Restriction map for
pFN21AE4418
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AviII
AvrII BamHI BanII BbeI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BspEI BspLU11I BsrDI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT14I
FseI FspI HaeII HincII HindII
HindIII HpaI HphI KasI KspI
MamI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SpeI SphI Sse8387I
StuI StyI SwaI Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 1271 GGTACC G^GTACC
AcyI 1052 GACGCC G[GA]^CG[CT]C
AhdI 1122 GACGATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 558 GATCC GGATC(4/5)
Asp718I 1271 GGTACC G^GTACC
AspEI 1122 GACGATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 270 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 422 TGGCCA TGG^CCA
BanI 1271 GGTACC G^G[CT][GA]CC
BbrPI 528 CACGTG CAC^GTG
BclI 50 TGATCA T^GATCA
BfaI 815 CTAG C^TAG
BpmI 683 CTGGAG CTGGAG(16/14)
BsaHI 1052 GACGCC G[GA]^CG[CT]C
BsaI 460 GAGACC GGTCTC(1/5)
BsgI 1242 GTGCAG GTGCAG(16/14)
Bsp1407I 2 TGTACA T^GTACA
BspDI 881 ATCGAT AT^CGAT
BspMI 1169 ACCTGC ACCTGC(4/8)
BsrBI 1159 GAGCGG CCGCTC(-3/-3)
BsrGI 2 TGTACA T^GTACA
BstAPI 144 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 497 TTCGAA TT^CGAA
BstYI 557 AGATCC [GA]^GATC[CT]
ClaI 881 ATCGAT AT^CGAT
DraIII 487 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 422 TGGCCA [CT]^GGCC[GA]
Eam1105I 1122 GACGATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 719 CTCTTC CTCTTC(1/4)
EcoNI 229 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 733 GATATC GAT^ATC
EcoT22I 885 ATGCAT ATGCA^T
FauI 1039 GCGGG CCCGC(4/6)
FbaI 50 TGATCA T^GATCA
HgaI 1052 GACGC GACGC(5/10)
Hin1I 1052 GACGCC G[GA]^CG[CT]C
KpnI 1271 GGTACC GGTAC^C
Ksp632I 719 CTCTTC CTCTTC(1/4)
MaeI 815 CTAG C^TAG
MboII 720 TCTTC GAAGA(8/7)
MfeI 203 CAATTG C^AATTG
MflI 557 AGATCC [GA]^GATC[CT]
MluNI 422 TGGCCA TGG^CCA
MscI 422 TGGCCA TGG^CCA
MspA1I 61 CAGCTG C[AC]G^C[GT]G
MunI 203 CAATTG C^AATTG
NlaIV 1271 GGTACC GG[ACGT]^[ACGT]CC
NsiI 885 ATGCAT ATGCA^T
NspI 301 ACATGC [GA]CATG^[CT]
NspV 497 TTCGAA TT^CGAA
PaeR7I 1146 CTCGAG C^TCGAG
PflFI 270 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 528 CACGTG CAC^GTG
PmlI 528 CACGTG CAC^GTG
PvuII 61 CAGCTG CAG^CTG
SfuI 497 TTCGAA TT^CGAA
SmaI 1228 CCCGGG CCC^GGG
SnaBI 829 TACGTA TAC^GTA
SspBI 2 TGTACA T^GTACA
SspI 1192 AATATT AAT^ATT
TfiI 495 GATTC G^A[AT]TC
Tth111I 270 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1146 CTCGAG C^TCGAG
XhoII 557 AGATCC [GA]^GATC[CT]
XmaCI 1228 CCCGGG C^CCGGG
XmaI 1228 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 1038 CGCG CG^CG
1262 CGCG
AcsI 250 GAATTT [GA]^AATT[CT]
948 AAATTC
ApoI 250 GAATTT [GA]^AATT[CT]
948 AAATTC
AvaI 1146 CTCGAG C^[CT]CG[GA]G
1228 CCCGGG
AvaII 27 GGACC G^G[AT]CC
325 GGACC
BcnI 1228 CCCGG CC^[GC]GG
1229 CCGGG
BsaAI 528 CACGTG [CT]AC^GT[GA]
829 TACGTA
BsaWI 1064 ACCGGA [AT]^CCGG[AT]
1274 ACCGGA
BsoBI 1146 CTCGAG C^[CT]CG[GA]G
1228 CCCGGG
BspHI 449 TCATGA T^CATGA
1235 TCATGA
BstUI 1038 CGCG CG^CG
1262 CGCG
Cac8I 32 GCCAGC GC[ACGT]^[ACGT]GC
59 GCCAGC
CfoI 1037 GCGC GCG^C
1261 GCGC
Cfr13I 27 GGACC G^G[ACGT]CC
325 GGACC
HaeIII 97 GGCC GG^CC
423 GGCC
HhaI 1037 GCGC GCG^C
1261 GCGC
HinP1I 1037 GCGC G^CGC
1261 GCGC
MvnI 1038 CGCG CG^CG
1262 CGCG
NciI 1228 CCCGG CC^[GC]GG
1229 CCGGG
PleI 979 GACTC GAGTC(4/5)
1144 GACTC
RcaI 449 TCATGA T^CATGA
1235 TCATGA
Sau96I 27 GGACC G^G[ACGT]CC
325 GGACC
SfcI 345 CTATAG C^T[GA][CT]AG
983 CTGTAG
Tsp45I 310 GTCAC ^GT[GC]AC
526 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwNI
ApaLI AspHI BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI DpnII EcoRII Fnu4HI FokI
HapII HinfI HpaII ItaI MaeII
MaeIII MboI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI SmlI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I