Restriction map for
pFN21AE1091
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BciVI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 55 GTCTAC GT^[AC][GT]AC
AfaI 189 GTAC GT^AC
Aor51HI 368 AGCGCT AGC^GCT
ApaI 167 GGGCCC GGGCC^C
Asp700I 915 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 940 GAGCTC G[AT]GC[AT]^C
BalI 554 TGGCCA TGG^CCA
BbvCI 537 CCTCAGC CCTCAGC(-5/-2)
BcgI 790 GCAGTGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 267 TGATCA T^GATCA
BfaI 436 CTAG C^TAG
BglII 149 AGATCT A^GATCT
BsiHKAI 940 GAGCTC G[AT]GC[AT]^C
BsmAI 638 GTCTC GTCTC(1/5)
Bsp1407I 188 TGTACA T^GTACA
BsrBI 694 GAGCGG CCGCTC(-3/-3)
BsrFI 926 GCCGGC [GA]^CCGG[CT]
BsrGI 188 TGTACA T^GTACA
BstYI 149 AGATCT [GA]^GATC[CT]
Cfr10I 926 GCCGGC [GA]^CCGG[CT]
DdeI 538 CTCAG C^T[ACGT]AG
DraII 167 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 722 CCGTGG C^C[GA][CT]GG
Eco47III 368 AGCGCT AGC^GCT
EcoO109I 167 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 261 CCAAGG C^C[AT][AT]GG
FbaI 267 TGATCA T^GATCA
HaeII 368 AGCGCT [GA]GCGC^[CT]
MaeI 436 CTAG C^TAG
MflI 149 AGATCT [GA]^GATC[CT]
MluNI 554 TGGCCA TGG^CCA
MscI 554 TGGCCA TGG^CCA
NaeI 926 GCCGGC GCC^GGC
NgoMIV 926 GCCGGC G^CCGGC
RsaI 189 GTAC GT^AC
SacI 940 GAGCTC GAGCT^C
SfcI 946 CTACAG C^T[GA][CT]AG
SfiI 711 GGCCCGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 925 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 188 TGTACA T^GTACA
StuI 830 AGGCCT AGG^CCT
StyI 261 CCAAGG C^C[AT][AT]GG
TaqI 799 TCGA T^CGA
Tsp45I 463 GTGAC ^GT[GC]AC
TthHB8I 799 TCGA T^CGA
XhoII 149 AGATCT [GA]^GATC[CT]
XmnI 915 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 61 CGCG CG^CG
857 CGCG
AcyI 801 GACGCC G[GA]^CG[CT]C
808 GGCGTC
AvaII 303 GGTCC G^G[AT]CC
888 GGACC
BanII 167 GGGCCC G[GA]GC[CT]^C
940 GAGCTC
BmyI 167 GGGCCC G[AGT]GC[ACT]^C
940 GAGCTC
BsaHI 801 GACGCC G[GA]^CG[CT]C
808 GGCGTC
BsiEI 182 CGGCCG CG[GA][CT]^CG
859 CGGCCG
Bsp1286I 167 GGGCCC G[AGT]GC[ACT]^C
940 GAGCTC
BstAPI 75 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
521 GCACTGTCTGC
BstUI 61 CGCG CG^CG
857 CGCG
CfoI 369 GCGC GCG^C
795 GCGC
DpnII 150 GATC ^GATC
268 GATC
EagI 182 CGGCCG C^GGCCG
859 CGGCCG
EclXI 182 CGGCCG C^GGCCG
859 CGGCCG
Eco52I 182 CGGCCG C^GGCCG
859 CGGCCG
HgaI 801 GACGC GACGC(5/10)
809 GCGTC
HhaI 369 GCGC GCG^C
795 GCGC
Hin1I 801 GACGCC G[GA]^CG[CT]C
808 GGCGTC
HinP1I 369 GCGC G^CGC
795 GCGC
MaeII 157 ACGT A^CGT
411 ACGT
MaeIII 463 GTGAC ^GT[ACGT]AC
515 GTAAC
MboI 150 GATC ^GATC
268 GATC
MvnI 61 CGCG CG^CG
857 CGCG
NdeII 150 GATC ^GATC
268 GATC
PflMI 24 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
387 CCACCCGCTGG
Sau3AI 150 GATC ^GATC
268 GATC
Van91I 24 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
387 CCACCCGCTGG
XcmI 690 CCAGGAGCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
875 CCAACAGCGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwNI BbvI BcnI
BglI BsaJI BsgI BsiYI BslI
BsmFI BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I EaeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI Ksp632I
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI TspRI