Restriction map for
pFN21AE6317
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MspA1I MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuII
RcaI RsrII SacI SacII SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 601 GTCGAC GT^[AC][GT]AC
AccII 694 CGCG CG^CG
AcyI 403 GGCGCC G[GA]^CG[CT]C
AflIII 890 ACATGT A^C[GA][CT]GT
ApaI 559 GGGCCC GGGCC^C
AvaI 556 CTCGGG C^[CT]CG[GA]G
BalI 264 TGGCCA TGG^CCA
BbeI 403 GGCGCC GGCGC^C
BsaHI 403 GGCGCC G[GA]^CG[CT]C
BsaI 897 GGTCTC GGTCTC(1/5)
BsmBI 678 CGTCTC CGTCTC(1/5)
BsoBI 556 CTCGGG C^[CT]CG[GA]G
BspLU11I 890 ACATGT A^CATGT
BsrFI 448 GCCGGC [GA]^CCGG[CT]
BsrI 131 ACTGG ACTGG(1/-1)
BssHII 693 GCGCGC G^CGCGC
BstAPI 919 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 694 CGCG CG^CG
Cfr10I 448 GCCGGC [GA]^CCGG[CT]
DdeI 615 CTCAG C^T[ACGT]AG
EagI 422 CGGCCG C^GGCCG
EclXI 422 CGGCCG C^GGCCG
Eco52I 422 CGGCCG C^GGCCG
FauI 60 CCCGC CCCGC(4/6)
HaeII 403 GGCGCC [GA]GCGC^[CT]
HgaI 928 GCGTC GACGC(5/10)
Hin1I 403 GGCGCC G[GA]^CG[CT]C
HincII 601 GTCGAC GT[CT]^[GA]AC
HindII 601 GTCGAC GT[CT]^[GA]AC
KasI 403 GGCGCC G^GCGCC
MaeII 311 ACGT A^CGT
MluNI 264 TGGCCA TGG^CCA
MscI 264 TGGCCA TGG^CCA
MvnI 694 CGCG CG^CG
NaeI 448 GCCGGC GCC^GGC
NarI 403 GGCGCC GG^CGCC
NgoMIV 448 GCCGGC G^CCGGC
PflMI 390 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 594 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 123 CTGCAG CTGCA^G
PvuI 744 CGATCG CGAT^CG
SalI 601 GTCGAC G^TCGAC
SapI 205 GAAGAGC GCTCTTC(1/4)
SexAI 92 ACCTGGT A^CC[AT]GGT
SfcI 123 CTGCAG C^T[GA][CT]AG
SfoI 403 GGCGCC GGC^GCC
SmlI 811 CTCAAG C^T[CT][GA]AG
SphI 29 GCATGC GCATG^C
TspRI 192 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 390 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 252 CCACGACCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BanI 403 GGCGCC G^G[CT][GA]CC
826 GGTGCC
BanII 559 GGGCCC G[GA]GC[CT]^C
735 GGGCTC
BcnI 436 CCGGG CC^[GC]GG
633 CCGGG
BpmI 570 CTCCAG CTGGAG(16/14)
730 CTCCAG
BsiEI 422 CGGCCG CG[GA][CT]^CG
744 CGATCG
BsmAI 679 GTCTC GTCTC(1/5)
898 GTCTC
BsmI 672 GAATGC GAATGC(1/-1)
68 GCATTC
DpnII 627 GATC ^GATC
745 GATC
EarI 118 CTCTTC CTCTTC(1/4)
205 GAAGAG
HphI 90 TCACC GGTGA(8/7)
863 TCACC
Ksp632I 118 CTCTTC CTCTTC(1/4)
205 GAAGAG
MaeIII 755 GTGAC ^GT[ACGT]AC
862 GTCAC
MboI 627 GATC ^GATC
745 GATC
MslI 162 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
253 CACGACCATG
NciI 436 CCGGG CC^[GC]GG
633 CCGGG
NcoI 174 CCATGG C^CATGG
395 CCATGG
NdeII 627 GATC ^GATC
745 GATC
PleI 568 GACTC GAGTC(4/5)
807 GACTC
Sau3AI 627 GATC ^GATC
745 GATC
SfaNI 219 GATGC GCATC(5/9)
659 GCATC
TaqI 566 TCGA T^CGA
602 TCGA
TfiI 343 GATTC G^A[AT]TC
490 GAATC
Tsp45I 755 GTGAC ^GT[GC]AC
862 GTCAC
TthHB8I 566 TCGA T^CGA
602 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BbsI BbvI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboII MnlI
MspI MvaI MwoI NlaIII NlaIV
NspI PpuMI RsaI Sau96I ScrFI
StyI TseI