Restriction map for
pFN21AE4389
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AgeI 398 ACCGGT A^CCGGT
AocI 498 CCTGAGG CC^T[ACGT]AGG
AspHI 25 GAGCAC G[AT]GC[AT]^C
AvaI 16 CCCGAG C^[CT]CG[GA]G
BalI 193 TGGCCA TGG^CCA
BbsI 256 GTCTTC GAAGAC(2/6)
BciVI 62 GTATCC GTATCC(6/5)
BpmI 1012 CTGGAG CTGGAG(16/14)
BpuAI 256 GTCTTC GAAGAC(2/6)
BsiHKAI 25 GAGCAC G[AT]GC[AT]^C
BsmAI 118 GAGAC GTCTC(1/5)
BsmI 682 GCATTC GAATGC(1/-1)
BsoBI 16 CCCGAG C^[CT]CG[GA]G
BsrBI 568 GAGCGG CCGCTC(-3/-3)
BsrFI 398 ACCGGT [GA]^CCGG[CT]
BstXI 882 CCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 498 CCTGAGG CC^T[ACGT]AGG
Cfr10I 398 ACCGGT [GA]^CCGG[CT]
DpnII 1023 GATC ^GATC
DsaI 1155 CCGTGG C^C[GA][CT]GG
EaeI 193 TGGCCA [CT]^GGCC[GA]
EarI 43 CTCTTC CTCTTC(1/4)
Eco81I 498 CCTGAGG CC^T[ACGT]AGG
HaeII 808 AGCGCC [GA]GCGC^[CT]
HincII 186 GTTGAC GT[CT]^[GA]AC
HindII 186 GTTGAC GT[CT]^[GA]AC
Ksp632I 43 CTCTTC CTCTTC(1/4)
MaeII 788 ACGT A^CGT
MboI 1023 GATC ^GATC
MluNI 193 TGGCCA TGG^CCA
MscI 193 TGGCCA TGG^CCA
NdeII 1023 GATC ^GATC
PinAI 398 ACCGGT A^CCGGT
SapI 42 GCTCTTC GCTCTTC(1/4)
Sau3AI 1023 GATC ^GATC
StuI 290 AGGCCT AGG^CCT
TfiI 301 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 1057 CGCG CG^CG
1059 CGCG
AfaI 54 GTAC GT^AC
360 GTAC
BcnI 687 CCGGG CC^[GC]GG
984 CCGGG
BglI 128 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
496 GCCCTGAGGGC
BmyI 25 GAGCAC G[AGT]GC[ACT]^C
329 GTGCCC
BsaWI 398 ACCGGT [AT]^CCGG[AT]
834 ACCGGA
Bsp1286I 25 GAGCAC G[AGT]GC[ACT]^C
329 GTGCCC
BspMI 56 ACCTGC ACCTGC(4/8)
795 ACCTGC
BstAPI 505 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
702 GCAGAGGCTGC
BstUI 1057 CGCG CG^CG
1059 CGCG
CfoI 809 GCGC GCG^C
1058 GCGC
DraII 12 GGGTCCC [GA]G^G[ACGT]CC[CT]
1067 GGGTCCC
EcoNI 516 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
726 CCTGAAAGAGG
EcoO109I 12 GGGTCCC [GA]G^G[ACGT]CC[CT]
1067 GGGTCCC
EcoT14I 456 CCAAGG C^C[AT][AT]GG
964 CCTTGG
FauI 125 CCCGC CCCGC(4/6)
588 GCGGG
HgaI 39 GACGC GACGC(5/10)
347 GCGTC
HhaI 809 GCGC GCG^C
1058 GCGC
HinP1I 809 GCGC G^CGC
1058 GCGC
MboII 44 TCTTC GAAGA(8/7)
257 TCTTC
MvnI 1057 CGCG CG^CG
1059 CGCG
NciI 687 CCGGG CC^[GC]GG
984 CCGGG
PflMI 357 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
456 CCAAGGCATGG
PpuMI 12 GGGTCCC [GA]G^G[AT]CC[CT]
1067 GGGTCCC
PstI 709 CTGCAG CTGCA^G
868 CTGCAG
RsaI 54 GTAC GT^AC
360 GTAC
SfcI 709 CTGCAG C^T[GA][CT]AG
868 CTGCAG
StyI 456 CCAAGG C^C[AT][AT]GG
964 CCTTGG
Van91I 357 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
456 CCAAGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BbvI BfaI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsrI BssKI
BstEII BstF5I BstNI BstPI Cac8I
Cfr13I DdeI EcoO65I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MnlI
MslI MspA1I MspI MvaI MwoI
NlaIII NlaIV PleI PvuII Sau96I
ScrFI SfaNI TseI Tsp45I TspRI