AT]CC[CT]
PstI 832 CTGCAG CTGCA^G
PvuI 647 CGATCG CGAT^CG
RcaI 353 TCATGA T^CATGA
SexAI 377 ACCTGGT A^CC[AT]GGT
StuI 857 AGGCCT AGG^CCT
StyI 743 CCAAGG C^C[AT][AT]GG
TfiI 1017 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BseRI 187 GAGGAG GAGGAG(10/8)
946 GAGGAG
BsmAI 224 GTCTC GTCTC(1/5)
634 GTCTC
BsmFI 331 GGGAC GGGAC(10/14)
506 GGGAC
BsrDI 753 CATTGC GCAATG(2/0)
680 GCAATG
CfoI 549 GCGC GCG^C
1002 GCGC
DraII 506 GGGACCT [GA]G^G[ACGT]CC[CT]
662 AGGCCCT
DraIII 49 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
364 CACGGGGTG
EcoNI 429 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
510 CCTGTGCCAGG
EcoO109I 506 GGGACCT [GA]G^G[ACGT]CC[CT]
662 AGGCCCT
HhaI 549 GCGC GCG^C
1002 GCGC
HinP1I 549 GCGC G^CGC
1002 GCGC
HinfI 286 GAGTC G^A[ACGT]TC
1017 GAATC
MaeIII 19 GTAAC ^GT[ACGT]AC
920 GTTAC
MseI 320 TTAA T^TAA
763 TTAA
SfcI 217 CTACAG C^T[GA][CT]AG
832 CTGCAG
SmlI 271 CTCAAG C^T[CT][GA]AG
762 CTTAAG
TaqI 95 TCGA T^CGA
491 TCGA
Tru9I 320 TTAA T^TAA
763 TTAA
TthHB8I 95 TCGA T^CGA
491 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
RsaI Sau3AI Sau96I ScrFI TseI
TspRI Van91I XcmI XhoII