Restriction map for
pFN21AE3646
##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeI MaeII
MaeIII MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AfaI 222 GTAC GT^AC
AseI 460 ATTAAT AT^TAAT
AsnI 460 ATTAAT AT^TAAT
Asp700I 196 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 34 CTCGGG C^[CT]CG[GA]G
BalI 341 TGGCCA TGG^CCA
BanI 424 GGTGCC G^G[CT][GA]CC
BlpI 233 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 233 GCTCAGC GC^T[ACGT]AGC
BsaJI 506 CCTTGG C^C[ACGT][ACGT]GG
BsmAI 439 GAGAC GTCTC(1/5)
BsoBI 34 CTCGGG C^[CT]CG[GA]G
BsrFI 575 GCCGGT [GA]^CCGG[CT]
BsrI 43 ACTGG ACTGG(1/-1)
BssKI 81 CCAGG ^CC[ACGT]GG
BstAPI 448 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 396 GGATG GGATG(2/0)
BstNI 81 CCAGG CC^[AT]GG
BstXI 516 CCATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 497 AGATCC [GA]^GATC[CT]
CelII 233 GCTCAGC GC^T[ACGT]AGC
Cfr10I 575 GCCGGT [GA]^CCGG[CT]
EaeI 341 TGGCCA [CT]^GGCC[GA]
EarI 469 GAAGAG CTCTTC(1/4)
EcoRII 81 CCAGG ^CC[AT]GG
EcoT14I 506 CCTTGG C^C[AT][AT]GG
FokI 396 GGATG GGATG(9/13)
HaeIII 342 GGCC GG^CC
HapII 576 CCGG C^CGG
HpaII 576 CCGG C^CGG
Ksp632I 469 GAAGAG CTCTTC(1/4)
MflI 497 AGATCC [GA]^GATC[CT]
MluNI 341 TGGCCA TGG^CCA
MscI 341 TGGCCA TGG^CCA
MseI 461 TTAA T^TAA
MspI 576 CCGG C^CGG
MvaI 81 CCAGG CC^[AT]GG
PshBI 460 ATTAAT AT^TAAT
PstI 239 CTGCAG CTGCA^G
RsaI 222 GTAC GT^AC
SapI 469 GAAGAGC GCTCTTC(1/4)
ScrFI 81 CCAGG CC^[ACGT]GG
SfcI 239 CTGCAG C^T[GA][CT]AG
SgrAI 574 CGCCGGTG C[GA]^CCGG[CT]G
StyI 506 CCTTGG C^C[AT][AT]GG
Tru9I 461 TTAA T^TAA
XhoII 497 AGATCC [GA]^GATC[CT]
XmnI 196 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 492 CCGC CCGC(-3/-1)
573 CCGC
AlwI 498 GATCC GGATC(4/5)
84 GGATC
AspHI 16 GAGCTC G[AT]GC[AT]^C
472 GAGCTC
AvaII 322 GGTCC G^G[AT]CC
510 GGACC
BanII 16 GAGCTC G[GA]GC[CT]^C
472 GAGCTC
BbvI 241 GCAGC GCAGC(8/12)
238 GCTGC
BmyI 16 GAGCTC G[AGT]GC[ACT]^C
472 GAGCTC
BsiHKAI 16 GAGCTC G[AT]GC[AT]^C
472 GAGCTC
BsmFI 38 GGGAC GGGAC(10/14)
179 GGGAC
Bsp1286I 16 GAGCTC G[AGT]GC[ACT]^C
472 GAGCTC
Cac8I 564 GCAAGC GC[ACGT]^[ACGT]GC
568 GCCAGC
Cfr13I 322 GGTCC G^G[ACGT]CC
510 GGACC
DpnII 85 GATC ^GATC
498 GATC
MboI 85 GATC ^GATC
498 GATC
MboII 291 GAAGA GAAGA(8/7)
469 GAAGA
MslI 333 CATTGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
517 CATGAAAATG
MspA1I 236 CAGCTG C[AC]G^C[GT]G
242 CAGCTG
NdeII 85 GATC ^GATC
498 GATC
PvuII 236 CAGCTG CAG^CTG
242 CAGCTG
SacI 16 GAGCTC GAGCT^C
472 GAGCTC
Sau3AI 85 GATC ^GATC
498 GATC
Sau96I 322 GGTCC G^G[ACGT]CC
510 GGACC
TseI 238 GCTGC G^C[AT]GC
241 GCAGC
TspRI 42 CACTG CA[GC]TG[ACGT][ACGT]^
183 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BsiYI BslI DdeI Fnu4HI
HinfI ItaI MnlI MwoI NlaIII
NlaIV SfaNI TaqI TfiI TthHB8I