Restriction map for
pFN21AE9615
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AcyI 118 GGCGTC G[GA]^CG[CT]C
Alw44I 244 GTGCAC G^TGCAC
AlwNI 664 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 672 GGGCCC GGGCC^C
ApaLI 244 GTGCAC G^TGCAC
Asp700I 516 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 244 GTGCAC G[AT]GC[AT]^C
BalI 584 TGGCCA TGG^CCA
BamHI 51 GGATCC G^GATCC
BanI 93 GGTGCC G^G[CT][GA]CC
BfaI 145 CTAG C^TAG
BglI 232 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 875 AGATCT A^GATCT
BpmI 804 CTCCAG CTGGAG(16/14)
BsaBI 876 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 118 GGCGTC G[GA]^CG[CT]C
BsaI 759 GGTCTC GGTCTC(1/5)
BseRI 643 GAGGAG GAGGAG(10/8)
BsiHKAI 244 GTGCAC G[AT]GC[AT]^C
BsmAI 760 GTCTC GTCTC(1/5)
Bsp1407I 2 TGTACA T^GTACA
BspHI 317 TCATGA T^CATGA
BspMI 930 GCAGGT ACCTGC(4/8)
BsrDI 299 GCAATG GCAATG(2/0)
BsrFI 90 GCCGGT [GA]^CCGG[CT]
BsrGI 2 TGTACA T^GTACA
Cfr10I 90 GCCGGT [GA]^CCGG[CT]
DsaI 331 CCACGG C^C[GA][CT]GG
EcoNI 786 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 831 GATATC GAT^ATC
EcoT14I 791 CCAAGG C^C[AT][AT]GG
HgaI 119 GCGTC GACGC(5/10)
Hin1I 118 GGCGTC G[GA]^CG[CT]C
HphI 423 GGTGA GGTGA(8/7)
MaeI 145 CTAG C^TAG
MaeIII 631 GTCAC ^GT[ACGT]AC
MamI 876 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 584 TGGCCA TGG^CCA
MscI 584 TGGCCA TGG^CCA
MslI 975 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 663 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 928 CTGCAG CTGCA^G
RcaI 317 TCATGA T^CATGA
SbfI 927 CCTGCAGG CCTGCA^GG
SexAI 419 ACCAGGT A^CC[AT]GGT
SfaNI 766 GATGC GCATC(5/9)
SfcI 928 CTGCAG C^T[GA][CT]AG
SmaI 209 CCCGGG CCC^GGG
SmlI 340 CTCAAG C^T[CT][GA]AG
SphI 956 GCATGC GCATG^C
Sse8387I 927 CCTGCAGG CCTGCA^GG
SspBI 2 TGTACA T^GTACA
StuI 57 AGGCCT AGG^CCT
StyI 791 CCAAGG C^C[AT][AT]GG
TfiI 987 GATTC G^A[AT]TC
Tsp45I 631 GTCAC ^GT[GC]AC
Tsp509I 1025 AATT ^AATT
Van91I 663 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
XmnI 516 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 286 CGCG CG^CG
953 CGCG
AocI 703 CCTGAGG CC^T[ACGT]AGG
994 CCTCAGG
AvaI 129 CTCGGG C^[CT]CG[GA]G
209 CCCGGG
BbsI 709 GAAGAC GAAGAC(2/6)
121 GTCTTC
BpuAI 709 GAAGAC GAAGAC(2/6)
121 GTCTTC
BsoBI 129 CTCGGG C^[CT]CG[GA]G
209 CCCGGG
BstUI 286 CGCG CG^CG
953 CGCG
BstXI 348 CCAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
545 CCAACATCCTGG
BstYI 51 GGATCC [GA]^GATC[CT]
875 AGATCT
Bsu36I 703 CCTGAGG CC^T[ACGT]AGG
994 CCTCAGG
CfoI 103 GCGC GCG^C
954 GCGC
EaeI 584 TGGCCA [CT]^GGCC[GA]
935 TGGCCG
EarI 313 CTCTTC CTCTTC(1/4)
166 GAAGAG
Eco81I 703 CCTGAGG CC^T[ACGT]AGG
994 CCTCAGG
FauI 72 GCGGG CCCGC(4/6)
456 GCGGG
HhaI 103 GCGC GCG^C
954 GCGC
HinP1I 103 GCGC G^CGC
954 GCGC
Ksp632I 313 CTCTTC CTCTTC(1/4)
166 GAAGAG
MflI 51 GGATCC [GA]^GATC[CT]
875 AGATCT
MspA1I 398 CAGCTG C[AC]G^C[GT]G
719 CAGCTG
MvnI 286 CGCG CG^CG
953 CGCG
NspI 63 GCATGT [GA]CATG^[CT]
956 GCATGC
PpuMI 572 GGGACCT [GA]G^G[AT]CC[CT]
665 AGGTCCT
PvuII 398 CAGCTG CAG^CTG
719 CAGCTG
XhoII 51 GGATCC [GA]^GATC[CT]
875 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI TseI
TspRI