Restriction map for
pFN21AE3924
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI Ksp632I MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Alw44I 876 GTGCAC G^TGCAC
ApaI 695 GGGCCC GGGCC^C
ApaLI 876 GTGCAC G^TGCAC
AspHI 876 GTGCAC G[AT]GC[AT]^C
BalI 572 TGGCCA TGG^CCA
BanII 695 GGGCCC G[GA]GC[CT]^C
BbvCI 606 GCTGAGG CCTCAGC(-5/-2)
BciVI 248 GGATAC GTATCC(6/5)
BglI 396 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 594 CACGTA [CT]AC^GT[GA]
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiHKAI 876 GTGCAC G[AT]GC[AT]^C
BsmAI 825 GTCTC GTCTC(1/5)
BsmFI 980 GTCCC GGGAC(10/14)
BspMI 311 ACCTGC ACCTGC(4/8)
BstYI 615 AGATCC [GA]^GATC[CT]
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
MflI 615 AGATCC [GA]^GATC[CT]
MluNI 572 TGGCCA TGG^CCA
MscI 572 TGGCCA TGG^CCA
MslI 228 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 752 CCATGG C^CATGG
PflMI 198 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 763 CAGCTG CAG^CTG
ScaI 56 AGTACT AGT^ACT
SexAI 740 ACCTGGT A^CC[AT]GGT
SphI 294 GCATGC GCATG^C
Van91I 198 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 615 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 91 GGCGCC G[GA]^CG[CT]C
642 GGCGCC
AflIII 252 ACATGT A^C[GA][CT]GT
531 ACATGT
AocI 621 CCTCAGG CC^T[ACGT]AGG
690 CCTGAGG
BbeI 91 GGCGCC GGCGC^C
642 GGCGCC
BbsI 490 GTCTTC GAAGAC(2/6)
559 GTCTTC
BpuAI 490 GTCTTC GAAGAC(2/6)
559 GTCTTC
BsaHI 91 GGCGCC G[GA]^CG[CT]C
642 GGCGCC
BspLU11I 252 ACATGT A^CATGT
531 ACATGT
Bsu36I 621 CCTCAGG CC^T[ACGT]AGG
690 CCTGAGG
Eco81I 621 CCTCAGG CC^T[ACGT]AGG
690 CCTGAGG
EcoT14I 752 CCATGG C^C[AT][AT]GG
842 CCTTGG
HgaI 288 GACGC GACGC(5/10)
435 GCGTC
Hin1I 91 GGCGCC G[GA]^CG[CT]C
642 GGCGCC
KasI 91 GGCGCC G^GCGCC
642 GGCGCC
KspI 63 CCGCGG CCGC^GG
816 CCGCGG
MaeII 218 ACGT A^CGT
595 ACGT
MaeIII 286 GTGAC ^GT[ACGT]AC
437 GTCAC
MboII 491 TCTTC GAAGA(8/7)
560 TCTTC
NarI 91 GGCGCC GG^CGCC
642 GGCGCC
PpuMI 626 GGGTCCT [GA]G^G[AT]CC[CT]
686 AGGACCT
PstI 349 CTGCAG CTGCA^G
760 CTGCAG
SacII 63 CCGCGG CCGC^GG
816 CCGCGG
SfoI 91 GGCGCC GGC^GCC
642 GGCGCC
StyI 752 CCATGG C^C[AT][AT]GG
842 CCTTGG
Tsp45I 286 GTGAC ^GT[GC]AC
437 GTCAC
TspRI 366 CAGTG CA[GC]TG[ACGT][ACGT]^
731 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanI BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI Bsp1286I BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI