Restriction map for
pFN21AB0404
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I CelII CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MslI
MunI NarI NheI NotI NruI
NsiI NspI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 625 CGCG CG^CG
AcyI 374 GACGCC G[GA]^CG[CT]C
Alw44I 1652 GTGCAC G^TGCAC
Aor51HI 206 AGCGCT AGC^GCT
ApaLI 1652 GTGCAC G^TGCAC
AspI 858 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 560 CCCGGG C^[CT]CG[GA]G
BbsI 336 GAAGAC GAAGAC(2/6)
BbvCI 988 GCTGAGG CCTCAGC(-5/-2)
BfaI 835 CTAG C^TAG
BpuAI 336 GAAGAC GAAGAC(2/6)
BsaBI 126 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 374 GACGCC G[GA]^CG[CT]C
BsiEI 621 CGATCG CG[GA][CT]^CG
BsoBI 560 CCCGGG C^[CT]CG[GA]G
BspDI 619 ATCGAT AT^CGAT
BssSI 1677 CACGAG CACGAG(-5/-1)
BstBI 1076 TTCGAA TT^CGAA
BstUI 625 CGCG CG^CG
CfoI 207 GCGC GCG^C
ClaI 619 ATCGAT AT^CGAT
DraIII 707 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1627 CCATGG C^C[GA][CT]GG
Eco47III 206 AGCGCT AGC^GCT
EcoRI 361 GAATTC G^AATTC
HaeII 206 AGCGCT [GA]GCGC^[CT]
HgaI 374 GACGC GACGC(5/10)
HhaI 207 GCGC GCG^C
Hin1I 374 GACGCC G[GA]^CG[CT]C
HinP1I 207 GCGC G^CGC
MaeI 835 CTAG C^TAG
MamI 126 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 625 CGCG CG^CG
NaeI 984 GCCGGC GCC^GGC
NcoI 1627 CCATGG C^CATGG
NdeI 356 CATATG CA^TATG
NgoMIV 984 GCCGGC G^CCGGC
NspV 1076 TTCGAA TT^CGAA
PflFI 858 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 879 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1175 CTGCAG CTGCA^G
PvuI 621 CGATCG CGAT^CG
PvuII 227 CAGCTG CAG^CTG
SfuI 1076 TTCGAA TT^CGAA
SmaI 560 CCCGGG CCC^GGG
SmlI 1687 CTCAAG C^T[CT][GA]AG
StuI 479 AGGCCT AGG^CCT
TspRI 711 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 858 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 879 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 860 CCAAGTCTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 560 CCCGGG C^CCGGG
XmaI 560 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 198 GTAC GT^AC
762 GTAC
AlwNI 1025 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1291 CAGTTACTG
AocI 754 CCTGAGG CC^T[ACGT]AGG
1009 CCTGAGG
AspHI 1034 GTGCTC G[AT]GC[AT]^C
1652 GTGCAC
BamHI 290 GGATCC G^GATCC
1325 GGATCC
BglII 831 AGATCT A^GATCT
1694 AGATCT
BsaI 856 GAGACC GGTCTC(1/5)
1346 GAGACC
BsiHKAI 1034 GTGCTC G[AT]GC[AT]^C
1652 GTGCAC
BsrBI 1381 GAGCGG CCGCTC(-3/-3)
1453 GAGCGG
BsrDI 527 GCAATG GCAATG(2/0)
1465 GCAATG
BstXI 279 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
516 CCAGACCTATGG
Bsu36I 754 CCTGAGG CC^T[ACGT]AGG
1009 CCTGAGG
Eco81I 754 CCTGAGG CC^T[ACGT]AGG
1009 CCTGAGG
MspA1I 227 CAGCTG C[AC]G^C[GT]G
1747 CAGCGG
PpuMI 998 AGGACCC [GA]G^G[AT]CC[CT]
1814 GGGACCT
RsaI 198 GTAC GT^AC
762 GTAC
Tsp45I 587 GTGAC ^GT[GC]AC
737 GTCAC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TthHB8I XhoII