Restriction map for
pFN21AE4485
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspMI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FseI FspI HapII Hin1I HindIII
HpaII HphI KasI KpnI MfeI
MflI MluI MluNI MroI MscI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I StuI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 411 ACATGT A^C[GA][CT]GT
AocI 484 CCTGAGG CC^T[ACGT]AGG
AvaII 805 GGACC G^G[AT]CC
BcgI 1449 GCATAATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 528 GGATAC GTATCC(6/5)
BclI 431 TGATCA T^GATCA
BsaAI 185 TACGTG [CT]AC^GT[GA]
BsaBI 1174 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 281 GAGACC GGTCTC(1/5)
BsmBI 589 CGTCTC CGTCTC(1/5)
BsmFI 744 GTCCC GGGAC(10/14)
Bsp1407I 388 TGTACA T^GTACA
BspHI 1256 TCATGA T^CATGA
BspLU11I 411 ACATGT A^CATGT
BsrBI 818 GAGCGG CCGCTC(-3/-3)
BsrGI 388 TGTACA T^GTACA
BssSI 1478 CTCGTG CACGAG(-5/-1)
Bsu36I 484 CCTGAGG CC^T[ACGT]AGG
Cfr13I 805 GGACC G^G[ACGT]CC
DraI 718 TTTAAA TTT^AAA
DrdI 541 GACAACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 484 CCTGAGG CC^T[ACGT]AGG
EcoT14I 926 CCATGG C^C[AT][AT]GG
EcoT22I 728 ATGCAT ATGCA^T
FauI 42 GCGGG CCCGC(4/6)
FbaI 431 TGATCA T^GATCA
HaeIII 482 GGCC GG^CC
HgaI 55 GACGC GACGC(5/10)
HincII 520 GTTAAC GT[CT]^[GA]AC
HindII 520 GTTAAC GT[CT]^[GA]AC
HpaI 520 GTTAAC GTT^AAC
KspI 40 CCGCGG CCGC^GG
MamI 1174 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 926 CCATGG C^CATGG
NsiI 728 ATGCAT ATGCA^T
NspI 411 ACATGT [GA]CATG^[CT]
PacI 628 TTAATTAA TTAAT^TAA
PstI 460 CTGCAG CTGCA^G
RcaI 1256 TCATGA T^CATGA
SacI 1283 GAGCTC GAGCT^C
SacII 40 CCGCGG CCGC^GG
SapI 509 GAAGAGC GCTCTTC(1/4)
Sau96I 805 GGACC G^G[ACGT]CC
SfcI 460 CTGCAG C^T[GA][CT]AG
SpeI 571 ACTAGT A^CTAGT
SspBI 388 TGTACA T^GTACA
StyI 926 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 41 CGCG CG^CG
57 CGCG
AlwI 1300 GATCC GGATC(4/5)
1483 GATCC
AspHI 1104 GAGCAC G[AT]GC[AT]^C
1283 GAGCTC
BanII 1283 GAGCTC G[GA]GC[CT]^C
1385 GAGCCC
BfaI 572 CTAG C^TAG
1036 CTAG
BpmI 592 CTCCAG CTGGAG(16/14)
1073 CTGGAG
BsaJI 40 CCGCGG C^C[ACGT][ACGT]GG
926 CCATGG
BsiHKAI 1104 GAGCAC G[AT]GC[AT]^C
1283 GAGCTC
BsiYI 459 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1393 CCTCATGCTGG
BslI 459 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1393 CCTCATGCTGG
BstF5I 342 GGATG GGATG(2/0)
468 GGATG
BstUI 41 CGCG CG^CG
57 CGCG
CfoI 8 GCGC GCG^C
14 GCGC
DsaI 40 CCGCGG C^C[GA][CT]GG
926 CCATGG
FokI 342 GGATG GGATG(9/13)
468 GGATG
HaeII 7 AGCGCC [GA]GCGC^[CT]
13 AGCGCC
HhaI 8 GCGC GCG^C
14 GCGC
HinP1I 8 GCGC G^CGC
14 GCGC
MaeI 572 CTAG C^TAG
1036 CTAG
MaeIII 408 GTTAC ^GT[ACGT]AC
1426 GTTAC
MslI 870 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1396 CATGCTGGTG
MvnI 41 CGCG CG^CG
57 CGCG
PvuII 463 CAGCTG CAG^CTG
674 CAGCTG
SfaNI 472 GATGC GCATC(5/9)
602 GCATC
TaqI 126 TCGA T^CGA
1079 TCGA
TfiI 369 GAATC G^A[AT]TC
1369 GATTC
TthHB8I 126 TCGA T^CGA
1079 TCGA
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BbsI BbvI BmyI BpuAI
BseRI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I DdeI DpnII EarI
EcoRII Fnu4HI HinfI ItaI Ksp632I
MaeII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI ScrFI
SspI Tru9I TseI Tsp509I TspRI
XmnI