Restriction map for
pFN21AE4440
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbrPI BbsI
BcgI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MaeI MaeII MamI
MfeI MluI MseI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 794 TCCGGA T^CCGGA
AcsI 219 GAATTT [GA]^AATT[CT]
AcyI 256 GGCGCC G[GA]^CG[CT]C
ApoI 219 GAATTT [GA]^AATT[CT]
BalI 1043 TGGCCA TGG^CCA
BamHI 194 GGATCC G^GATCC
BanI 256 GGCGCC G^G[CT][GA]CC
BbeI 256 GGCGCC GGCGC^C
BciVI 84 GGATAC GTATCC(6/5)
BsaHI 256 GGCGCC G[GA]^CG[CT]C
BsaWI 794 TCCGGA [AT]^CCGG[AT]
BseAI 794 TCCGGA T^CCGGA
BsgI 653 GTGCAG GTGCAG(16/14)
BsiEI 811 CGGTCG CG[GA][CT]^CG
BspEI 794 TCCGGA T^CCGGA
EcoNI 615 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 102 ATGCAT ATGCA^T
HaeII 256 GGCGCC [GA]GCGC^[CT]
Hin1I 256 GGCGCC G[GA]^CG[CT]C
KasI 256 GGCGCC G^GCGCC
MaeIII 46 GTCAC ^GT[ACGT]AC
MluNI 1043 TGGCCA TGG^CCA
MroI 794 TCCGGA T^CCGGA
MscI 1043 TGGCCA TGG^CCA
MslI 750 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 256 GGCGCC GG^CGCC
NsiI 102 ATGCAT ATGCA^T
PpuMI 866 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1351 CTGCAG CTGCA^G
SacI 232 GAGCTC GAGCT^C
SfaNI 104 GCATC GCATC(5/9)
SfoI 256 GGCGCC GGC^GCC
StuI 942 AGGCCT AGG^CCT
TfiI 403 GATTC G^A[AT]TC
Tsp45I 46 GTCAC ^GT[GC]AC
Tsp509I 220 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 622 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1294 CAGGAGCTG
ApaI 1130 GGGCCC GGGCC^C
1152 GGGCCC
AspHI 232 GAGCTC G[AT]GC[AT]^C
892 GAGCAC
BglI 660 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1315 GCCATTGAGGC
BsiHKAI 232 GAGCTC G[AT]GC[AT]^C
892 GAGCAC
BsmAI 172 GAGAC GTCTC(1/5)
210 GAGAC
BsrBI 963 GAGCGG CCGCTC(-3/-3)
988 GAGCGG
BstYI 194 GGATCC [GA]^GATC[CT]
791 AGATCC
DpnII 195 GATC ^GATC
792 GATC
EaeI 1043 TGGCCA [CT]^GGCC[GA]
1313 CGGCCA
HphI 93 GGTGA GGTGA(8/7)
427 GGTGA
MboI 195 GATC ^GATC
792 GATC
MflI 194 GGATCC [GA]^GATC[CT]
791 AGATCC
NdeII 195 GATC ^GATC
792 GATC
Sau3AI 195 GATC ^GATC
792 GATC
SfcI 752 CTGTAG C^T[GA][CT]AG
1351 CTGCAG
SmaI 1133 CCCGGG CCC^GGG
1193 CCCGGG
SmlI 322 CTTGAG C^T[CT][GA]AG
1207 CTTGAG
XcmI 377 CCAAGGATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
768 CCACCCGAGCCTTGG
XhoII 194 GGATCC [GA]^GATC[CT]
791 AGATCC
XmaCI 1133 CCCGGG C^CCGGG
1193 CCCGGG
XmaI 1133 CCCGGG C^CCGGG
1193 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanII BbvCI BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PflMI PleI PvuII SapI Sau96I
ScrFI StyI TseI TspRI Van91I