Restriction map for
pFN21AE4580
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
BbeI BbrPI BciVI BclI BfrI
BglII BseAI BsiWI BsmI BspEI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CpoI
DraIII DrdI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
StuI SwaI Tsp509I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 1517 GACGCC G[GA]^CG[CT]C
AocI 1177 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1766 AGCGCT AGC^GCT
ApaI 1147 GGGCCC GGGCC^C
AspI 1322 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 567 CCTAGG C^CTAGG
BalI 2036 TGGCCA TGG^CCA
BamHI 1629 GGATCC G^GATCC
BlnI 567 CCTAGG C^CTAGG
BsaAI 2134 TACGTG [CT]AC^GT[GA]
BsaHI 1517 GACGCC G[GA]^CG[CT]C
BsaI 1954 GAGACC GGTCTC(1/5)
BsaWI 119 ACCGGA [AT]^CCGG[AT]
BsgI 1963 GTGCAG GTGCAG(16/14)
BsmBI 2143 GAGACG CGTCTC(1/5)
Bsp1407I 743 TGTACA T^GTACA
BspDI 2041 ATCGAT AT^CGAT
BsrGI 743 TGTACA T^GTACA
BsrI 669 ACTGG ACTGG(1/-1)
BstEII 1188 GGTGACC G^GT[ACGT]ACC
BstPI 1188 GGTGACC G^GT[ACGT]ACC
Bsu36I 1177 CCTGAGG CC^T[ACGT]AGG
ClaI 2041 ATCGAT AT^CGAT
DraI 1195 TTTAAA TTT^AAA
Eco47III 1766 AGCGCT AGC^GCT
Eco81I 1177 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1188 GGTGACC G^GT[ACGT]ACC
HgaI 1517 GACGC GACGC(5/10)
Hin1I 1517 GACGCC G[GA]^CG[CT]C
KspI 88 CCGCGG CCGC^GG
MluNI 2036 TGGCCA TGG^CCA
MscI 2036 TGGCCA TGG^CCA
NcoI 2199 CCATGG C^CATGG
NruI 1722 TCGCGA TCG^CGA
NspI 780 GCATGC [GA]CATG^[CT]
PflFI 1322 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 543 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1895 CGATCG CGAT^CG
SacII 88 CCGCGG CCGC^GG
SapI 1015 GAAGAGC GCTCTTC(1/4)
SbfI 759 CCTGCAGG CCTGCA^GG
SexAI 878 ACCTGGT A^CC[AT]GGT
SmlI 826 CTCAAG C^T[CT][GA]AG
SphI 780 GCATGC GCATG^C
Sse8387I 759 CCTGCAGG CCTGCA^GG
SspBI 743 TGTACA T^GTACA
Tth111I 1322 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 543 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbsI 2223 GAAGAC GAAGAC(2/6)
2337 GAAGAC
BcgI 306 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
981 GCAAAAACCTCG
BfaI 329 CTAG C^TAG
568 CTAG
BglI 84 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1604 GCCAGCGGGGC
BlpI 1769 GCTCAGC GC^T[ACGT]AGC
2355 GCTCAGC
Bpu1102I 1769 GCTCAGC GC^T[ACGT]AGC
2355 GCTCAGC
BpuAI 2223 GAAGAC GAAGAC(2/6)
2337 GAAGAC
BsaBI 1624 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2034 GATGGCCATC
CelII 1769 GCTCAGC GC^T[ACGT]AGC
2355 GCTCAGC
EagI 38 CGGCCG C^GGCCG
56 CGGCCG
EarI 654 GAAGAG CTCTTC(1/4)
1015 GAAGAG
EclXI 38 CGGCCG C^GGCCG
56 CGGCCG
Eco52I 38 CGGCCG C^GGCCG
56 CGGCCG
Ksp632I 654 GAAGAG CTCTTC(1/4)
1015 GAAGAG
MaeI 329 CTAG C^TAG
568 CTAG
MamI 1624 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2034 GATGGCCATC
PleI 1287 GACTC GAGTC(4/5)
1597 GAGTC
SmaI 752 CCCGGG CCC^GGG
1976 CCCGGG
Tsp45I 451 GTGAC ^GT[GC]AC
1189 GTGAC
XmaCI 752 CCCGGG C^CCGGG
1976 CCCGGG
XmaI 752 CCCGGG C^CCGGG
1976 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
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AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BmyI
BpmI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI TspRI
TthHB8I XcmI XhoII