Restriction map for
pFN21AE1486
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AhdI AlwI AocI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI SacI
SacII SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 184 GACGTC GACGT^C
AccII 2202 CGCG CG^CG
AgeI 2651 ACCGGT A^CCGGT
Alw44I 2380 GTGCAC G^TGCAC
Aor51HI 1998 AGCGCT AGC^GCT
ApaLI 2380 GTGCAC G^TGCAC
AspI 2437 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2207 TGCGCA TGC^GCA
BalI 1091 TGGCCA TGG^CCA
BbeI 852 GGCGCC GGCGC^C
BsiEI 1857 CGGTCG CG[GA][CT]^CG
BsmI 326 GCATTC GAATGC(1/-1)
BspDI 423 ATCGAT AT^CGAT
BsrFI 2651 ACCGGT [GA]^CCGG[CT]
BstUI 2202 CGCG CG^CG
Cfr10I 2651 ACCGGT [GA]^CCGG[CT]
ClaI 423 ATCGAT AT^CGAT
CpoI 1424 CGGACCG CG^G[AT]CCG
EaeI 1091 TGGCCA [CT]^GGCC[GA]
Eco47III 1998 AGCGCT AGC^GCT
EcoNI 509 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 2207 TGCGCA TGC^GCA
KasI 852 GGCGCC G^GCGCC
MluNI 1091 TGGCCA TGG^CCA
MscI 1091 TGGCCA TGG^CCA
MseI 1031 TTAA T^TAA
MvnI 2202 CGCG CG^CG
NarI 852 GGCGCC GG^CGCC
NcoI 1685 CCATGG C^CATGG
NheI 396 GCTAGC G^CTAGC
PaeR7I 1572 CTCGAG C^TCGAG
PflFI 2437 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 753 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2651 ACCGGT A^CCGGT
PshAI 184 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 266 CTGCAG CTGCA^G
RsrII 1424 CGGACCG CG^G[AT]CCG
SalI 1813 GTCGAC G^TCGAC
ScaI 1615 AGTACT AGT^ACT
SexAI 2147 ACCAGGT A^CC[AT]GGT
SfcI 266 CTGCAG C^T[GA][CT]AG
SfoI 852 GGCGCC GGC^GCC
Tru9I 1031 TTAA T^TAA
Tth111I 2437 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 753 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 432 TCTAGA T^CTAGA
XhoI 1572 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 1084 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1199 CAGACACTG
ApaI 696 GGGCCC GGGCC^C
1194 GGGCCC
Asp700I 2016 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2467 GAAGTCCTTC
AspHI 2380 GTGCAC G[AT]GC[AT]^C
2416 GTGCTC
BcnI 1428 CCGGG CC^[GC]GG
2159 CCGGG
BfaI 397 CTAG C^TAG
433 CTAG
BglII 1919 AGATCT A^GATCT
1958 AGATCT
BsaAI 1303 TACGTG [CT]AC^GT[GA]
2377 TACGTG
BsaI 1564 GAGACC GGTCTC(1/5)
2353 GAGACC
BsaWI 2517 ACCGGA [AT]^CCGG[AT]
2651 ACCGGT
BsiHKAI 2380 GTGCAC G[AT]GC[AT]^C
2416 GTGCTC
BstAPI 1228 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2560 GCACTGCTTGC
BstXI 2267 CCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2357 CCAACGAAGTGG
BstYI 1919 AGATCT [GA]^GATC[CT]
1958 AGATCT
HgaI 874 GACGC GACGC(5/10)
1952 GCGTC
HincII 1813 GTCGAC GT[CT]^[GA]AC
2143 GTCAAC
HindII 1813 GTCGAC GT[CT]^[GA]AC
2143 GTCAAC
MaeI 397 CTAG C^TAG
433 CTAG
MflI 1919 AGATCT [GA]^GATC[CT]
1958 AGATCT
NciI 1428 CCGGG CC^[GC]GG
2159 CCGGG
NspI 669 GCATGT [GA]CATG^[CT]
1469 GCATGT
SmlI 1572 CTCGAG C^T[CT][GA]AG
1759 CTCAAG
StuI 1266 AGGCCT AGG^CCT
2670 AGGCCT
Tsp509I 680 AATT ^AATT
1866 AATT
XhoII 1919 AGATCT [GA]^GATC[CT]
1958 AGATCT
XmnI 2016 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2467 GAAGTCCTTC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I