Restriction map for
pFN21AE5119
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaI HphI
KasI KspI MaeI MaeIII MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NarI NciI
NdeI NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 290 GGTACC G^GTACC
AccI 129 GTCTAC GT^[AC][GT]AC
AciI 314 GCGG CCGC(-3/-1)
AlwI 396 GATCC GGATC(4/5)
AocI 343 CCTGAGG CC^T[ACGT]AGG
Asp700I 258 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 290 GGTACC G^GTACC
BanI 290 GGTACC G^G[CT][GA]CC
BbsI 138 GAAGAC GAAGAC(2/6)
BbvCI 41 CCTCAGC CCTCAGC(-5/-2)
BbvI 433 GCAGC GCAGC(8/12)
BclI 401 TGATCA T^GATCA
BlpI 65 GCTCAGC GC^T[ACGT]AGC
BpmI 304 CTCCAG CTGGAG(16/14)
Bpu1102I 65 GCTCAGC GC^T[ACGT]AGC
BpuAI 138 GAAGAC GAAGAC(2/6)
BsaAI 74 TACGTA [CT]AC^GT[GA]
BsaI 350 GAGACC GGTCTC(1/5)
BseRI 427 GAGGAG GAGGAG(10/8)
BsmBI 337 CGTCTC CGTCTC(1/5)
BsmFI 241 GTCCC GGGAC(10/14)
BsrFI 104 GCCGGC [GA]^CCGG[CT]
BstAPI 433 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 135 TTCGAA TT^CGAA
BstYI 395 AGATCC [GA]^GATC[CT]
Bsu36I 343 CCTGAGG CC^T[ACGT]AGG
CelII 65 GCTCAGC GC^T[ACGT]AGC
Cfr10I 104 GCCGGC [GA]^CCGG[CT]
DsaI 452 CCATGG C^C[GA][CT]GG
EarI 391 GAAGAG CTCTTC(1/4)
Eco81I 343 CCTGAGG CC^T[ACGT]AGG
EcoNI 354 CCTTGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 211 GATATC GAT^ATC
FauI 314 GCGGG CCCGC(4/6)
FbaI 401 TGATCA T^GATCA
Fnu4HI 433 GCAGC GC^[ACGT]GC
FseI 103 GGCCGGCC GGCCGG^CC
HapII 105 CCGG C^CGG
HincII 222 GTCAAC GT[CT]^[GA]AC
HindII 222 GTCAAC GT[CT]^[GA]AC
HpaII 105 CCGG C^CGG
ItaI 433 GCAGC GC^[ACGT]GC
KpnI 290 GGTACC GGTAC^C
Ksp632I 391 GAAGAG CTCTTC(1/4)
MaeII 75 ACGT A^CGT
MflI 395 AGATCC [GA]^GATC[CT]
MspI 105 CCGG C^CGG
NaeI 104 GCCGGC GCC^GGC
NcoI 452 CCATGG C^CATGG
NgoMIV 104 GCCGGC G^CCGGC
NspV 135 TTCGAA TT^CGAA
PpuMI 329 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 331 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 135 TTCGAA TT^CGAA
SnaBI 74 TACGTA TAC^GTA
TseI 433 GCAGC G^C[AT]GC
XcmI 161 CCAAGGTCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 395 AGATCC [GA]^GATC[CT]
XmnI 258 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 269 AGCT AG^CT
435 AGCT
AspHI 63 GTGCTC G[AT]GC[AT]^C
94 GTGCTC
AvaII 165 GGTCC G^G[AT]CC
330 GGACC
BmyI 63 GTGCTC G[AGT]GC[ACT]^C
94 GTGCTC
BsaBI 273 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
396 GATCCTGATC
BsiHKAI 63 GTGCTC G[AT]GC[AT]^C
94 GTGCTC
Bsp1286I 63 GTGCTC G[AGT]GC[ACT]^C
94 GTGCTC
BsrI 142 ACTGG ACTGG(1/-1)
193 ACTGG
BssKI 282 CCAGG ^CC[ACGT]GG
306 CCAGG
BstNI 282 CCAGG CC^[AT]GG
306 CCAGG
BstXI 168 CCATCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
267 CCAGCTGATTGG
Cac8I 104 GCCGGC GC[ACGT]^[ACGT]GC
146 GCAAGC
DraII 37 GGGGCCT [GA]G^G[ACGT]CC[CT]
329 AGGACCT
EaeI 106 CGGCCA [CT]^GGCC[GA]
153 CGGCCA
EcoO109I 37 GGGGCCT [GA]G^G[ACGT]CC[CT]
329 AGGACCT
EcoRII 282 CCAGG ^CC[AT]GG
306 CCAGG
MamI 273 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
396 GATCCTGATC
MvaI 282 CCAGG CC^[AT]GG
306 CCAGG
NlaIV 37 GGGGCC GG[ACGT]^[ACGT]CC
290 GGTACC
PvuII 268 CAGCTG CAG^CTG
434 CAGCTG
ScrFI 282 CCAGG CC^[ACGT]GG
306 CCAGG
SfaNI 31 GATGC GCATC(5/9)
278 GCATC
TspRI 61 CAGTG CA[GC]TG[ACGT][ACGT]^
299 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI BsaJI BsiYI BslI BsmAI
BstF5I Cfr13I DdeI DpnII EcoT14I
FokI HaeIII MboI MboII MnlI
MspA1I MwoI NdeII NlaIII RsaI
Sau3AI Sau96I StyI TaqI TthHB8I