Restriction map for
pFN21AE3940
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI ItaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
Tru9I TseI TspRI Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 320 GAATTT [GA]^AATT[CT]
AfaI 25 GTAC GT^AC
AluI 399 AGCT AG^CT
Alw44I 97 GTGCAC G^TGCAC
AlwI 83 GATCC GGATC(4/5)
ApaLI 97 GTGCAC G^TGCAC
ApoI 320 GAATTT [GA]^AATT[CT]
AspHI 97 GTGCAC G[AT]GC[AT]^C
AspI 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 123 GGACC G^G[AT]CC
BbsI 8 GAAGAC GAAGAC(2/6)
BfaI 175 CTAG C^TAG
BmyI 97 GTGCAC G[AGT]GC[ACT]^C
BpuAI 8 GAAGAC GAAGAC(2/6)
BsaI 314 GGTCTC GGTCTC(1/5)
BsiHKAI 97 GTGCAC G[AT]GC[AT]^C
BsmBI 284 GAGACG CGTCTC(1/5)
Bsp1286I 97 GTGCAC G[AGT]GC[ACT]^C
BssKI 274 CCAGG ^CC[ACGT]GG
BstBI 5 TTCGAA TT^CGAA
BstNI 274 CCAGG CC^[AT]GG
BstYI 82 AGATCC [GA]^GATC[CT]
Cfr13I 123 GGACC G^G[ACGT]CC
DraII 122 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 271 CGGCCA [CT]^GGCC[GA]
EcoO109I 122 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 274 CCAGG ^CC[AT]GG
HapII 270 CCGG C^CGG
HpaII 270 CCGG C^CGG
MaeI 175 CTAG C^TAG
MaeII 287 ACGT A^CGT
MboII 8 GAAGA GAAGA(8/7)
MflI 82 AGATCC [GA]^GATC[CT]
MspI 270 CCGG C^CGG
MvaI 274 CCAGG CC^[AT]GG
NlaIV 123 GGACCC GG[ACGT]^[ACGT]CC
NspV 5 TTCGAA TT^CGAA
PflFI 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 49 GAGTC GAGTC(4/5)
PpuMI 122 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 44 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 25 GTAC GT^AC
Sau96I 123 GGACC G^G[ACGT]CC
ScrFI 274 CCAGG CC^[ACGT]GG
SfuI 5 TTCGAA TT^CGAA
SmlI 132 CTTGAG C^T[CT][GA]AG
StuI 276 AGGCCT AGG^CCT
TfiI 90 GAATC G^A[AT]TC
Tsp509I 321 AATT ^AATT
Tth111I 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 82 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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DdeI 280 CTCAG C^T[ACGT]AG
418 CTGAG
DpnII 83 GATC ^GATC
197 GATC
HaeIII 272 GGCC GG^CC
277 GGCC
HinfI 49 GAGTC G^A[ACGT]TC
90 GAATC
HphI 226 GGTGA GGTGA(8/7)
349 GGTGA
MaeIII 160 GTCAC ^GT[ACGT]AC
422 GTGAC
MboI 83 GATC ^GATC
197 GATC
MnlI 279 CCTC CCTC(7/6)
375 GAGG
NdeII 83 GATC ^GATC
197 GATC
Sau3AI 83 GATC ^GATC
197 GATC
SspI 165 AATATT AAT^ATT
406 AATATT
TaqI 6 TCGA T^CGA
318 TCGA
Tsp45I 160 GTCAC ^GT[GC]AC
422 GTGAC
TthHB8I 6 TCGA T^CGA
318 TCGA
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
BsmAI BsrI NlaIII