Restriction map for
pFN21AE5235
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsgI BsiHKAI
BsiWI BsmBI BsmFI Bsp1407I BspHI
BsrBI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MscI MseI MunI MvnI
NarI NcoI NdeI NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 122 TCCGGA T^CCGGA
AflIII 353 ACATGT A^C[GA][CT]GT
AlwI 120 GATCC GGATC(4/5)
Asp700I 387 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 176 CCCGGG C^[CT]CG[GA]G
AvaII 168 GGTCC G^G[AT]CC
BanII 179 GGGCTC G[GA]GC[CT]^C
BbvCI 475 GCTGAGG CCTCAGC(-5/-2)
BbvI 312 GCTGC GCAGC(8/12)
BclI 146 TGATCA T^GATCA
BfaI 35 CTAG C^TAG
BglI 209 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 179 GGGCTC G[AGT]GC[ACT]^C
BpmI 529 CTCCAG CTGGAG(16/14)
BsaWI 122 TCCGGA [AT]^CCGG[AT]
BseAI 122 TCCGGA T^CCGGA
BsiEI 345 CGATCG CG[GA][CT]^CG
BsmI 263 GAATGC GAATGC(1/-1)
BsoBI 176 CCCGGG C^[CT]CG[GA]G
Bsp1286I 179 GGGCTC G[AGT]GC[ACT]^C
BspDI 343 ATCGAT AT^CGAT
BspEI 122 TCCGGA T^CCGGA
BspLU11I 353 ACATGT A^CATGT
BspMI 485 GCAGGT ACCTGC(4/8)
BsrFI 308 GCCGGC [GA]^CCGG[CT]
BstYI 119 AGATCC [GA]^GATC[CT]
Cfr10I 308 GCCGGC [GA]^CCGG[CT]
ClaI 343 ATCGAT AT^CGAT
FbaI 146 TGATCA T^GATCA
Fnu4HI 312 GCTGC GC^[ACGT]GC
HphI 378 GGTGA GGTGA(8/7)
ItaI 312 GCTGC GC^[ACGT]GC
MaeI 35 CTAG C^TAG
MflI 119 AGATCC [GA]^GATC[CT]
MroI 122 TCCGGA T^CCGGA
MslI 279 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 45 CCGCTG C[AC]G^C[GT]G
NaeI 308 GCCGGC GCC^GGC
NgoMIV 308 GCCGGC G^CCGGC
NspI 353 ACATGT [GA]CATG^[CT]
PvuI 345 CGATCG CGAT^CG
SapI 520 GAAGAGC GCTCTTC(1/4)
SfaNI 185 GATGC GCATC(5/9)
SmaI 176 CCCGGG CCC^GGG
TseI 312 GCTGC G^C[AT]GC
XhoII 119 AGATCC [GA]^GATC[CT]
XmaCI 176 CCCGGG C^CCGGG
XmaI 176 CCCGGG C^CCGGG
XmnI 387 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AfaI 248 GTAC GT^AC
369 GTAC
BcnI 176 CCCGG CC^[GC]GG
177 CCGGG
BsaI 82 GAGACC GGTCTC(1/5)
577 GAGACC
BseRI 288 GAGGAG GAGGAG(10/8)
544 GAGGAG
BsmAI 82 GAGAC GTCTC(1/5)
577 GAGAC
HaeII 512 AGCGCC [GA]GCGC^[CT]
524 AGCGCC
HinfI 331 GAATC G^A[ACGT]TC
408 GATTC
MaeIII 163 GTGAC ^GT[ACGT]AC
277 GTCAC
NciI 176 CCCGG CC^[GC]GG
177 CCGGG
RsaI 248 GTAC GT^AC
369 GTAC
TaqI 183 TCGA T^CGA
344 TCGA
TfiI 331 GAATC G^A[AT]TC
408 GATTC
Tsp45I 163 GTGAC ^GT[GC]AC
277 GTCAC
TthHB8I 183 TCGA T^CGA
344 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I FauI FokI HaeIII HapII
HhaI HinP1I HpaII Ksp632I MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII Sau3AI Sau96I ScrFI
StyI