Restriction map for
pFN21AE1304
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HaeIII HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MflI MluI MluNI MroI
MscI MspA1I MspI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TspRI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AluI 552 AGCT AG^CT
AseI 475 ATTAAT AT^TAAT
AsnI 475 ATTAAT AT^TAAT
Asp700I 246 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 189 GGACC G^G[AT]CC
BanI 283 GGTGCC G^G[CT][GA]CC
BanII 747 GAGCCC G[GA]GC[CT]^C
BfaI 421 CTAG C^TAG
BpmI 43 CTGGAG CTGGAG(16/14)
BseRI 19 GAGGAG GAGGAG(10/8)
BsgI 819 CTGCAC GTGCAG(16/14)
BsiYI 256 CCGTATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 256 CCGTATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrDI 468 CATTGC GCAATG(2/0)
BssHII 24 GCGCGC G^CGCGC
BstXI 473 CCATTAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 189 GGACC G^G[ACGT]CC
DraII 188 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 803 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 457 GACTGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 188 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 511 GATATC GAT^ATC
MaeI 421 CTAG C^TAG
MaeII 836 ACGT A^CGT
MfeI 356 CAATTG C^AATTG
MslI 474 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 356 CAATTG C^AATTG
PleI 53 GAGTC GAGTC(4/5)
PpuMI 188 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 475 ATTAAT AT^TAAT
Sau96I 189 GGACC G^G[ACGT]CC
XcmI 776 CCAGAGCCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 246 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 25 CGCG CG^CG
30 CGCG
AciI 29 CCGC CCGC(-3/-1)
5 GCGG
AcsI 246 GAATTT [GA]^AATT[CT]
625 GAATTT
AlwI 487 GATCC GGATC(4/5)
763 GATCC
ApoI 246 GAATTT [GA]^AATT[CT]
625 GAATTT
BbsI 304 GAAGAC GAAGAC(2/6)
55 GTCTTC
BbvI 496 GCAGC GCAGC(8/12)
772 GCTGC
BmyI 313 GGGCAC G[AGT]GC[ACT]^C
747 GAGCCC
BpuAI 304 GAAGAC GAAGAC(2/6)
55 GTCTTC
Bsp1286I 313 GGGCAC G[AGT]GC[ACT]^C
747 GAGCCC
BspHI 96 TCATGA T^CATGA
669 TCATGA
BstUI 25 CGCG CG^CG
30 CGCG
Cac8I 24 GCGCGC GC[ACGT]^[ACGT]GC
817 GCCTGC
DpnII 487 GATC ^GATC
763 GATC
DraI 195 TTTAAA TTT^AAA
628 TTTAAA
MaeIII 344 GTGAC ^GT[ACGT]AC
691 GTGAC
MboI 487 GATC ^GATC
763 GATC
MboII 304 GAAGA GAAGA(8/7)
56 TCTTC
MvnI 25 CGCG CG^CG
30 CGCG
NdeII 487 GATC ^GATC
763 GATC
RcaI 96 TCATGA T^CATGA
669 TCATGA
Sau3AI 487 GATC ^GATC
763 GATC
TfiI 716 GAATC G^A[AT]TC
811 GAATC
TseI 496 GCAGC G^C[AT]GC
772 GCTGC
Tsp45I 344 GTGAC ^GT[GC]AC
691 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI BsaJI BsrI BssKI BstF5I
BstNI CfoI DdeI EcoRII Fnu4HI
FokI HhaI HinP1I HinfI HphI
ItaI MnlI MseI MvaI MwoI
NlaIII NlaIV RsaI ScrFI SfaNI
Tru9I Tsp509I