Restriction map for
pFN21AE6333
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# NO CUT ENZYMES ( 131 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MroI MseI MslI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 109 GACGTC GACGT^C
AflIII 243 ACACGT A^C[GA][CT]GT
AlwI 120 GATCC GGATC(4/5)
AviII 425 TGCGCA TGC^GCA
BanI 829 GGCGCC G^G[CT][GA]CC
BanII 451 GAGCCC G[GA]GC[CT]^C
BbeI 829 GGCGCC GGCGC^C
BbsI 925 GTCTTC GAAGAC(2/6)
BclI 95 TGATCA T^GATCA
BfaI 197 CTAG C^TAG
BglI 385 GCCTGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 187 CTCCAG CTGGAG(16/14)
BpuAI 925 GTCTTC GAAGAC(2/6)
BsaBI 546 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 214 GAGGAG GAGGAG(10/8)
BsmAI 150 GTCTC GTCTC(1/5)
Bsp1407I 605 TGTACA T^GTACA
BsrGI 605 TGTACA T^GTACA
BstAPI 201 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 119 AGATCC [GA]^GATC[CT]
EarI 715 CTCTTC CTCTTC(1/4)
FauI 431 CCCGC CCCGC(4/6)
FbaI 95 TGATCA T^GATCA
FspI 425 TGCGCA TGC^GCA
HgaI 340 GACGC GACGC(5/10)
HphI 448 GGTGA GGTGA(8/7)
KasI 829 GGCGCC G^GCGCC
Ksp632I 715 CTCTTC CTCTTC(1/4)
MaeI 197 CTAG C^TAG
MamI 546 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 119 AGATCC [GA]^GATC[CT]
MspA1I 706 CCGCTG C[AC]G^C[GT]G
NarI 829 GGCGCC GG^CGCC
NcoI 177 CCATGG C^CATGG
NspI 750 GCATGC [GA]CATG^[CT]
PflMI 477 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 182 GCATC GCATC(5/9)
SfoI 829 GGCGCC GGC^GCC
SmaI 344 CCCGGG CCC^GGG
SphI 750 GCATGC GCATG^C
SspBI 605 TGTACA T^GTACA
StuI 191 AGGCCT AGG^CCT
TaqI 107 TCGA T^CGA
Tsp45I 158 GTGAC ^GT[GC]AC
TthHB8I 107 TCGA T^CGA
Van91I 477 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 41 CCAAGTTCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 119 AGATCC [GA]^GATC[CT]
XmaCI 344 CCCGGG C^CCGGG
XmaI 344 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 349 GAATTC [GA]^AATT[CT]
910 GAATTC
Alw44I 358 GTGCAC G^TGCAC
406 GTGCAC
AlwNI 56 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
202 CAGATTCTG
ApaLI 358 GTGCAC G^TGCAC
406 GTGCAC
ApoI 349 GAATTC [GA]^AATT[CT]
910 GAATTC
AspHI 358 GTGCAC G[AT]GC[AT]^C
406 GTGCAC
AvaI 86 CTCGGG C^[CT]CG[GA]G
344 CCCGGG
BalI 392 TGGCCA TGG^CCA
768 TGGCCA
BsiHKAI 358 GTGCAC G[AT]GC[AT]^C
406 GTGCAC
BsoBI 86 CTCGGG C^[CT]CG[GA]G
344 CCCGGG
BspMI 656 ACCTGC ACCTGC(4/8)
810 GCAGGT
BstF5I 183 CATCC GGATG(2/0)
417 GGATG
DraIII 409 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
478 CACGCCGTG
EaeI 392 TGGCCA [CT]^GGCC[GA]
768 TGGCCA
EcoRI 349 GAATTC G^AATTC
910 GAATTC
EcoT14I 177 CCATGG C^C[AT][AT]GG
455 CCAAGG
FokI 183 CATCC GGATG(9/13)
417 GGATG
HapII 345 CCGG C^CGG
566 CCGG
HinfI 204 GATTC G^A[ACGT]TC
258 GATTC
HpaII 345 CCGG C^CGG
566 CCGG
MaeII 110 ACGT A^CGT
245 ACGT
MaeIII 82 GTTAC ^GT[ACGT]AC
158 GTGAC
MluNI 392 TGGCCA TGG^CCA
768 TGGCCA
MscI 392 TGGCCA TGG^CCA
768 TGGCCA
MspI 345 CCGG C^CGG
566 CCGG
PstI 208 CTGCAG CTGCA^G
571 CTGCAG
StyI 177 CCATGG C^C[AT][AT]GG
455 CCAAGG
TfiI 204 GATTC G^A[AT]TC
258 GATTC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
Fnu4HI HaeII HaeIII HhaI Hin1I
HinP1I ItaI MboI MboII MnlI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI TseI Tsp509I TspRI