Restriction map for
pFN21AB8900
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HinfI
HpaI KpnI MaeII MamI MfeI
MroI MseI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcyI 382 GGCGCC G[GA]^CG[CT]C
AflIII 161 ACGCGT A^C[GA][CT]GT
Asp700I 159 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 589 CCTAGG C^CTAGG
BalI 422 TGGCCA TGG^CCA
BbeI 382 GGCGCC GGCGC^C
BfaI 590 CTAG C^TAG
BglII 233 AGATCT A^GATCT
BlnI 589 CCTAGG C^CTAGG
BlpI 184 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 184 GCTCAGC GC^T[ACGT]AGC
BsaHI 382 GGCGCC G[GA]^CG[CT]C
BsiEI 151 CGGCCG CG[GA][CT]^CG
BsrFI 353 ACCGGC [GA]^CCGG[CT]
BsrI 549 ACTGG ACTGG(1/-1)
BssHII 251 GCGCGC G^CGCGC
BstXI 239 CCAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 233 AGATCT [GA]^GATC[CT]
CelII 184 GCTCAGC GC^T[ACGT]AGC
Cfr10I 353 ACCGGC [GA]^CCGG[CT]
DpnII 234 GATC ^GATC
DraII 81 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 151 CGGCCG C^GGCCG
EclXI 151 CGGCCG C^GGCCG
Eco52I 151 CGGCCG C^GGCCG
EcoNI 135 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 81 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 589 CCTAGG C^C[AT][AT]GG
HgaI 126 GACGC GACGC(5/10)
Hin1I 382 GGCGCC G[GA]^CG[CT]C
KasI 382 GGCGCC G^GCGCC
KspI 113 CCGCGG CCGC^GG
MaeI 590 CTAG C^TAG
MaeIII 584 GTGAC ^GT[ACGT]AC
MboI 234 GATC ^GATC
MflI 233 AGATCT [GA]^GATC[CT]
MluI 161 ACGCGT A^CGCGT
MluNI 422 TGGCCA TGG^CCA
MscI 422 TGGCCA TGG^CCA
NarI 382 GGCGCC GG^CGCC
NdeII 234 GATC ^GATC
PpuMI 81 GGGACCC [GA]G^G[AT]CC[CT]
SacII 113 CCGCGG CCGC^GG
SapI 533 GCTCTTC GCTCTTC(1/4)
Sau3AI 234 GATC ^GATC
SfaNI 219 GATGC GCATC(5/9)
SfoI 382 GGCGCC GGC^GCC
SmaI 121 CCCGGG CCC^GGG
StuI 29 AGGCCT AGG^CCT
StyI 589 CCTAGG C^C[AT][AT]GG
TaqI 505 TCGA T^CGA
Tsp45I 584 GTGAC ^GT[GC]AC
TthHB8I 505 TCGA T^CGA
XhoII 233 AGATCT [GA]^GATC[CT]
XmaCI 121 CCCGGG C^CCGGG
XmaI 121 CCCGGG C^CCGGG
XmnI 159 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaI 121 CCCGGG C^[CT]CG[GA]G
378 CTCGGG
BanI 382 GGCGCC G^G[CT][GA]CC
673 GGTGCC
BsmFI 81 GGGAC GGGAC(10/14)
124 GGGAC
BsoBI 121 CCCGGG C^[CT]CG[GA]G
378 CTCGGG
DdeI 185 CTCAG C^T[ACGT]AG
578 CTCAG
DsaI 56 CCACGG C^C[GA][CT]GG
113 CCGCGG
EaeI 151 CGGCCG [CT]^GGCC[GA]
422 TGGCCA
EarI 534 CTCTTC CTCTTC(1/4)
410 GAAGAG
HphI 144 GGTGA GGTGA(8/7)
562 TCACC
Ksp632I 534 CTCTTC CTCTTC(1/4)
410 GAAGAG
NlaIII 168 CATG CATG^
681 CATG
SacI 177 GAGCTC GAGCT^C
375 GAGCTC
TspRI 256 CAGTG CA[GC]TG[ACGT][ACGT]^
541 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrBI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV RsaI
Sau96I ScrFI TseI