Restriction map for
pFN21AE2223
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvrII BalI BbeI
BbrPI BcgI BclI BfaI BfrI
BglI BglII BlnI BsaAI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI MaeI
MaeIII MfeI MluI MluNI MscI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 998 TCCGGA T^CCGGA
AviII 959 TGCGCA TGC^GCA
BamHI 466 GGATCC G^GATCC
BbvCI 522 CCTCAGC CCTCAGC(-5/-2)
BpmI 490 CTGGAG CTGGAG(16/14)
BsaBI 633 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 998 TCCGGA [AT]^CCGG[AT]
BseAI 998 TCCGGA T^CCGGA
BsgI 328 GTGCAG GTGCAG(16/14)
BspEI 998 TCCGGA T^CCGGA
BspMI 680 ACCTGC ACCTGC(4/8)
BsrDI 558 CATTGC GCAATG(2/0)
BstAPI 315 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 960 GCGC GCG^C
DrdI 700 GACCTGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 400 CGGCCA [CT]^GGCC[GA]
EcoNI 659 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 959 TGCGCA TGC^GCA
HhaI 960 GCGC GCG^C
HinP1I 960 GCGC G^CGC
MaeII 725 ACGT A^CGT
MamI 633 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 998 TCCGGA T^CCGGA
MseI 944 TTAA T^TAA
NcoI 695 CCATGG C^CATGG
PpuMI 451 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 144 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 321 GATGC GCATC(5/9)
SfcI 280 CTACAG C^T[GA][CT]AG
TfiI 184 GAATC G^A[AT]TC
Tru9I 944 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 398 CGCG CG^CG
783 CGCG
ApaI 26 GGGCCC GGGCC^C
690 GGGCCC
AvaI 62 CCCGGG C^[CT]CG[GA]G
179 CCCGGG
BbsI 775 GAAGAC GAAGAC(2/6)
727 GTCTTC
BciVI 167 GTATCC GTATCC(6/5)
287 GTATCC
BlpI 363 GCTCAGC GC^T[ACGT]AGC
531 GCTGAGC
Bpu1102I 363 GCTCAGC GC^T[ACGT]AGC
531 GCTGAGC
BpuAI 775 GAAGAC GAAGAC(2/6)
727 GTCTTC
BseRI 48 GAGGAG GAGGAG(10/8)
802 GAGGAG
BsmI 17 GAATGC GAATGC(1/-1)
603 GAATGC
BsoBI 62 CCCGGG C^[CT]CG[GA]G
179 CCCGGG
BstUI 398 CGCG CG^CG
783 CGCG
BstXI 122 CCATAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
138 CCACTGGACTGG
BstYI 466 GGATCC [GA]^GATC[CT]
761 AGATCC
CelII 363 GCTCAGC GC^T[ACGT]AGC
531 GCTGAGC
EarI 159 GAAGAG CTCTTC(1/4)
436 GAAGAG
FauI 781 CCCGC CCCGC(4/6)
573 GCGGG
Ksp632I 159 GAAGAG CTCTTC(1/4)
436 GAAGAG
KspI 397 CCGCGG CCGC^GG
782 CCGCGG
MflI 466 GGATCC [GA]^GATC[CT]
761 AGATCC
MslI 558 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
598 CATGAGAATG
MvnI 398 CGCG CG^CG
783 CGCG
NaeI 243 GCCGGC GCC^GGC
926 GCCGGC
NgoMIV 243 GCCGGC G^CCGGC
926 GCCGGC
PleI 859 GACTC GAGTC(4/5)
554 GAGTC
PvuII 748 CAGCTG CAG^CTG
912 CAGCTG
SacII 397 CCGCGG CCGC^GG
782 CCGCGG
SmaI 62 CCCGGG CCC^GGG
179 CCCGGG
TaqI 309 TCGA T^CGA
650 TCGA
TthHB8I 309 TCGA T^CGA
650 TCGA
XhoII 466 GGATCC [GA]^GATC[CT]
761 AGATCC
XmaCI 62 CCCGGG C^CCGGG
179 CCCGGG
XmaI 62 CCCGGG C^CCGGG
179 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AvaII BanI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr10I
Cfr13I DdeI DpnII DraII DsaI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI StyI TseI TspRI
Van91I