Restriction map for
pFN21AE4474
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AlwNI ApoI AseI AsnI
Asp700I Asp718I AvrII BamHI BbrPI
BbvCI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstF5I
BstZ17I CelII ClaI CpoI DraI
DraIII EcoNI EcoRI EcoRV EcoT22I
FbaI FokI HindIII HpaI KpnI
MaeI MamI MfeI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SbfI SexAI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1234 GACGTC GACGT^C
AccI 517 GTCGAC GT^[AC][GT]AC
AhdI 349 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 400 GTGCAC G^TGCAC
AlwI 33 GATCC GGATC(4/5)
AocI 418 CCTCAGG CC^T[ACGT]AGG
ApaI 703 GGGCCC GGGCC^C
ApaLI 400 GTGCAC G^TGCAC
AscI 609 GGCGCGCC GG^CGCGCC
AspEI 349 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 372 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 616 CCCGAG C^[CT]CG[GA]G
AviII 185 TGCGCA TGC^GCA
BalI 1382 TGGCCA TGG^CCA
BbsI 1237 GTCTTC GAAGAC(2/6)
BcgI 753 CGAGCTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 131 AGATCT A^GATCT
BpuAI 1237 GTCTTC GAAGAC(2/6)
BseRI 859 GAGGAG GAGGAG(10/8)
BsmI 990 GAATGC GAATGC(1/-1)
BsoBI 616 CCCGAG C^[CT]CG[GA]G
BspHI 1130 TCATGA T^CATGA
BspMI 235 ACCTGC ACCTGC(4/8)
BstEII 1320 GGTCACC G^GT[ACGT]ACC
BstPI 1320 GGTCACC G^GT[ACGT]ACC
Bsu36I 418 CCTCAGG CC^T[ACGT]AGG
DraII 702 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 1312 GACTCCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 349 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1123 CTCTTC CTCTTC(1/4)
Eco81I 418 CCTCAGG CC^T[ACGT]AGG
EcoO109I 702 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1320 GGTCACC G^GT[ACGT]ACC
EcoT14I 737 CCAAGG C^C[AT][AT]GG
FseI 473 GGCCGGCC GGCCGG^CC
FspI 185 TGCGCA TGC^GCA
Ksp632I 1123 CTCTTC CTCTTC(1/4)
MluI 101 ACGCGT A^CGCGT
MluNI 1382 TGGCCA TGG^CCA
MscI 1382 TGGCCA TGG^CCA
PflFI 372 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 828 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 289 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1130 TCATGA T^CATGA
SalI 517 GTCGAC G^TCGAC
SapI 1122 GCTCTTC GCTCTTC(1/4)
ScaI 938 AGTACT AGT^ACT
SfaNI 1031 GCATC GCATC(5/9)
SgrAI 322 CACCGGCG C[GA]^CCGG[CT]G
SmlI 718 CTCAAG C^T[CT][GA]AG
StuI 1436 AGGCCT AGG^CCT
StyI 737 CCAAGG C^C[AT][AT]GG
Tth111I 372 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 828 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 101 ACGCGT A^C[GA][CT]GT
815 ACGTGT
Aor51HI 449 AGCGCT AGC^GCT
551 AGCGCT
BcnI 253 CCCGG CC^[GC]GG
959 CCGGG
BglI 191 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
696 GCCGTCGGGGC
BsaI 862 GAGACC GGTCTC(1/5)
874 GAGACC
BsrBI 648 CCGCTC CCGCTC(-3/-3)
1221 CCGCTC
BssHII 467 GCGCGC G^CGCGC
610 GCGCGC
BstXI 734 CCACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1385 CCATCAAGGTGG
BstYI 32 AGATCC [GA]^GATC[CT]
131 AGATCT
Eco47III 449 AGCGCT AGC^GCT
551 AGCGCT
HincII 424 GTCAAC GT[CT]^[GA]AC
517 GTCGAC
HindII 424 GTCAAC GT[CT]^[GA]AC
517 GTCGAC
KspI 1164 CCGCGG CCGC^GG
1316 CCGCGG
MflI 32 AGATCC [GA]^GATC[CT]
131 AGATCT
NaeI 474 GCCGGC GCC^GGC
621 GCCGGC
NciI 253 CCCGG CC^[GC]GG
959 CCGGG
NgoMIV 474 GCCGGC G^CCGGC
621 GCCGGC
PstI 307 CTGCAG CTGCA^G
793 CTGCAG
SacI 754 GAGCTC GAGCT^C
1120 GAGCTC
SacII 1164 CCGCGG CCGC^GG
1316 CCGCGG
XhoII 32 AGATCC [GA]^GATC[CT]
131 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BanII BbeI
BbvI BmyI BpmI BsaHI BsaJI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EagI EclXI Eco52I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SfoI TaqI TseI Tsp45I
TspRI TthHB8I