Restriction map for
pFN21AE1526
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KasI MaeI MfeI MflI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 464 GGTACC G^GTACC
AlwI 1080 GGATC GGATC(4/5)
ApaI 374 GGGCCC GGGCC^C
Asp718I 464 GGTACC G^GTACC
AvaI 114 CTCGGG C^[CT]CG[GA]G
BalI 161 TGGCCA TGG^CCA
BsaBI 159 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 350 CGGCCG CG[GA][CT]^CG
BsmI 760 GCATTC GAATGC(1/-1)
BsoBI 114 CTCGGG C^[CT]CG[GA]G
BsrFI 461 GCCGGT [GA]^CCGG[CT]
BsrI 951 ACTGG ACTGG(1/-1)
BssSI 874 CACGAG CACGAG(-5/-1)
Cfr10I 461 GCCGGT [GA]^CCGG[CT]
DpnII 1081 GATC ^GATC
EagI 350 CGGCCG C^GGCCG
EclXI 350 CGGCCG C^GGCCG
Eco52I 350 CGGCCG C^GGCCG
HaeII 39 GGCGCT [GA]GCGC^[CT]
KpnI 464 GGTACC GGTAC^C
KspI 170 CCGCGG CCGC^GG
MamI 159 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1081 GATC ^GATC
MluNI 161 TGGCCA TGG^CCA
MscI 161 TGGCCA TGG^CCA
MslI 819 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 818 CCATGG C^CATGG
NdeII 1081 GATC ^GATC
NspI 443 GCATGC [GA]CATG^[CT]
PflMI 831 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 805 GGGACCC [GA]G^G[AT]CC[CT]
SacI 964 GAGCTC GAGCT^C
SacII 170 CCGCGG CCGC^GG
Sau3AI 1081 GATC ^GATC
SphI 443 GCATGC GCATG^C
Tsp509I 121 AATT ^AATT
Van91I 831 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 641 CCATCTCCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 221 GGCGTC G[GA]^CG[CT]C
604 GACGCC
AfaI 465 GTAC GT^AC
1008 GTAC
BanI 464 GGTACC G^G[CT][GA]CC
568 GGTGCC
BbrPI 519 CACGTG CAC^GTG
880 CACGTG
BcnI 48 CCCGG CC^[GC]GG
125 CCCGG
BsaAI 519 CACGTG [CT]AC^GT[GA]
880 CACGTG
BsaHI 221 GGCGTC G[GA]^CG[CT]C
604 GACGCC
BsmFI 486 GGGAC GGGAC(10/14)
805 GGGAC
BstF5I 543 CATCC GGATG(2/0)
824 GGATG
BstXI 432 CCAGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
818 CCATGGGGATGG
DsaI 170 CCGCGG C^C[GA][CT]GG
818 CCATGG
EcoT14I 144 CCAAGG C^C[AT][AT]GG
818 CCATGG
FokI 543 CATCC GGATG(9/13)
824 GGATG
Hin1I 221 GGCGTC G[GA]^CG[CT]C
604 GACGCC
HphI 906 GGTGA GGTGA(8/7)
167 TCACC
MaeIII 309 GTCAC ^GT[ACGT]AC
907 GTGAC
NciI 48 CCCGG CC^[GC]GG
125 CCCGG
PmaCI 519 CACGTG CAC^GTG
880 CACGTG
PmlI 519 CACGTG CAC^GTG
880 CACGTG
RsaI 465 GTAC GT^AC
1008 GTAC
SmlI 15 CTCAAG C^T[CT][GA]AG
745 CTCAAG
StyI 144 CCAAGG C^C[AT][AT]GG
818 CCATGG
Tsp45I 309 GTCAC ^GT[GC]AC
907 GTGAC
TspRI 226 CAGTG CA[GC]TG[ACGT][ACGT]^
327 CACTG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BanII BbvI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI Bsp1286I BspMI BssKI
BstAPI BstNI BstUI Cac8I CfoI
Cfr13I DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
Ksp632I MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NlaIII
NlaIV PstI PvuII SapI Sau96I
ScrFI SfcI TseI