Restriction map for
pFN21AE1376
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HindIII HinfI
HpaI HphI KasI KpnI MaeI
MaeIII MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 287 CGCG CG^CG
AcyI 571 GACGCC G[GA]^CG[CT]C
AlwNI 359 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 538 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 509 TGCGCA TGC^GCA
BalI 242 TGGCCA TGG^CCA
BanI 64 GGCACC G^G[CT][GA]CC
BanII 528 GGGCTC G[GA]GC[CT]^C
BbsI 544 GTCTTC GAAGAC(2/6)
BbvCI 523 GCTGAGG CCTCAGC(-5/-2)
BglI 235 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 544 GTCTTC GAAGAC(2/6)
BsaHI 571 GACGCC G[GA]^CG[CT]C
BseRI 427 GAGGAG GAGGAG(10/8)
BsmFI 119 GGGAC GGGAC(10/14)
BstF5I 231 GGATG GGATG(2/0)
BstUI 287 CGCG CG^CG
CfoI 510 GCGC GCG^C
CpoI 130 CGGTCCG CG^G[AT]CCG
DpnII 81 GATC ^GATC
DraIII 66 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 49 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 236 CCATGG C^C[AT][AT]GG
FokI 231 GGATG GGATG(9/13)
FspI 509 TGCGCA TGC^GCA
HhaI 510 GCGC GCG^C
Hin1I 571 GACGCC G[GA]^CG[CT]C
HinP1I 510 GCGC G^CGC
HincII 517 GTCAAC GT[CT]^[GA]AC
HindII 517 GTCAAC GT[CT]^[GA]AC
KspI 286 CCGCGG CCGC^GG
MboI 81 GATC ^GATC
MluNI 242 TGGCCA TGG^CCA
MscI 242 TGGCCA TGG^CCA
MslI 30 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 287 CGCG CG^CG
NcoI 236 CCATGG C^CATGG
NdeII 81 GATC ^GATC
NspI 221 ACATGC [GA]CATG^[CT]
PflFI 538 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 119 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 193 CAGCTG CAG^CTG
RsrII 130 CGGTCCG CG^G[AT]CCG
SacII 286 CCGCGG CCGC^GG
Sau3AI 81 GATC ^GATC
SfaNI 232 GATGC GCATC(5/9)
StyI 236 CCATGG C^C[AT][AT]GG
Tth111I 538 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 404 CCAGCGGGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 120 GGACC G^G[AT]CC
131 GGTCC
BcnI 116 CCGGG CC^[GC]GG
228 CCGGG
BmyI 63 GGGCAC G[AGT]GC[ACT]^C
528 GGGCTC
BpmI 205 CTGGAG CTGGAG(16/14)
448 CTGGAG
BsiYI 49 CCTGGCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
285 CCCGCGGGAGG
BslI 49 CCTGGCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
285 CCCGCGGGAGG
Bsp1286I 63 GGGCAC G[AGT]GC[ACT]^C
528 GGGCTC
BsrI 204 ACTGG ACTGG(1/-1)
69 CCAGT
Cac8I 358 GCAGGC GC[ACGT]^[ACGT]GC
407 GCGGGC
DraII 119 GGGACCT [GA]G^G[ACGT]CC[CT]
360 AGGCCCT
EaeI 152 CGGCCA [CT]^GGCC[GA]
242 TGGCCA
EarI 4 GAAGAG CTCTTC(1/4)
307 GAAGAG
EcoO109I 119 GGGACCT [GA]G^G[ACGT]CC[CT]
360 AGGCCCT
HapII 116 CCGG C^CGG
228 CCGG
HgaI 355 GACGC GACGC(5/10)
571 GACGC
HpaII 116 CCGG C^CGG
228 CCGG
Ksp632I 4 GAAGAG CTCTTC(1/4)
307 GAAGAG
MaeII 393 ACGT A^CGT
498 ACGT
MspI 116 CCGG C^CGG
228 CCGG
NciI 116 CCGGG CC^[GC]GG
228 CCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BbvI BsaI BsaJI
BsgI BsmAI BssKI BstNI Cfr13I
DdeI DsaI EcoRII FauI Fnu4HI
HaeIII ItaI MboII MnlI MspA1I
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TseI TspRI