################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 136 GTAGAC GT^[AC][GT]AC AlwNI 303 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 8 GGGCCC GGGCC^C AspHI 112 GAGCTC G[AT]GC[AT]^C AvaI 5 CCCGGG C^[CT]CG[GA]G BanI 97 GGTGCC G^G[CT][GA]CC BseRI 103 CTCCTC GAGGAG(10/8) BsgI 213 CTGCAC GTGCAG(16/14) BsiHKAI 112 GAGCTC G[AT]GC[AT]^C BsmAI 157 GAGAC GTCTC(1/5) BsmBI 157 GAGACG CGTCTC(1/5) BsoBI 5 CCCGGG C^[CT]CG[GA]G BspHI 65 TCATGA T^CATGA BstEII 384 GGTGACC G^GT[ACGT]ACC BstF5I 351 CATCC GGATG(2/0) BstPI 384 GGTGACC G^GT[ACGT]ACC DsaI 25 CCGCGG C^C[GA][CT]GG EarI 61 CTCTTC CTCTTC(1/4) EcoO65I 384 GGTGACC G^GT[ACGT]ACC FokI 351 CATCC GGATG(9/13) HgaI 159 GACGC GACGC(5/10) Ksp632I 61 CTCTTC CTCTTC(1/4) KspI 25 CCGCGG CCGC^GG MslI 378 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 25 CCGCGG C[AC]G^C[GT]G PpuMI 335 GGGACCC [GA]G^G[AT]CC[CT] RcaI 65 TCATGA T^CATGA SacI 112 GAGCTC GAGCT^C SacII 25 CCGCGG CCGC^GG SfaNI 350 GCATC GCATC(5/9) SfcI 195 CTACAG C^T[GA][CT]AG SmaI 5 CCCGGG CCC^GGG XcmI 254 CCAATGCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 5 CCCGGG C^CCGGG XmaI 5 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccII 26 CGCG CG^CG 31 CGCG BanII 8 GGGCCC G[GA]GC[CT]^C 112 GAGCTC BsaJI 5 CCCGGG C^C[ACGT][ACGT]GG 25 CCGCGG BsmFI 335 GGGAC GGGAC(10/14) 373 GTCCC BstUI 26 CGCG CG^CG 31 CGCG CfoI 30 GCGC GCG^C 426 GCGC DdeI 318 CTCAG C^T[ACGT]AG 431 CTGAG DraII 8 GGGCCCT [GA]G^G[ACGT]CC[CT] 335 GGGACCC EaeI 206 TGGCCG [CT]^GGCC[GA] 291 TGGCCG EcoO109I 8 GGGCCCT [GA]G^G[ACGT]CC[CT] 335 GGGACCC HhaI 30 GCGC GCG^C 426 GCGC HinP1I 30 GCGC G^CGC 426 GCGC MboII 62 TCTTC GAAGA(8/7) 275 TCTTC MvnI 26 CGCG CG^CG 31 CGCG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI AvaII BbvI BcnI BmyI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I EcoRII FauI Fnu4HI HaeIII HapII HpaII HphI ItaI MaeIII MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI Tsp45I TspRI