Restriction map for
pFN21AE1506
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HapII HgaI
Hin1I HpaII KasI KspI MaeI
MamI MboI MflI MluI MluNI
MroI MscI MspI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 964 GGTACC G^GTACC
AccI 1009 GTCTAC GT^[AC][GT]AC
AflII 216 CTTAAG C^TTAAG
Alw44I 64 GTGCAC G^TGCAC
ApaI 103 GGGCCC GGGCC^C
ApaLI 64 GTGCAC G^TGCAC
Asp700I 33 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 964 GGTACC G^GTACC
AspHI 64 GTGCAC G[AT]GC[AT]^C
BanI 964 GGTACC G^G[CT][GA]CC
BfrI 216 CTTAAG C^TTAAG
BpmI 815 CTGGAG CTGGAG(16/14)
BsaI 73 GGTCTC GGTCTC(1/5)
BsgI 974 CTGCAC GTGCAG(16/14)
BsiHKAI 64 GTGCAC G[AT]GC[AT]^C
BsmI 640 GCATTC GAATGC(1/-1)
Bsp1407I 731 TGTACA T^GTACA
BspHI 956 TCATGA T^CATGA
BsrDI 528 CATTGC GCAATG(2/0)
BsrGI 731 TGTACA T^GTACA
BsrI 506 CCAGT ACTGG(1/-1)
BstAPI 57 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 560 TTCGAA TT^CGAA
DraIII 194 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 154 GAAGAG CTCTTC(1/4)
EcoT14I 378 CCTTGG C^C[AT][AT]GG
HincII 1074 GTTAAC GT[CT]^[GA]AC
HindII 1074 GTTAAC GT[CT]^[GA]AC
HindIII 1066 AAGCTT A^AGCTT
HpaI 1074 GTTAAC GTT^AAC
HphI 840 TCACC GGTGA(8/7)
KpnI 964 GGTACC GGTAC^C
Ksp632I 154 GAAGAG CTCTTC(1/4)
MaeII 714 ACGT A^CGT
MfeI 830 CAATTG C^AATTG
MunI 830 CAATTG C^AATTG
NspV 560 TTCGAA TT^CGAA
PpuMI 80 GGGACCT [GA]G^G[AT]CC[CT]
PstI 55 CTGCAG CTGCA^G
RcaI 956 TCATGA T^CATGA
SfuI 560 TTCGAA TT^CGAA
SspBI 731 TGTACA T^GTACA
StuI 630 AGGCCT AGG^CCT
StyI 378 CCTTGG C^C[AT][AT]GG
TaqI 561 TCGA T^CGA
TthHB8I 561 TCGA T^CGA
XmnI 33 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 26 CGCG CG^CG
180 CGCG
AvaI 76 CTCGGG C^[CT]CG[GA]G
1039 CCCGAG
BanII 103 GGGCCC G[GA]GC[CT]^C
365 GGGCTC
BsiYI 21 CCTGGCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
270 CCGTATGCAGG
BslI 21 CCTGGCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
270 CCGTATGCAGG
BsoBI 76 CTCGGG C^[CT]CG[GA]G
1039 CCCGAG
BspMI 276 GCAGGT ACCTGC(4/8)
802 GCAGGT
BstUI 26 CGCG CG^CG
180 CGCG
DraI 427 TTTAAA TTT^AAA
919 TTTAAA
DraII 80 GGGACCT [GA]G^G[ACGT]CC[CT]
102 AGGGCCC
EcoO109I 80 GGGACCT [GA]G^G[ACGT]CC[CT]
102 AGGGCCC
EcoRI 514 GAATTC G^AATTC
563 GAATTC
MspA1I 58 CAGCTG C[AC]G^C[GT]G
781 CAGCTG
MvnI 26 CGCG CG^CG
180 CGCG
PvuII 58 CAGCTG CAG^CTG
781 CAGCTG
SfaNI 601 GATGC GCATC(5/9)
1044 GATGC
SfcI 55 CTGCAG C^T[GA][CT]AG
979 CTACAG
SmlI 216 CTTAAG C^T[CT][GA]AG
904 CTCAAG
TfiI 314 GATTC G^A[AT]TC
850 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BmyI BsaJI BseRI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DsaI EcoRII FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
MaeIII MboII MnlI MseI MslI
MvaI MwoI NlaIII NlaIV PleI
RsaI Sau96I ScrFI Tru9I TseI
Tsp509I TspRI