Restriction map for
pFN21AE6730
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp718I AviII AvrII
BbsI BclI BfrI BglII BlnI
BpuAI BsaBI BsiWI BsmI BspDI
BspLU11I BsrDI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
DraIII DrdI Eco47III EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AflIII 1583 ACACGT A^C[GA][CT]GT
AhdI 955 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1327 GTGCAC G^TGCAC
AocI 436 CCTGAGG CC^T[ACGT]AGG
ApaLI 1327 GTGCAC G^TGCAC
Asp700I 157 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 955 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 925 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2237 TGGCCA TGG^CCA
BbeI 812 GGCGCC GGCGC^C
BbrPI 1584 CACGTG CAC^GTG
BcgI 1117 GCAACCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2400 GGATAC GTATCC(6/5)
BlpI 57 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 57 GCTGAGC GC^T[ACGT]AGC
BsmBI 454 GAGACG CGTCTC(1/5)
Bsp1407I 2009 TGTACA T^GTACA
BspHI 1763 TCATGA T^CATGA
BsrBI 937 GAGCGG CCGCTC(-3/-3)
BsrGI 2009 TGTACA T^GTACA
BssSI 1125 CTCGTG CACGAG(-5/-1)
Bsu36I 436 CCTGAGG CC^T[ACGT]AGG
CelII 57 GCTGAGC GC^T[ACGT]AGC
Eam1105I 955 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1339 GAAGAG CTCTTC(1/4)
Eco81I 436 CCTGAGG CC^T[ACGT]AGG
HindIII 2302 AAGCTT A^AGCTT
KasI 812 GGCGCC G^GCGCC
Ksp632I 1339 GAAGAG CTCTTC(1/4)
MluNI 2237 TGGCCA TGG^CCA
MscI 2237 TGGCCA TGG^CCA
MseI 1012 TTAA T^TAA
NarI 812 GGCGCC GG^CGCC
NcoI 549 CCATGG C^CATGG
NheI 231 GCTAGC G^CTAGC
NspI 1391 ACATGC [GA]CATG^[CT]
PaeR7I 1873 CTCGAG C^TCGAG
PflFI 925 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1584 CACGTG CAC^GTG
PmlI 1584 CACGTG CAC^GTG
RcaI 1763 TCATGA T^CATGA
SacI 2158 GAGCTC GAGCT^C
SfiI 138 GGCCTCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 812 GGCGCC GGC^GCC
SmlI 1873 CTCGAG C^T[CT][GA]AG
SspBI 2009 TGTACA T^GTACA
StuI 1533 AGGCCT AGG^CCT
Tru9I 1012 TTAA T^TAA
Tsp509I 51 AATT ^AATT
Tth111I 925 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1873 CTCGAG C^TCGAG
XmnI 157 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 219 TCCGGA T^CCGGA
1605 TCCGGA
BamHI 71 GGATCC G^GATCC
1185 GGATCC
BpmI 1147 CTGGAG CTGGAG(16/14)
1617 CTGGAG
BsaAI 970 TACGTG [CT]AC^GT[GA]
1584 CACGTG
BsaWI 219 TCCGGA [AT]^CCGG[AT]
1605 TCCGGA
BseAI 219 TCCGGA T^CCGGA
1605 TCCGGA
BsiEI 245 CGGCCG CG[GA][CT]^CG
541 CGGCCG
BspEI 219 TCCGGA T^CCGGA
1605 TCCGGA
BstEII 498 GGTGACC G^GT[ACGT]ACC
2082 GGTCACC
BstPI 498 GGTGACC G^GT[ACGT]ACC
2082 GGTCACC
BstXI 686 CCAGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2354 CCATCACCGTGG
EagI 245 CGGCCG C^GGCCG
541 CGGCCG
EclXI 245 CGGCCG C^GGCCG
541 CGGCCG
Eco52I 245 CGGCCG C^GGCCG
541 CGGCCG
EcoO65I 498 GGTGACC G^GT[ACGT]ACC
2082 GGTCACC
EcoT14I 429 CCTTGG C^C[AT][AT]GG
549 CCATGG
FseI 246 GGCCGGCC GGCCGG^CC
542 GGCCGGCC
MboII 1339 GAAGA GAAGA(8/7)
2048 TCTTC
MroI 219 TCCGGA T^CCGGA
1605 TCCGGA
PflMI 109 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
748 CCAGAGCCTGG
PstI 622 CTGCAG CTGCA^G
1498 CTGCAG
StyI 429 CCTTGG C^C[AT][AT]GG
549 CCATGG
TfiI 607 GATTC G^A[AT]TC
913 GATTC
Van91I 109 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
748 CCAGAGCCTGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII
XmaCI XmaI