Restriction map for
pFN21AE3650
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SapI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 1462 GTCGAC GT^[AC][GT]AC
AlwNI 1426 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 651 GGGCCC GGGCC^C
Asp700I 222 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI 1498 CTAG C^TAG
BlpI 1084 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1084 GCTGAGC GC^T[ACGT]AGC
BsaI 495 GGTCTC GGTCTC(1/5)
BsiWI 1917 CGTACG C^GTACG
BsmAI 496 GTCTC GTCTC(1/5)
BsmI 1491 GAATGC GAATGC(1/-1)
BssSI 1855 CACGAG CACGAG(-5/-1)
BstAPI 1425 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 521 CCAGCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1084 GCTGAGC GC^T[ACGT]AGC
DrdI 364 GACCGGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1462 GTCGAC GT[CT]^[GA]AC
HindII 1462 GTCGAC GT[CT]^[GA]AC
HphI 1521 GGTGA GGTGA(8/7)
KspI 1074 CCGCGG CCGC^GG
MaeI 1498 CTAG C^TAG
MslI 1548 CACAGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 746 CCATGG C^CATGG
PvuII 401 CAGCTG CAG^CTG
SacII 1074 CCGCGG CCGC^GG
SalI 1462 GTCGAC G^TCGAC
SbfI 1515 CCTGCAGG CCTGCA^GG
SfaNI 935 GCATC GCATC(5/9)
SfiI 312 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 19 CCCGGG CCC^GGG
Sse8387I 1515 CCTGCAGG CCTGCA^GG
XcmI 407 CCAGCGACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 19 CCCGGG C^CCGGG
XmaI 19 CCCGGG C^CCGGG
XmnI 222 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BspMI 1907 ACCTGC ACCTGC(4/8)
1518 GCAGGT
BsrFI 738 GCCGGT [GA]^CCGG[CT]
1955 ACCGGC
BstEII 390 GGTAACC G^GT[ACGT]ACC
1521 GGTGACC
BstPI 390 GGTAACC G^GT[ACGT]ACC
1521 GGTGACC
BstYI 248 AGATCC [GA]^GATC[CT]
1532 AGATCC
Cfr10I 738 GCCGGT [GA]^CCGG[CT]
1955 ACCGGC
CpoI 632 CGGTCCG CG^G[AT]CCG
1357 CGGTCCG
EcoNI 528 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1995 CCTGGACGAGG
EcoO65I 390 GGTAACC G^GT[ACGT]ACC
1521 GGTGACC
MflI 248 AGATCC [GA]^GATC[CT]
1532 AGATCC
NlaIII 375 CATG CATG^
747 CATG
PflMI 1801 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1885 CCAAGGTGTGG
PstI 1423 CTGCAG CTGCA^G
1516 CTGCAG
RsrII 632 CGGTCCG CG^G[AT]CCG
1357 CGGTCCG
SacI 294 GAGCTC GAGCT^C
1117 GAGCTC
SmlI 1254 CTCAAG C^T[CT][GA]AG
1636 CTCAAG
StuI 515 AGGCCT AGG^CCT
1942 AGGCCT
TfiI 715 GATTC G^A[AT]TC
1575 GATTC
Van91I 1801 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1885 CCAAGGTGTGG
XhoII 248 AGATCC [GA]^GATC[CT]
1532 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfcI SfoI StyI TaqI
TseI Tsp45I TspRI TthHB8I