Restriction map for
pFN21AE2278
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbeI BbvCI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KasI Ksp632I KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp509I Tth111I
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 409 GGTACC G^GTACC
AccI 55 GTCTAC GT^[AC][GT]AC
AccII 60 CGCG CG^CG
AcyI 244 GGCGTC G[GA]^CG[CT]C
AflIII 569 ACATGT A^C[GA][CT]GT
Aor51HI 89 AGCGCT AGC^GCT
ApaI 821 GGGCCC GGGCC^C
Asp718I 409 GGTACC G^GTACC
AspHI 359 GTGCTC G[AT]GC[AT]^C
AvaI 899 CCCGAG C^[CT]CG[GA]G
BanI 409 GGTACC G^G[CT][GA]CC
BanII 821 GGGCCC G[GA]GC[CT]^C
BbrPI 493 CACGTG CAC^GTG
BcgI 767 GCACACCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 712 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 26 AGATCT A^GATCT
BsaAI 493 CACGTG [CT]AC^GT[GA]
BsaHI 244 GGCGTC G[GA]^CG[CT]C
BsaI 310 GAGACC GGTCTC(1/5)
BsiHKAI 359 GTGCTC G[AT]GC[AT]^C
BsoBI 899 CCCGAG C^[CT]CG[GA]G
BspLU11I 569 ACATGT A^CATGT
BspMI 459 GCAGGT ACCTGC(4/8)
BsrDI 813 CATTGC GCAATG(2/0)
BssSI 481 CTCGTG CACGAG(-5/-1)
BstUI 60 CGCG CG^CG
BstXI 716 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 520 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 89 AGCGCT AGC^GCT
HgaI 245 GCGTC GACGC(5/10)
Hin1I 244 GGCGTC G[GA]^CG[CT]C
KpnI 409 GGTACC GGTAC^C
MaeII 494 ACGT A^CGT
MaeIII 275 GTGAC ^GT[ACGT]AC
MseI 784 TTAA T^TAA
MslI 717 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 60 CGCG CG^CG
PmaCI 493 CACGTG CAC^GTG
PmlI 493 CACGTG CAC^GTG
PstI 851 CTGCAG CTGCA^G
SfcI 851 CTGCAG C^T[GA][CT]AG
SfiI 711 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 414 CTCAAG C^T[CT][GA]AG
TaqI 795 TCGA T^CGA
Tru9I 784 TTAA T^TAA
Tsp45I 275 GTGAC ^GT[GC]AC
TthHB8I 795 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 126 GTAC GT^AC
410 GTAC
AvaII 696 GGACC G^G[AT]CC
912 GGACC
BamHI 227 GGATCC G^GATCC
393 GGATCC
BbsI 498 GAAGAC GAAGAC(2/6)
649 GAAGAC
BbvI 110 GCAGC GCAGC(8/12)
853 GCAGC
BfaI 625 CTAG C^TAG
748 CTAG
BmyI 359 GTGCTC G[AGT]GC[ACT]^C
821 GGGCCC
BpuAI 498 GAAGAC GAAGAC(2/6)
649 GAAGAC
BsaBI 394 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
984 GATCGCCATC
BseRI 121 GAGGAG GAGGAG(10/8)
160 GAGGAG
BsmAI 310 GAGAC GTCTC(1/5)
451 GAGAC
Bsp1286I 359 GTGCTC G[AGT]GC[ACT]^C
821 GGGCCC
DdeI 971 CTCAG C^T[ACGT]AG
1018 CTCAG
DsaI 716 CCATGG C^C[GA][CT]GG
722 CCATGG
EcoT14I 716 CCATGG C^C[AT][AT]GG
722 CCATGG
HinfI 477 GAATC G^A[ACGT]TC
744 GATTC
MaeI 625 CTAG C^TAG
748 CTAG
MamI 394 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
984 GATCGCCATC
MboII 498 GAAGA GAAGA(8/7)
649 GAAGA
NcoI 716 CCATGG C^CATGG
722 CCATGG
PpuMI 695 AGGACCC [GA]G^G[AT]CC[CT]
911 AGGACCC
PvuII 505 CAGCTG CAG^CTG
932 CAGCTG
RsaI 126 GTAC GT^AC
410 GTAC
SfaNI 660 GCATC GCATC(5/9)
964 GCATC
StyI 716 CCATGG C^C[AT][AT]GG
722 CCATGG
TfiI 477 GAATC G^A[AT]TC
744 GATTC
TseI 110 GCAGC G^C[AT]GC
853 GCAGC
XbaI 624 TCTAGA T^CTAGA
747 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI BalI BcnI
BpmI BsaJI BsgI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
BstYI CfoI Cfr13I DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I ScrFI
TspRI XcmI XhoII