################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII AccIII AclI AcyI AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI AscI AseI AsnI AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HpaII KasI KspI MaeI MamI MboI MflI MluI MluNI MroI MscI MspI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 964 GGTACC G^GTACC AccI 1009 GTCTAC GT^[AC][GT]AC AflII 216 CTTAAG C^TTAAG Alw44I 64 GTGCAC G^TGCAC ApaI 103 GGGCCC GGGCC^C ApaLI 64 GTGCAC G^TGCAC Asp700I 33 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 964 GGTACC G^GTACC AspHI 64 GTGCAC G[AT]GC[AT]^C BanI 964 GGTACC G^G[CT][GA]CC BfrI 216 CTTAAG C^TTAAG BpmI 815 CTGGAG CTGGAG(16/14) BsaI 73 GGTCTC GGTCTC(1/5) BsgI 974 CTGCAC GTGCAG(16/14) BsiHKAI 64 GTGCAC G[AT]GC[AT]^C BsmI 640 GCATTC GAATGC(1/-1) Bsp1407I 731 TGTACA T^GTACA BspHI 956 TCATGA T^CATGA BsrDI 528 CATTGC GCAATG(2/0) BsrGI 731 TGTACA T^GTACA BsrI 506 CCAGT ACTGG(1/-1) BstAPI 57 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 560 TTCGAA TT^CGAA DraIII 194 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 154 GAAGAG CTCTTC(1/4) EcoT14I 378 CCTTGG C^C[AT][AT]GG HincII 1074 GTTAAC GT[CT]^[GA]AC HindII 1074 GTTAAC GT[CT]^[GA]AC HindIII 1066 AAGCTT A^AGCTT HpaI 1074 GTTAAC GTT^AAC HphI 840 TCACC GGTGA(8/7) KpnI 964 GGTACC GGTAC^C Ksp632I 154 GAAGAG CTCTTC(1/4) MaeII 714 ACGT A^CGT MfeI 830 CAATTG C^AATTG MunI 830 CAATTG C^AATTG NspV 560 TTCGAA TT^CGAA PpuMI 80 GGGACCT [GA]G^G[AT]CC[CT] PstI 55 CTGCAG CTGCA^G RcaI 956 TCATGA T^CATGA SfuI 560 TTCGAA TT^CGAA SspBI 731 TGTACA T^GTACA StuI 630 AGGCCT AGG^CCT StyI 378 CCTTGG C^C[AT][AT]GG TaqI 561 TCGA T^CGA TthHB8I 561 TCGA T^CGA XmnI 33 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 26 CGCG CG^CG 180 CGCG AvaI 76 CTCGGG C^[CT]CG[GA]G 1039 CCCGAG BanII 103 GGGCCC G[GA]GC[CT]^C 365 GGGCTC BsiYI 21 CCTGGCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 270 CCGTATGCAGG BslI 21 CCTGGCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 270 CCGTATGCAGG BsoBI 76 CTCGGG C^[CT]CG[GA]G 1039 CCCGAG BspMI 276 GCAGGT ACCTGC(4/8) 802 GCAGGT BstUI 26 CGCG CG^CG 180 CGCG DraI 427 TTTAAA TTT^AAA 919 TTTAAA DraII 80 GGGACCT [GA]G^G[ACGT]CC[CT] 102 AGGGCCC EcoO109I 80 GGGACCT [GA]G^G[ACGT]CC[CT] 102 AGGGCCC EcoRI 514 GAATTC G^AATTC 563 GAATTC MspA1I 58 CAGCTG C[AC]G^C[GT]G 781 CAGCTG MvnI 26 CGCG CG^CG 180 CGCG PvuII 58 CAGCTG CAG^CTG 781 CAGCTG SfaNI 601 GATGC GCATC(5/9) 1044 GATGC SfcI 55 CTGCAG C^T[GA][CT]AG 979 CTACAG SmlI 216 CTTAAG C^T[CT][GA]AG 904 CTCAAG TfiI 314 GATTC G^A[AT]TC 850 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvI BmyI BsaJI BseRI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DsaI EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeIII MboII MnlI MseI MslI MvaI MwoI NlaIII NlaIV PleI RsaI Sau96I ScrFI Tru9I TseI Tsp509I TspRI