Restriction map for
pFN21AE4578
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AocI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbeI BbrPI BciVI BfaI BfrI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmI BspDI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MunI
NarI NdeI NheI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 944 TCCGGA T^CCGGA
AcyI 430 GGCGTC G[GA]^CG[CT]C
AhdI 1066 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1182 GTGCAC G^TGCAC
Aor51HI 767 AGCGCT AGC^GCT
ApaLI 1182 GTGCAC G^TGCAC
AspEI 1066 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1645 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 916 GTCTTC GAAGAC(2/6)
BbvCI 1745 CCTCAGC CCTCAGC(-5/-2)
BcgI 1248 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 515 TGATCA T^GATCA
BglII 647 AGATCT A^GATCT
BpuAI 916 GTCTTC GAAGAC(2/6)
BsaHI 430 GGCGTC G[GA]^CG[CT]C
BsaI 1471 GAGACC GGTCTC(1/5)
BsaWI 944 TCCGGA [AT]^CCGG[AT]
BseAI 944 TCCGGA T^CCGGA
BsmBI 2263 GAGACG CGTCTC(1/5)
BspEI 944 TCCGGA T^CCGGA
BspLU11I 2060 ACATGT A^CATGT
BsrDI 1190 GCAATG GCAATG(2/0)
BssSI 1246 CACGAG CACGAG(-5/-1)
BstAPI 242 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 243 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1066 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 767 AGCGCT AGC^GCT
EcoRI 675 GAATTC G^AATTC
FbaI 515 TGATCA T^GATCA
HaeII 767 AGCGCT [GA]GCGC^[CT]
HgaI 431 GCGTC GACGC(5/10)
Hin1I 430 GGCGTC G[GA]^CG[CT]C
MluI 1853 ACGCGT A^CGCGT
MroI 944 TCCGGA T^CCGGA
NaeI 1781 GCCGGC GCC^GGC
NgoMIV 1781 GCCGGC G^CCGGC
NotI 1316 GCGGCCGC GC^GGCCGC
PaeR7I 936 CTCGAG C^TCGAG
PflFI 1645 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1228 GAGTC GAGTC(4/5)
ScaI 104 AGTACT AGT^ACT
SmlI 936 CTCGAG C^T[CT][GA]AG
SphI 409 GCATGC GCATG^C
StuI 7 AGGCCT AGG^CCT
Tth111I 1645 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 936 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 499 AAATTT [GA]^AATT[CT]
675 GAATTC
AflIII 1853 ACGCGT A^C[GA][CT]GT
2060 ACATGT
AlwNI 1124 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1962 CAGCTTCTG
ApaI 355 GGGCCC GGGCC^C
831 GGGCCC
ApoI 499 AAATTT [GA]^AATT[CT]
675 GAATTC
BcnI 210 CCGGG CC^[GC]GG
291 CCGGG
BsaAI 304 TACGTG [CT]AC^GT[GA]
613 TACGTG
Bsp1407I 737 TGTACA T^GTACA
2228 TGTACA
BspHI 1514 TCATGA T^CATGA
2186 TCATGA
BsrGI 737 TGTACA T^GTACA
2228 TGTACA
EarI 189 GAAGAG CTCTTC(1/4)
2260 GAAGAG
EcoNI 752 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2124 CCTGTTCCAGG
HincII 1495 GTCAAC GT[CT]^[GA]AC
2092 GTTGAC
HindII 1495 GTCAAC GT[CT]^[GA]AC
2092 GTTGAC
Ksp632I 189 GAAGAG CTCTTC(1/4)
2260 GAAGAG
MseI 2141 TTAA T^TAA
2299 TTAA
NciI 210 CCGGG CC^[GC]GG
291 CCGGG
PpuMI 1064 GGGACCT [GA]G^G[AT]CC[CT]
1366 GGGTCCC
RcaI 1514 TCATGA T^CATGA
2186 TCATGA
SspBI 737 TGTACA T^GTACA
2228 TGTACA
Tru9I 2141 TTAA T^TAA
2299 TTAA
XcmI 818 CCAGCTGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1961 CCAGCTTCTGCTTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanI
BanII BbvI BglI BmyI BpmI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII