Restriction map for
pFN21AE1474
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BglII BlnI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HindIII HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI StyI SwaI TaqI
TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1195 GACGTC GACGT^C
Acc65I 1216 GGTACC G^GTACC
AccI 1336 GTCTAC GT^[AC][GT]AC
ApaI 410 GGGCCC GGGCC^C
Asp700I 1057 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1216 GGTACC G^GTACC
AspHI 805 GTGCTC G[AT]GC[AT]^C
AspI 1168 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 899 TGGCCA TGG^CCA
BamHI 540 GGATCC G^GATCC
BanI 1216 GGTACC G^G[CT][GA]CC
BanII 410 GGGCCC G[GA]GC[CT]^C
BglI 1484 GCCACGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 15 GCTAAGC GC^T[ACGT]AGC
BpmI 92 CTCCAG CTGGAG(16/14)
Bpu1102I 15 GCTAAGC GC^T[ACGT]AGC
BsaAI 1327 TACGTG [CT]AC^GT[GA]
BsiEI 59 CGACCG CG[GA][CT]^CG
BsiHKAI 805 GTGCTC G[AT]GC[AT]^C
BspHI 1403 TCATGA T^CATGA
BspMI 1452 GCAGGT ACCTGC(4/8)
BsrDI 1072 CATTGC GCAATG(2/0)
BssSI 7 CTCGTG CACGAG(-5/-1)
BstAPI 773 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 15 GCTAAGC GC^T[ACGT]AGC
DrdI 1168 GACAAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1264 CTCTTC CTCTTC(1/4)
EcoNI 1401 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 40 AGCGCC [GA]GCGC^[CT]
HgaI 824 GCGTC GACGC(5/10)
HincII 1198 GTCAAC GT[CT]^[GA]AC
HindII 1198 GTCAAC GT[CT]^[GA]AC
KpnI 1216 GGTACC GGTAC^C
Ksp632I 1264 CTCTTC CTCTTC(1/4)
MluNI 899 TGGCCA TGG^CCA
MscI 899 TGGCCA TGG^CCA
PflFI 1168 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 712 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 137 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1290 CTGCAG CTGCA^G
RcaI 1403 TCATGA T^CATGA
SmaI 123 CCCGGG CCC^GGG
SpeI 163 ACTAGT A^CTAGT
SspI 418 AATATT AAT^ATT
StuI 317 AGGCCT AGG^CCT
Tth111I 1168 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 712 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 123 CCCGGG C^CCGGG
XmaI 123 CCCGGG C^CCGGG
XmnI 1057 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 27 CGCG CG^CG
692 CGCG
AciI 26 CCGC CCGC(-3/-1)
234 CCGC
AcsI 465 GAATTT [GA]^AATT[CT]
1057 GAATTT
AcyI 823 GGCGTC G[GA]^CG[CT]C
1195 GACGTC
ApoI 465 GAATTT [GA]^AATT[CT]
1057 GAATTT
AvaI 50 CCCGAG C^[CT]CG[GA]G
123 CCCGGG
AvaII 138 GGACC G^G[AT]CC
1129 GGTCC
BbsI 957 GAAGAC GAAGAC(2/6)
1177 GTCTTC
BcgI 1365 CGATTACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
641 GCACTATCATCG
BfaI 164 CTAG C^TAG
487 CTAG
BmyI 410 GGGCCC G[AGT]GC[ACT]^C
805 GTGCTC
BpuAI 957 GAAGAC GAAGAC(2/6)
1177 GTCTTC
BsaHI 823 GGCGTC G[GA]^CG[CT]C
1195 GACGTC
BseRI 877 CTCCTC GAGGAG(10/8)
571 GAGGAG
BsoBI 50 CCCGAG C^[CT]CG[GA]G
123 CCCGGG
Bsp1286I 410 GGGCCC G[AGT]GC[ACT]^C
805 GTGCTC
BstUI 27 CGCG CG^CG
692 CGCG
BstXI 599 CCACTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1132 CCAGTGCAGTGG
BstYI 540 GGATCC [GA]^GATC[CT]
923 GGATCT
CfoI 28 GCGC GCG^C
41 GCGC
DraII 137 GGGACCT [GA]G^G[ACGT]CC[CT]
409 GGGGCCC
EaeI 23 TGGCCG [CT]^GGCC[GA]
899 TGGCCA
EcoO109I 137 GGGACCT [GA]G^G[ACGT]CC[CT]
409 GGGGCCC
HhaI 28 GCGC GCG^C
41 GCGC
Hin1I 823 GGCGTC G[GA]^CG[CT]C
1195 GACGTC
HinP1I 28 GCGC G^CGC
41 GCGC
MaeI 164 CTAG C^TAG
487 CTAG
MaeII 1196 ACGT A^CGT
1328 ACGT
MflI 540 GGATCC [GA]^GATC[CT]
923 GGATCT
MseI 995 TTAA T^TAA
1280 TTAA
MslI 456 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
903 CACATGGGTG
MspA1I 291 CAGCTG C[AC]G^C[GT]G
745 CAGCTG
MvnI 27 CGCG CG^CG
692 CGCG
PleI 327 GACTC GAGTC(4/5)
750 GACTC
PvuII 291 CAGCTG CAG^CTG
745 CAGCTG
SmlI 132 CTTGAG C^T[CT][GA]AG
1256 CTCAAG
TfiI 708 GATTC G^A[AT]TC
1311 GATTC
Tru9I 995 TTAA T^TAA
1280 TTAA
Tsp45I 262 GTGAC ^GT[GC]AC
715 GTGAC
XcmI 88 CCATCTCCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
902 CCACATGGGTGCTGG
XhoII 540 GGATCC [GA]^GATC[CT]
923 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AfaI AluI AlwI AlwNI BbvI
BcnI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp509I TspRI