Restriction map for
pFN21AE3613
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI MaeI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 136 GTAGAC GT^[AC][GT]AC
AlwNI 303 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 8 GGGCCC GGGCC^C
AspHI 112 GAGCTC G[AT]GC[AT]^C
AvaI 5 CCCGGG C^[CT]CG[GA]G
BanI 97 GGTGCC G^G[CT][GA]CC
BseRI 103 CTCCTC GAGGAG(10/8)
BsgI 213 CTGCAC GTGCAG(16/14)
BsiHKAI 112 GAGCTC G[AT]GC[AT]^C
BsmAI 157 GAGAC GTCTC(1/5)
BsmBI 157 GAGACG CGTCTC(1/5)
BsoBI 5 CCCGGG C^[CT]CG[GA]G
BspHI 65 TCATGA T^CATGA
BstEII 384 GGTGACC G^GT[ACGT]ACC
BstF5I 351 CATCC GGATG(2/0)
BstPI 384 GGTGACC G^GT[ACGT]ACC
DsaI 25 CCGCGG C^C[GA][CT]GG
EarI 61 CTCTTC CTCTTC(1/4)
EcoO65I 384 GGTGACC G^GT[ACGT]ACC
FokI 351 CATCC GGATG(9/13)
HgaI 159 GACGC GACGC(5/10)
Ksp632I 61 CTCTTC CTCTTC(1/4)
KspI 25 CCGCGG CCGC^GG
MslI 378 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 25 CCGCGG C[AC]G^C[GT]G
PpuMI 335 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 65 TCATGA T^CATGA
SacI 112 GAGCTC GAGCT^C
SacII 25 CCGCGG CCGC^GG
SfaNI 350 GCATC GCATC(5/9)
SfcI 195 CTACAG C^T[GA][CT]AG
SmaI 5 CCCGGG CCC^GGG
XcmI 254 CCAATGCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AccII 26 CGCG CG^CG
31 CGCG
BanII 8 GGGCCC G[GA]GC[CT]^C
112 GAGCTC
BsaJI 5 CCCGGG C^C[ACGT][ACGT]GG
25 CCGCGG
BsmFI 335 GGGAC GGGAC(10/14)
373 GTCCC
BstUI 26 CGCG CG^CG
31 CGCG
CfoI 30 GCGC GCG^C
426 GCGC
DdeI 318 CTCAG C^T[ACGT]AG
431 CTGAG
DraII 8 GGGCCCT [GA]G^G[ACGT]CC[CT]
335 GGGACCC
EaeI 206 TGGCCG [CT]^GGCC[GA]
291 TGGCCG
EcoO109I 8 GGGCCCT [GA]G^G[ACGT]CC[CT]
335 GGGACCC
HhaI 30 GCGC GCG^C
426 GCGC
HinP1I 30 GCGC G^CGC
426 GCGC
MboII 62 TCTTC GAAGA(8/7)
275 TCTTC
MvnI 26 CGCG CG^CG
31 CGCG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I EcoRII
FauI Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeIII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI TseI Tsp45I TspRI